ID   J9XXT8_CUCSA            Unreviewed;       965 AA.
AC   J9XXT8;
DT   28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT   28-NOV-2012, sequence version 1.
DT   24-JAN-2024, entry version 42.
DE   RecName: Full=phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00012305};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305};
GN   Name=ppc2 {ECO:0000313|EMBL:AFS33791.1};
GN   ORFNames=Csa_7G048110 {ECO:0000313|EMBL:KGN43627.1};
OS   Cucumis sativus (Cucumber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX   NCBI_TaxID=3659 {ECO:0000313|EMBL:AFS33791.1};
RN   [1] {ECO:0000313|EMBL:KGN43627.1, ECO:0000313|Proteomes:UP000029981}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 9930 {ECO:0000313|Proteomes:UP000029981};
RX   PubMed=19881527; DOI=10.1038/ng.475;
RA   Huang S., Li R., Zhang Z., Li L., Gu X., Fan W., Lucas W.J., Wang X.,
RA   Xie B., Ni P., Ren Y., Zhu H., Li J., Lin K., Jin W., Fei Z., Li G.,
RA   Staub J., Kilian A., van der Vossen E.A., Wu Y., Guo J., He J., Jia Z.,
RA   Ren Y., Tian G., Lu Y., Ruan J., Qian W., Wang M., Huang Q., Li B.,
RA   Xuan Z., Cao J., Asan null, Wu Z., Zhang J., Cai Q., Bai Y., Zhao B.,
RA   Han Y., Li Y., Li X., Wang S., Shi Q., Liu S., Cho W.K., Kim J.Y., Xu Y.,
RA   Heller-Uszynska K., Miao H., Cheng Z., Zhang S., Wu J., Yang Y., Kang H.,
RA   Li M., Liang H., Ren X., Shi Z., Wen M., Jian M., Yang H., Zhang G.,
RA   Yang Z., Chen R., Liu S., Li J., Ma L., Liu H., Zhou Y., Zhao J., Fang X.,
RA   Li G., Fang L., Li Y., Liu D., Zheng H., Zhang Y., Qin N., Li Z., Yang G.,
RA   Yang S., Bolund L., Kristiansen K., Zheng H., Li S., Zhang X., Yang H.,
RA   Wang J., Sun R., Zhang B., Jiang S., Wang J., Du Y., Li S.;
RT   "The genome of the cucumber, Cucumis sativus L.";
RL   Nat. Genet. 41:1275-1281(2009).
RN   [2] {ECO:0000313|EMBL:KGN43627.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=19495411; DOI=10.1371/journal.pone.0005795;
RA   Ren Y., Zhang Z., Liu J., Staub J.E., Han Y., Cheng Z., Li X., Lu J.,
RA   Miao H., Kang H., Xie B., Gu X., Wang X., Du Y., Jin W., Huang S.;
RT   "An integrated genetic and cytogenetic map of the cucumber genome.";
RL   PLoS ONE 4:E5795-E5795(2009).
RN   [3] {ECO:0000313|EMBL:KGN43627.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=20565788; DOI=10.1186/1471-2164-11-384;
RA   Guo S., Zheng Y., Joung J.G., Liu S., Zhang Z., Crasta O.R., Sobral B.W.,
RA   Xu Y., Huang S., Fei Z.;
RT   "Transcriptome sequencing and comparative analysis of cucumber flowers with
RT   different sex types.";
RL   BMC Genomics 11:384-384(2010).
RN   [4] {ECO:0000313|EMBL:KGN43627.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=22047402; DOI=10.1186/1471-2164-12-540;
RA   Li Z., Zhang Z., Yan P., Huang S., Fei Z., Lin K.;
RT   "RNA-Seq improves annotation of protein-coding genes in the cucumber
RT   genome.";
RL   BMC Genomics 12:540-540(2011).
RN   [5] {ECO:0000313|EMBL:AFS33791.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Guo F., Tan Z., Liu X., Liu L., Ren H.;
RT   "Transcriptional Analyzing of Cs-ppc Genes and Their Response to Light
RT   Intensity in Cucumber Leaves.";
RL   Hua Bei Nong Xue Bao 26:21-24(2011).
