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J9W3A6

- J9W3A6_CRYNH

UniProt

J9W3A6 - J9W3A6_CRYNH

Protein

Probable acetate kinase

Gene

CNAG_06432

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 16 (01 Oct 2014)
      Sequence version 1 (28 Nov 2012)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + acetate = ADP + acetyl phosphate.UniRule annotation

    Cofactori

    Mg2+.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi12 – 121MagnesiumUniRule annotation
    Binding sitei19 – 191ATPUniRule annotation
    Binding sitei100 – 1001SubstrateUniRule annotation
    Active sitei159 – 1591Proton donor/acceptorUniRule annotation
    Sitei192 – 1921Transition state stabilizerUniRule annotation
    Sitei253 – 2531Transition state stabilizerUniRule annotation
    Metal bindingi416 – 4161MagnesiumUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi220 – 2245ATPUniRule annotation

    GO - Molecular functioni

    1. acetate kinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. acetyl-CoA biosynthetic process Source: UniProtKB-UniPathway
    2. organic acid metabolic process Source: InterPro

    Keywords - Molecular functioni

    KinaseUniRule annotationImported, Transferase

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00340; UER00458.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
    Alternative name(s):
    AcetokinaseUniRule annotation
    Gene namesi
    ORF Names:CNAG_06432Imported
    OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)Imported
    Taxonomic identifieri235443 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
    ProteomesiUP000010091: Chromosome 13

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4H0PX-ray1.89A/B1-430[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the acetokinase family.UniRule annotation

    Phylogenomic databases

    OrthoDBiEOG7X9GH2.

    Family and domain databases

    HAMAPiMF_00020. Acetate_kinase.
    InterProiIPR004372. Ac/Proprionate_kinase.
    IPR000890. Aliphatic_acid_kin_short-chain.
    IPR023865. Aliphatic_acid_kinase_CS.
    [Graphical view]
    PANTHERiPTHR21060. PTHR21060. 1 hit.
    PfamiPF00871. Acetate_kinase. 1 hit.
    [Graphical view]
    PRINTSiPR00471. ACETATEKNASE.
    TIGRFAMsiTIGR00016. ackA. 1 hit.
    PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    J9W3A6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPDKAEYLLA INCGSSSIKG KLFAIPSFEL LANLAVTNIS SSDERVKIKT    50
    TWEEGKGKDS EEEADYGDKI RYASLVPILL DHLTNSTHVK KEEIKYVCHR 100
    VVHGGMHDKG IRVVKGHEEG LMEMDKLSEF APLHNHRAVL AVKSCIDALP 150
    HHTSLLLFDT IFHRTIAPEV YTYALPPPDT ELTMPLRKYG FHGLSYASIV 200
    QSLAEHLKKP SDQINVVVAH LGSGSSSCCI KNGKSIDTSM GLTPLEGLLG 250
    GTRSGTIDPT AIFHHTEDAA SDANVGDFTV SKAEIILNKN SGFKALAGTT 300
    NFGHIIQNLD PSKCSEEDHE KAKLTYAVFL DRLLNFVAQY LFKLLSEVPI 350
    ESIDGLVFSG GIGEKGAELR RDVLKKLAWL GAEVDEEANN SNSGGAVKCI 400
    TKEGSKLKGW VVETDEEGWM ARMAKEEFGF 430
    Length:430
    Mass (Da):47,039
    Last modified:November 28, 2012 - v1
    Checksum:iF2835D3B6D7C8B05
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003832 Genomic DNA. Translation: AFR98670.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003832 Genomic DNA. Translation: AFR98670.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4H0P X-ray 1.89 A/B 1-430 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    OrthoDBi EOG7X9GH2.

    Enzyme and pathway databases

    UniPathwayi UPA00340 ; UER00458 .

    Family and domain databases

    HAMAPi MF_00020. Acetate_kinase.
    InterProi IPR004372. Ac/Proprionate_kinase.
    IPR000890. Aliphatic_acid_kin_short-chain.
    IPR023865. Aliphatic_acid_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR21060. PTHR21060. 1 hit.
    Pfami PF00871. Acetate_kinase. 1 hit.
    [Graphical view ]
    PRINTSi PR00471. ACETATEKNASE.
    TIGRFAMsi TIGR00016. ackA. 1 hit.
    PROSITEi PS01075. ACETATE_KINASE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans."
      Thaker T.M., Tanabe M., Fowler M.L., Preininger A.M., Ingram-Smith C., Smith K.S., Iverson T.M.
      J. Struct. Biol. 181:185-189(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS).
    2. "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation."
      Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V., Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F., Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A., Gaillardin C., Gerik K.J.
      , Goldberg J., Gonzalez-Hilarion S., Gujja S., Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L., Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K., Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A., Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A., Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A., Cuomo C.A., Dietrich F.S.
      PLoS Genet. 10:E1004261-E1004261(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487Imported.

    Entry informationi

    Entry nameiJ9W3A6_CRYNH
    AccessioniPrimary (citable) accession number: J9W3A6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 28, 2012
    Last sequence update: November 28, 2012
    Last modified: October 1, 2014
    This is version 16 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteomeImported

    External Data

    Dasty 3