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Protein

Probable DNA ligase

Gene

lig

Organism
Mycobacterium indicus pranii MTCC 9506
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei211 – 2111ATPUniRule annotation
Active sitei213 – 2131N6-AMP-lysine intermediateUniRule annotation
Binding sitei218 – 2181ATPUniRule annotation
Binding sitei233 – 2331ATPUniRule annotation
Binding sitei262 – 2621ATPUniRule annotation
Binding sitei302 – 3021ATPUniRule annotation
Binding sitei374 – 3741ATPUniRule annotation
Binding sitei380 – 3801ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
ORF Names:MIP_05705Imported
OrganismiMycobacterium indicus pranii MTCC 9506Imported
Taxonomic identifieri1232724 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
ProteomesiUP000007329: Chromosome

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

KOiK01971.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

J9WI19-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLLDVAKAS TDVGSTSSRL RKVAHIADLL ARAAPDPALV MIVVSWLSGE
60 70 80 90 100
LRQRQIGVGW AALRSRPPPA SHATLTVGAV DATFSEIGAV SGKGAQARRA
110 120 130 140 150
ALLTTLFAAA AEAEQTFLLR LLGGELRQGA LAGIMADAVA KAAGIPAAAV
160 170 180 190 200
QRAAMLGGDL PAVAAAALSG EPAALQAFTL RVGRPVGPML AQTAPSVADA
210 220 230 240 250
IERHGGRAIF EAKLDGARVQ IHRSGDQVTV YTRSLDDVTA RVPEVVEATL
260 270 280 290 300
ALPVNDLIAD GEAIALRPDN RPQRFQVTAS RFGRSVDVAA AVAAQPLSVF
310 320 330 340 350
FFDILHRDGV DLLDAPTTDR LAALDALVPP AQRVDRLLTS DPVEAGAFLD
360 370 380 390 400
ATLAVGHEGV MAKAPDAQYQ AGRRGAGWLK VKPVHTLDLV VLAVEWGSGR
410 420 430 440 450
RRGKLSNIHL GARDPDSGEF VMVGKTFKGM TDAMLDWQTA RFTDLAIGGT
460 470 480 490 500
DGYVVHVRPE QVVEVALDGV QKSSRYPGGL ALRFARVVRY RDDKGPAEAD

TIDAVRALY
Length:509
Mass (Da):53,533
Last modified:November 28, 2012 - v1
Checksum:iCCBD8ABDD5DF5F4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002275 Genomic DNA. Translation: AFS15833.1.
RefSeqiYP_006730923.1. NC_018612.1.

Genome annotation databases

EnsemblBacteriaiAFS15833; AFS15833; MIP_05705.
GeneIDi13661357.
KEGGimid:MIP_05705.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002275 Genomic DNA. Translation: AFS15833.1.
RefSeqiYP_006730923.1. NC_018612.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFS15833; AFS15833; MIP_05705.
GeneIDi13661357.
KEGGimid:MIP_05705.

Phylogenomic databases

KOiK01971.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular analysis of a leprosy immunotherapeutic bacillus provides insights into Mycobacterium evolution."
    Ahmed N., Saini V., Raghuvanshi S., Khurana J.P., Tyagi A.K., Tyagi A.K., Hasnain S.E.
    PLoS ONE 2:E968-E968(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Massive gene acquisitions in Mycobacterium indicus pranii provide a perspective on mycobacterial evolution."
    Saini V., Raghuvanshi S., Khurana J.P., Ahmed N., Hasnain S.E., Tyagi A.K., Tyagi A.K.
    Nucleic Acids Res. 40:10832-10850(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiJ9WI19_9MYCO
AccessioniPrimary (citable) accession number: J9WI19
Entry historyi
Integrated into UniProtKB/TrEMBL: November 28, 2012
Last sequence update: November 28, 2012
Last modified: February 4, 2015
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.