RN   [6] {ECO:0000313|EMBL:AFS33791.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Guo F., Tan Z., Liu X., Liu L., Ren H.;
RL   Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases.
RN   [7] {ECO:0000313|EMBL:KGN43627.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Huang S., Zhang Z., Lin K., Zhou Q.;
RL   Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346}.
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DR   EMBL; JX317640; AFS33791.1; -; mRNA.
DR   EMBL; CM002928; KGN43627.1; -; Genomic_DNA.
DR   RefSeq; NP_001267665.1; NM_001280736.1.
DR   RefSeq; XP_011658533.1; XM_011660231.1.
DR   AlphaFoldDB; J9XXT8; -.
DR   STRING; 3659.J9XXT8; -.
DR   EnsemblPlants; KGN43627; KGN43627; Csa_7G048110.
DR   GeneID; 101208202; -.
DR   Gramene; KGN43627; KGN43627; Csa_7G048110.
DR   KEGG; csv:101208202; -.
DR   eggNOG; ENOG502QPVS; Eukaryota.
DR   OMA; WKSIPTT; -.
DR   OrthoDB; 355614at2759; -.
DR   Proteomes; UP000029981; Chromosome 7.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IBA:GO_Central.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   GO; GO:0048366; P:leaf development; IBA:GO_Central.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF47; PHOSPHOENOLPYRUVATE CARBOXYLASE 2; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   2: Evidence at transcript level;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW   Pyruvate {ECO:0000313|EMBL:AFS33791.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000029981}.
FT   ACT_SITE        172
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        600
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   965 AA;  110103 MW;  83CA551F79E2446B CRC64;
     MAAVKNLEKM ASIDAQLRLL APSKVSEDDK LVEYDALLLD RFLDILQDLH GEDLRETVQE
     CYEFAAEYER KRDPEKLEEL GNALTSLDPG DSIVVAKSFS HMLSLANLAE EVQIAYRRRI
     KLKKGGFADE ANATTESDIE ETFKRLLQLN KSPQEVFDAL KNQTVDLVFT AHPTQSVRRS
     LLQKHGRIRN CLTQLYAKDI TPDDKQELDE ALQREIQAAF RTDEIRRTPP TPQDEMRAGM
     SYFHETIWKG VPKFLRRVDT ALKNIGIDER VPYNAPLIQF SSWMGGDRDG NPRVTPEVTR
     DVCLLARMMA ANLYFSQIED LMFELSMWRC SSELQTRAEE LHRSSRKDAK HYIEFWKQIP
     PNEPYRVILG DVRDKLYNTR ERARHLLSSG MSEIPEDTTF TDVEQFLEPL ELCYRSLCAC
     GDRPIADGSL LDFLRQVSTF GLSLVRLDIR QESERHADVI DTITNYLGIG SYKEWSEEQK
     QEWLLSELSG KRPLFGPDLP KTEETSDVLD TFSVIAELPS DNFGAYIISM ATAPSDVLAV
     ELLQRECHVQ KPLRVVPLFE KLADLEAAPS AMARLFSIDW YRNRIDGKQE VMIGYSDSGK
     DAGRLSAAWQ LYKAQEELIQ VAKQYGVKLT MFHGRGGTVG RGGGPTHLAI LSQPPDTIHG
     SLRVTVQGEV IEQSFGEEHL CFRTLQRFTA ATLEHGMNPP VAPKPEWRAL LDAMAVVATE
     KYRSIVFQEP RFVEYFRLAT PELEYGRMNI GSRPSKRKPS GGIESLRAIP WIFAWTQTRF
     HLPVWLGFGA AFKYAIEKDV KNLHMLQEMY SQWPFFRVTI DLVEMVFAKG DPGIAALYDK
     LLVSEDLWSF GEQLRANYEE TKNLLLQVAK HKDLLEGDPY LRQRLRLRDS YITTLNVCQA
     YTLKRIRDPN YNVKVRPHLS KEYLESSKSA AELVKLNPQS EYAPGLEDTL ILTMKGIAAG
     MQNTG
//