ID   J9Y1K3_CUCSA            Unreviewed;       965 AA.
AC   J9Y1K3;
DT   28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT   28-NOV-2012, sequence version 1.
DT   24-JAN-2024, entry version 40.
DE   RecName: Full=phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00012305};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305};
GN   Name=ppc1 {ECO:0000313|EMBL:AFS33790.1};
GN   ORFNames=Csa_4G627210 {ECO:0000313|EMBL:KGN55071.1};
OS   Cucumis sativus (Cucumber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX   NCBI_TaxID=3659 {ECO:0000313|EMBL:AFS33790.1};
RN   [1] {ECO:0000313|EMBL:KGN55071.1, ECO:0000313|Proteomes:UP000029981}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 9930 {ECO:0000313|Proteomes:UP000029981};
RX   PubMed=19881527; DOI=10.1038/ng.475;
RA   Huang S., Li R., Zhang Z., Li L., Gu X., Fan W., Lucas W.J., Wang X.,
RA   Xie B., Ni P., Ren Y., Zhu H., Li J., Lin K., Jin W., Fei Z., Li G.,
RA   Staub J., Kilian A., van der Vossen E.A., Wu Y., Guo J., He J., Jia Z.,
RA   Ren Y., Tian G., Lu Y., Ruan J., Qian W., Wang M., Huang Q., Li B.,
RA   Xuan Z., Cao J., Asan null, Wu Z., Zhang J., Cai Q., Bai Y., Zhao B.,
RA   Han Y., Li Y., Li X., Wang S., Shi Q., Liu S., Cho W.K., Kim J.Y., Xu Y.,
RA   Heller-Uszynska K., Miao H., Cheng Z., Zhang S., Wu J., Yang Y., Kang H.,
RA   Li M., Liang H., Ren X., Shi Z., Wen M., Jian M., Yang H., Zhang G.,
RA   Yang Z., Chen R., Liu S., Li J., Ma L., Liu H., Zhou Y., Zhao J., Fang X.,
RA   Li G., Fang L., Li Y., Liu D., Zheng H., Zhang Y., Qin N., Li Z., Yang G.,
RA   Yang S., Bolund L., Kristiansen K., Zheng H., Li S., Zhang X., Yang H.,
RA   Wang J., Sun R., Zhang B., Jiang S., Wang J., Du Y., Li S.;
RT   "The genome of the cucumber, Cucumis sativus L.";
RL   Nat. Genet. 41:1275-1281(2009).
RN   [2] {ECO:0000313|EMBL:KGN55071.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=19495411; DOI=10.1371/journal.pone.0005795;
RA   Ren Y., Zhang Z., Liu J., Staub J.E., Han Y., Cheng Z., Li X., Lu J.,
RA   Miao H., Kang H., Xie B., Gu X., Wang X., Du Y., Jin W., Huang S.;
RT   "An integrated genetic and cytogenetic map of the cucumber genome.";
RL   PLoS ONE 4:E5795-E5795(2009).
RN   [3] {ECO:0000313|EMBL:KGN55071.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=20565788; DOI=10.1186/1471-2164-11-384;
RA   Guo S., Zheng Y., Joung J.G., Liu S., Zhang Z., Crasta O.R., Sobral B.W.,
RA   Xu Y., Huang S., Fei Z.;
RT   "Transcriptome sequencing and comparative analysis of cucumber flowers with
RT   different sex types.";
RL   BMC Genomics 11:384-384(2010).
RN   [4] {ECO:0000313|EMBL:KGN55071.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=22047402; DOI=10.1186/1471-2164-12-540;
RA   Li Z., Zhang Z., Yan P., Huang S., Fei Z., Lin K.;
RT   "RNA-Seq improves annotation of protein-coding genes in the cucumber
RT   genome.";
RL   BMC Genomics 12:540-540(2011).
RN   [5] {ECO:0000313|EMBL:AFS33790.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Guo F., Tan Z., Liu X., Liu L., Ren H.;
RT   "Transcriptional Analyzing of Cs-ppc Genes and Their Response to Light
RT   Intensity in Cucumber Leaves.";
RL   Hua Bei Nong Xue Bao 26:21-24(2011).
