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J9WK82 (J9WK82_9MYCO) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 9. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
ORF Names:MIP_05518 EMBL AFS15716.1
OrganismMycobacterium indicus pranii MTCC 9506 [Complete proteome] EMBL AFS15716.1
Taxonomic identifier1232724 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain164 – 371208ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding192 – 24756ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3261Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3381Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3381Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3401Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
J9WK82 [UniParc].

Last modified November 28, 2012. Version 1.
Checksum: 4580BF3A7D574228

FASTA38140,139
        10         20         30         40         50         60 
MNASDRSTPR PGSGGGERAR VAVVFGGRSN EHAISCVSAG SILRNLDPER FEVVAIGITP 

        70         80         90        100        110        120 
EGSWVLTDGD PAALAITDRR LPEVTAESGT ELALSADPRR GGQLVALPPG AGEVLASVDV 

       130        140        150        160        170        180 
VFPVLHGPYG EDGTIQGLLE LAGVPYVGAG VLASAAGMDK EFTKKLFAAE GLPVGDYAVL 

       190        200        210        220        230        240 
RPSRARLRPE ECQRLGLPVF VKPARGGSSI GVSRVASWDQ LDAAVAAARR HDPKVIVEAA 

       250        260        270        280        290        300 
IVGRELECGV LEMPDGTVEA SALGEIRVAG VRGREDSFYD FATKYLDDAA ELDVPAKVDD 

       310        320        330        340        350        360 
EIADTVRQLA VRAFKAIDCQ GLARVDFFLT EDGPVLNEIN TMPGFTTISM YPRMWAASGV 

       370        380 
DYPTLLATMV ETALARGVGL R 

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References

[1]"Molecular analysis of a leprosy immunotherapeutic bacillus provides insights into Mycobacterium evolution."
Ahmed N., Saini V., Raghuvanshi S., Khurana J.P., Tyagi A.K., Tyagi A.K., Hasnain S.E.
PLoS ONE 2:E968-E968(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Massive gene acquisitions in Mycobacterium indicus pranii provide a perspective on mycobacterial evolution."
Saini V., Raghuvanshi S., Khurana J.P., Ahmed N., Hasnain S.E., Tyagi A.K., Tyagi A.K.
Nucleic Acids Res. 40:10832-10850(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002275 Genomic DNA. Translation: AFS15716.1.
RefSeqYP_006730806.1. NC_018612.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFS15716; AFS15716; MIP_05518.
GeneID13661238.
KEGGmid:MIP_05518.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameJ9WK82_9MYCO
AccessionPrimary (citable) accession number: J9WK82
Entry history
Integrated into UniProtKB/TrEMBL: November 28, 2012
Last sequence update: November 28, 2012
Last modified: February 19, 2014
This is version 9 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)