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J9W307 (J9W307_LACBU) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 10. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AFS00062.1
ORF Names:LBUCD034_1019 EMBL AFS00062.1
OrganismLactobacillus buchneri CD034 [Complete proteome] EMBL AFS00062.1
Taxonomic identifier1071400 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain142 – 348207ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding172 – 22756ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3021Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3171Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
J9W307 [UniParc].

Last modified November 28, 2012. Version 1.
Checksum: AE530920676FFC2E

FASTA37642,238
        10         20         30         40         50         60 
MEKHKKQHIG LIFGGNSSEH DVSKRSAHNI YDAMDKERYD VSLFLMTKNG YFLDHEASSR 

        70         80         90        100        110        120 
VFDGEPEDEV AKQELAKLDL KNPLARIINL AEESDIDVFF PIIHGNLGED GTIQGLLRLL 

       130        140        150        160        170        180 
HKPYVGTAIL GSAMSFDKDI TKKIVKLAGV PTTRYEVITP LTAEQYSYES LSDELGQTLF 

       190        200        210        220        230        240 
IKPANQGSSV GIHKVENAEE YSEGIRDAFR YDSKVLIEEA IEGPEELEIS ILGNDHPIAS 

       250        260        270        280        290        300 
KIGAIRVPKD DAFYTYDNKF VDASQVKFDV PVDISEELAQ QITEMGLTTY RALGLKGMAR 

       310        320        330        340        350        360 
IDYLVSKDDK PYMSEVNTLP GFTNISLYPQ LFAASGISYS ELIDRLIQLA IDEFERQSKI 

       370 
LYDFKPLPPR DQNINK 

« Hide

References

[1]"Insights into the completely annotated genome of Lactobacillus buchneri CD034, a strain isolated from stable grass silage."
Heinl S., Wibberg D., Eikmeyer F., Szczepanowski R., Blom J., Linke B., Goesmann A., Grabherr R., Schwab H., Puhler A., Schluter A.
J. Biotechnol. 161:153-166(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CD034 EMBL AFS00062.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003043 Genomic DNA. Translation: AFS00062.1.
RefSeqYP_006725644.1. NC_018610.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFS00062; AFS00062; LBUCD034_1019.
GeneID13655992.
KEGGlbn:LBUCD034_1019.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameJ9W307_LACBU
AccessionPrimary (citable) accession number: J9W307
Entry history
Integrated into UniProtKB/TrEMBL: November 28, 2012
Last sequence update: November 28, 2012
Last modified: June 11, 2014
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)