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K0EWT7 (K0EWT7_9NOCA) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 10. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
ORF Names:O3I_031645 EMBL AFU04278.1
OrganismNocardia brasiliensis ATCC 700358 [Complete proteome] EMBL AFU04278.1
Taxonomic identifier1133849 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain132 – 323192ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding158 – 20851ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2771Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2901Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2901Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2921Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
K0EWT7 [UniParc].

Last modified November 28, 2012. Version 1.
Checksum: CF427CD3CB3ABC20

FASTA33035,179
        10         20         30         40         50         60 
MQVAVLFGGT NAERDVSIAT AGQVFRALRT RGHEVSAYDT TAGRLDADGE DRLMAATVPA 

        70         80         90        100        110        120 
EPPQVSGAVP DVTMRLLCEQ GGLRDVDVVF LALHGGTGEN GTMQGLLDLA GVPYTGSGVL 

       130        140        150        160        170        180 
ASAMAMDKDV SKRLFRLAGV PTPDWEMAAD EFDDRLGWPV IVKPNKQGST VGLTVVKDPA 

       190        200        210        220        230        240 
QYAEAVRVAR AHDDEVMVER FVPGREFVVG VLGDDPLAVG EIIPQGSEIF DYRSKYQVGG 

       250        260        270        280        290        300 
AVEIFPAEIP AELADEMRAQ AVRAHRALKL GAYSRVDFRV DAAGDVWCLE ANTLPGMTAT 

       310        320        330 
SLLPQSAAAA GIDFAELCEQ ICLHALRKLD 

« Hide

References

[1]"Complete genome sequence of Nocardia brasiliensis HUJEG-1."
Vera-Cabrera L., Ortiz-Lopez R., Elizondo-Gonzalez R., Perez-Maya A.A., Ocampo-Candiani J.
J. Bacteriol. 194:2761-2762(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HUJEG-1 EMBL AFU04278.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003876 Genomic DNA. Translation: AFU04278.1.
RefSeqYP_006811252.1. NC_018681.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFU04278; AFU04278; O3I_031645.
GeneID13734832.
KEGGnbr:O3I_031645.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK0EWT7_9NOCA
AccessionPrimary (citable) accession number: K0EWT7
Entry history
Integrated into UniProtKB/TrEMBL: November 28, 2012
Last sequence update: November 28, 2012
Last modified: June 11, 2014
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)