ID   K0DJM2_9BURK            Unreviewed;      1055 AA.
AC   K0DJM2;
DT   28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT   28-NOV-2012, sequence version 1.
DT   24-JAN-2024, entry version 54.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN   ORFNames=BUPH_05369 {ECO:0000313|EMBL:AFT84977.1};
OS   Paraburkholderia phenoliruptrix BR3459a.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=1229205 {ECO:0000313|EMBL:AFT84977.1, ECO:0000313|Proteomes:UP000010105};
RN   [1] {ECO:0000313|EMBL:AFT84977.1, ECO:0000313|Proteomes:UP000010105}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BR3459a {ECO:0000313|EMBL:AFT84977.1,
RC   ECO:0000313|Proteomes:UP000010105};
RX   PubMed=23144415; DOI=10.1128/JB.01821-12;
RA   de Oliveira Cunha C., Goda Zuleta L.F., Paula de Almeida L.G.,
RA   Prioli Ciapina L., Lustrino Borges W., Pitard R.M., Baldani J.I.,
RA   Straliotto R., de Faria S.M., Hungria M., Sousa Cavada B., Mercante F.M.,
RA   Ribeiro de Vasconcelos A.T.;
RT   "Complete Genome Sequence of Burkholderia phenoliruptrix BR3459a (CLA1), a
RT   Heat-Tolerant, Nitrogen-Fixing Symbiont of Mimosa flocculosa.";
RL   J. Bacteriol. 194:6675-6676(2012).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC       ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR   EMBL; CP003863; AFT84977.1; -; Genomic_DNA.
DR   AlphaFoldDB; K0DJM2; -.
DR   STRING; 1229205.BUPH_05369; -.
DR   KEGG; bpx:BUPH_05369; -.
DR   PATRIC; fig|1229205.11.peg.960; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_4; -.
DR   Proteomes; UP000010105; Chromosome 1.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW   Rule:MF_00595};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Pyruvate {ECO:0000313|EMBL:AFT84977.1}.
FT   REGION          1..101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..21
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        64..88
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        265
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        707
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   1055 AA;  115696 MW;  C9CBC3EF429A5F87 CRC64;
     MTSSGSARSA RRNTASPNAS LDDAAASPAA ASSTVETAQP PKAKRATSGA KAANAAKAVK
     AEKTSAPVKA DKTVKTSKAD KSGEAAPSAK KGKAKAAGKA PVLQAVSEDT VMAAPKTNGR
     TRDDKDHPLF QDIRYLGRLL GDVLREQEGD AVFDVVETIR QTAVRFRRED DNAAAQTLDK
     KLRSLSPEQT VSVVRAFSYF SHLANIAEDR HRNRRHRIHA LAGSAAQPGS IAYALERLVE
     AGAAATPVLQ QFFNDALIVP VLTAHPTEVQ RKSILDAEHD VARLLAERDQ QLTDRERAQN
     ERMLRARVTS LWQTRMLRDS RLTVADEIEN ALSYYRATFL EEIPALYADI EEALKEHGLE
     ARLPPFFQMG SWIGGDRDGN PNVTAETLEH AIARQAEVIF EHYLEQVHKL GAELSVSNLL
     AGASDELKAL AEISPDRSPH RTDEPYRRAL IGMYTRLAAS ARVRLGEGAV PLRSAGRGAA
     PIRATPYDDA SEFVRDLHVL MDSLAAHHGA PLAAPRLAPL ARAAEVFGFH LASIDLRQSS
     DIHEAVIAEL LKRAGVHDDY AALDESAKLE VLLAELAQPR PLRLPYAEYS DLVKSELSVL
     EQARVTREKF GARAVRNYII SHTETVSDLV EVMLLQKETG LLQGQLGNAD NPAKAALMVI
     PLFETIPDLR NAPHIMRDLL ALPGADSIIE HQGNEQEVML GYSDSNKDGG FLTSNWELYR
     AELALVSLFN ERGITLRLFH GRGGTVGRGG GPTYQAILSQ PPGTVDGQIR LTEQGEVIAS
     KFGNPEIGRR NLETVVAATL EASLLPHGNA PADLPAFEET MQQLSDAAMA SYRALVYETP
     GFKEYFFEST PISEIAELNI GSRPASRKLQ DPKHRKIEDL RAIPWGFSWG QCRLLLTGWY
     GFGSAVAAYL DSAPSDAERG RRLSLLKKMH KSWPFFSTLL SNMDMVLAKT DLAVASRYAA
     LVSDKKLRKH VFERIVAEWE RTSKVLSEIT GKRERLAENP LLARSIKNRF PYLDPLNHLQ
     VELLKRHRAG DTNARVRRGI HLTINGIAAG LRNTG
//