Probable acetate kinase - Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Probable acetate kinase HAMAP-Rule MF_03131
EC=2.7.2.1 HAMAP-Rule MF_03131

Alternative name(s):
Acetokinase HAMAP-Rule MF_03131

Gene names

ORF Names:

CNAG_06432 EMBL AFR98670.1

Organism

Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) [Complete proteome] EMBL AFR98670.1

Taxonomic identifier

235443 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex ›

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + acetate = ADP + acetyl phosphate. HAMAP-Rule MF_03131
Cofactor	Mg²⁺ By similarity. HAMAP-Rule MF_03131
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. HAMAP-Rule MF_03131
Sequence similarities	Belongs to the acetokinase family. HAMAP-Rule MF_03131

Ontologies

Keywords
Ligand	ATP-binding HAMAP-Rule MF_03131 Magnesium HAMAP-Rule MF_03131 Metal-binding HAMAP-Rule MF_03131 Nucleotide-binding
Molecular function	Kinase HAMAP-Rule MF_03131 Transferase
Technical term	3D-structure PDB 4H0P Complete proteome
Gene Ontology (GO)
Biological_process	acetyl-CoA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway organic acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

220 – 224

5

ATP By similarity HAMAP-Rule MF_03131

Sites

Active site

159

1

Proton donor/acceptor By similarity HAMAP-Rule MF_03131

Metal binding

12

1

Magnesium By similarity HAMAP-Rule MF_03131

Metal binding

416

1

Magnesium By similarity HAMAP-Rule MF_03131

Binding site

19

1

ATP By similarity HAMAP-Rule MF_03131

Binding site

100

1

Substrate By similarity HAMAP-Rule MF_03131

Site

192

1

Transition state stabilizer By similarity HAMAP-Rule MF_03131

Site

253

1

Transition state stabilizer By similarity HAMAP-Rule MF_03131

Sequences

Sequence

Length

Mass (Da)

Tools

J9W3A6 [UniParc].

Last modified November 28, 2012. Version 1.
Checksum: F2835D3B6D7C8B05
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FASTA

430

47,039

        10         20         30         40         50         60 
MPDKAEYLLA INCGSSSIKG KLFAIPSFEL LANLAVTNIS SSDERVKIKT TWEEGKGKDS 

        70         80         90        100        110        120 
EEEADYGDKI RYASLVPILL DHLTNSTHVK KEEIKYVCHR VVHGGMHDKG IRVVKGHEEG 

       130        140        150        160        170        180 
LMEMDKLSEF APLHNHRAVL AVKSCIDALP HHTSLLLFDT IFHRTIAPEV YTYALPPPDT 

       190        200        210        220        230        240 
ELTMPLRKYG FHGLSYASIV QSLAEHLKKP SDQINVVVAH LGSGSSSCCI KNGKSIDTSM 

       250        260        270        280        290        300 
GLTPLEGLLG GTRSGTIDPT AIFHHTEDAA SDANVGDFTV SKAEIILNKN SGFKALAGTT 

       310        320        330        340        350        360 
NFGHIIQNLD PSKCSEEDHE KAKLTYAVFL DRLLNFVAQY LFKLLSEVPI ESIDGLVFSG 

       370        380        390        400        410        420 
GIGEKGAELR RDVLKKLAWL GAEVDEEANN SNSGGAVKCI TKEGSKLKGW VVETDEEGWM 

       430 
ARMAKEEFGF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Cryptococcus neoformans grubii H99." The Broad Institute Genome Sequencing Platform Birren B., Cuomo C., Gargeya S., Jaffe D., Young S.K., Wortman J., Zeng Q., Alvarado L., Dietrich F., Allen A., Stajich J.E., Heitman J., Kronstad J. Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
[2]	"Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans." Thaker T.M., Tanabe M., Fowler M.L., Preininger A.M., Ingram-Smith C., Smith K.S., Iverson T.M. J. Struct. Biol. 181:185-189(2013) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP003832 Genomic DNA. Translation: AFR98670.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4H0P	X-ray	1.89	A/B	1-430	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

UniPathway

UPA00340; UER00458.

Family and domain databases

HAMAP

MF_00020. Acetate_kinase.

InterPro

IPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]

PANTHER

PTHR21060. PTHR21060. 1 hit.

Pfam

PF00871. Acetate_kinase. 1 hit.
[Graphical view]

PRINTS

PR00471. ACETATEKNASE.

TIGRFAMs

TIGR00016. ackA. 1 hit.

PROSITE

PS01075. ACETATE_KINASE_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

J9W3A6_CRYNH

Accession

Primary (citable) accession number: J9W3A6

Entry history

Integrated into UniProtKB/TrEMBL:	November 28, 2012
Last sequence update:	November 28, 2012
Last modified:	May 1, 2013
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)