RN   [6] {ECO:0000313|EMBL:AFS33790.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Guo F., Tan Z., Liu X., Liu L., Ren H.;
RL   Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases.
RN   [7] {ECO:0000313|EMBL:KGN55071.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Huang S., Zhang Z., Lin K., Zhou Q.;
RL   Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Through the carboxylation of phosphoenolpyruvate (PEP) it
CC       forms oxaloacetate, a four-carbon dicarboxylic acid source for the
CC       tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003774}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346}.
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DR   EMBL; JX317639; AFS33790.1; -; mRNA.
DR   EMBL; CM002925; KGN55071.1; -; Genomic_DNA.
DR   RefSeq; NP_001267517.1; NM_001280588.1.
DR   RefSeq; XP_011652896.1; XM_011654594.1.
DR   AlphaFoldDB; J9Y1K3; -.
DR   STRING; 3659.J9Y1K3; -.
DR   EnsemblPlants; KGN55071; KGN55071; Csa_4G627210.
DR   GeneID; 101208329; -.
DR   Gramene; KGN55071; KGN55071; Csa_4G627210.
DR   KEGG; csv:101208329; -.
DR   eggNOG; ENOG502QPVS; Eukaryota.
DR   OMA; WAFGEQL; -.
DR   OrthoDB; 355614at2759; -.
DR   Proteomes; UP000029981; Chromosome 4.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IBA:GO_Central.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   GO; GO:0048366; P:leaf development; IBA:GO_Central.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF33; PHOSPHOENOLPYRUVATE CARBOXYLASE 3; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   2: Evidence at transcript level;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW   Pyruvate {ECO:0000313|EMBL:AFS33790.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000029981}.
FT   ACT_SITE        172
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        600
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   965 AA;  110195 MW;  A8A2EA9597804557 CRC64;
     MANRNLEKMA SIDAQLRLLV PARVSEDDKL VEYDALLLDR FLDILQDLHG EDLKETVQEC
     YELSAEYEGK FNPKKLEELG NVLTSLDPGD SIVIAKSFSH MLNLANLAEE VQIAYRRRIK
     LKKGDFADEN SATTESDIEE TLKKLVGELK KSPQEVFDAL KNQTVDLVLT AHPTQSVRRS
     LLQKHARIRD CLVQLYAKDI TPDDKQELDE ALQREIQAAF RTDEIRRTPP TPQDEMRAGM
     SYFHETIWKG VPKFLRRVDT ALKNIGIDER VPYNAPLIQF SSWMGGDRDG NPRVTPEVTR
     DVCLLARMMA ANLYYSQIED LMFELSMWRC SNELRERADV LHNSSRRDAK HYIEFWKQVP
     ASEPYRVILG DVRDKLYQTR ERSRHLLANG YSDIPEDATF TNVEQFLEPL ELCYRSLCAC
     GDRAIADGTL LDFLRQVSTF GLSLVRLDIR QESDRHTDVL DAITQHLDIG SYKEWSEEQR
     QEWLLSELSG KRPLFGPDLP TTEEISDVLN TFHVIAELPS DNFGAYIISM ATAPSDVLAV
     ELLQRECHVS QPLRVVPLFE KLADLEAAPA ALARLFSIDW YRNRINGKQE VMIGYSDSGK
     DAGRFSAAWQ LYKAQEELIK VAKQYGVKLT MFHGRGGTVG RGGGPTHLAI LSQPPETVHG
     SLRVTVQGEV IEQSFGEEHL CFRTLQRFTA ATLEHGMHPP VSPKPEWRAL MDEMAVVATE
     EYRSIVFKEP RFVEYFRLAT PELEYGRMNI GSRPSKRKPS GGIESLRAIP WIFAWTQTRF
     HLPVWLGFGA AFKHIIQKNV KNLQMLQEMY NEWPFFRVTI DLVEMVFAKG DPGIAALYDK
     LLVSEDLWSF GERLRANYEE TKSLLLKIAG HSDLLEGDPY LKQRLRLRDS YITTLNVCQA
     YTLKRIRDPN YHVKVRPHIS KEIMEASKPA DELIHLNPQS EYAPGLEDTL ILTMKGIAAG
     MQNTG
//