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Protein

Probable acetate kinase

Gene

CNAG_06432

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway:iacetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable acetate kinase (CNAG_06432)
  2. no protein annotated in this organism
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121MagnesiumUniRule annotation
Binding sitei19 – 191ATPUniRule annotation
Binding sitei100 – 1001SubstrateUniRule annotation
Active sitei159 – 1591Proton donor/acceptorUniRule annotation
Sitei192 – 1921Transition state stabilizerUniRule annotation
Sitei253 – 2531Transition state stabilizerUniRule annotation
Metal bindingi416 – 4161MagnesiumUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2245ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.2.1. 1723.
UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
ORF Names:CNAG_06432Imported
OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)Imported
Taxonomic identifieri235443 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000010091 Componenti: Chromosome 13

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H0PX-ray1.89A/B1-430[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

OrthoDBiEOG7X9GH2.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase.
InterProiIPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

J9W3A6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDKAEYLLA INCGSSSIKG KLFAIPSFEL LANLAVTNIS SSDERVKIKT
60 70 80 90 100
TWEEGKGKDS EEEADYGDKI RYASLVPILL DHLTNSTHVK KEEIKYVCHR
110 120 130 140 150
VVHGGMHDKG IRVVKGHEEG LMEMDKLSEF APLHNHRAVL AVKSCIDALP
160 170 180 190 200
HHTSLLLFDT IFHRTIAPEV YTYALPPPDT ELTMPLRKYG FHGLSYASIV
210 220 230 240 250
QSLAEHLKKP SDQINVVVAH LGSGSSSCCI KNGKSIDTSM GLTPLEGLLG
260 270 280 290 300
GTRSGTIDPT AIFHHTEDAA SDANVGDFTV SKAEIILNKN SGFKALAGTT
310 320 330 340 350
NFGHIIQNLD PSKCSEEDHE KAKLTYAVFL DRLLNFVAQY LFKLLSEVPI
360 370 380 390 400
ESIDGLVFSG GIGEKGAELR RDVLKKLAWL GAEVDEEANN SNSGGAVKCI
410 420 430
TKEGSKLKGW VVETDEEGWM ARMAKEEFGF
Length:430
Mass (Da):47,039
Last modified:November 28, 2012 - v1
Checksum:iF2835D3B6D7C8B05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003832 Genomic DNA. Translation: AFR98670.1.
RefSeqiXP_012053491.1. XM_012198101.1.

Genome annotation databases

GeneIDi23889639.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003832 Genomic DNA. Translation: AFR98670.1.
RefSeqiXP_012053491.1. XM_012198101.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H0PX-ray1.89A/B1-430[»]
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23889639.

Phylogenomic databases

OrthoDBiEOG7X9GH2.

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.
BRENDAi2.7.2.1. 1723.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase.
InterProiIPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans."
    Thaker T.M., Tanabe M., Fowler M.L., Preininger A.M., Ingram-Smith C., Smith K.S., Iverson T.M.
    J. Struct. Biol. 181:185-189(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS).
  2. "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation."
    Janbon G., Ormerod K.L., Paulet D., Byrnes E.J.III., Yadav V., Chatterjee G., Mullapudi N., Hon C.C., Billmyre R.B., Brunel F., Bahn Y.S., Chen W., Chen Y., Chow E.W., Coppee J.Y., Floyd-Averette A., Gaillardin C., Gerik K.J.
    , Goldberg J., Gonzalez-Hilarion S., Gujja S., Hamlin J.L., Hsueh Y.P., Ianiri G., Jones S., Kodira C.D., Kozubowski L., Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K., Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A., Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A., Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A., Cuomo C.A., Dietrich F.S.
    PLoS Genet. 10:E1004261-E1004261(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487Imported.

Entry informationi

Entry nameiJ9W3A6_CRYNH
AccessioniPrimary (citable) accession number: J9W3A6
Entry historyi
Integrated into UniProtKB/TrEMBL: November 28, 2012
Last sequence update: November 28, 2012
Last modified: July 22, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.