ID   J9W0K2_LENBU            Unreviewed;       502 AA.
AC   J9W0K2;
DT   28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT   28-NOV-2012, sequence version 1.
DT   24-JAN-2024, entry version 41.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|HAMAP-Rule:MF_01904};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_01904};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_01904};
DE            EC=4.1.1.31 {ECO:0000256|HAMAP-Rule:MF_01904};
GN   Name=ppcA {ECO:0000256|HAMAP-Rule:MF_01904,
GN   ECO:0000313|EMBL:AFR99933.1};
GN   ORFNames=LBUCD034_0886 {ECO:0000313|EMBL:AFR99933.1};
OS   Lentilactobacillus buchneri subsp. silagei CD034.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lentilactobacillus; Lentilactobacillus buchneri subsp. silagei.
OX   NCBI_TaxID=1071400 {ECO:0000313|EMBL:AFR99933.1, ECO:0000313|Proteomes:UP000007332};
RN   [1] {ECO:0000313|EMBL:AFR99933.1, ECO:0000313|Proteomes:UP000007332}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CD034 {ECO:0000313|EMBL:AFR99933.1,
RC   ECO:0000313|Proteomes:UP000007332};
RX   PubMed=22465289; DOI=10.1016/j.jbiotec.2012.03.007;
RA   Heinl S., Wibberg D., Eikmeyer F., Szczepanowski R., Blom J., Linke B.,
RA   Goesmann A., Grabherr R., Schwab H., Puhler A., Schluter A.;
RT   "Insights into the completely annotated genome of Lactobacillus buchneri
RT   CD034, a strain isolated from stable grass silage.";
RL   J. Biotechnol. 161:153-166(2012).
CC   -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC       phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC       dicarboxylic acid source for the tricarboxylic acid cycle.
CC       {ECO:0000256|HAMAP-Rule:MF_01904}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01904};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01904};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_01904}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000256|HAMAP-
CC       Rule:MF_01904}.
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DR   EMBL; CP003043; AFR99933.1; -; Genomic_DNA.
DR   RefSeq; WP_014939715.1; NC_018610.1.
DR   AlphaFoldDB; J9W0K2; -.
DR   STRING; 1071400.LBUCD034_0886; -.
DR   KEGG; lbn:LBUCD034_0886; -.
DR   PATRIC; fig|1071400.3.peg.843; -.
DR   eggNOG; COG1892; Bacteria.
DR   HOGENOM; CLU_517433_0_0_9; -.
DR   OrthoDB; 5487470at2; -.
DR   Proteomes; UP000007332; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_01904; PEPcase_type2; 1.
DR   InterPro; IPR007566; PEP_COase_arc-type.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   NCBIfam; TIGR02751; PEPCase_arch; 1.
DR   Pfam; PF14010; PEPcase_2; 1.
DR   PIRSF; PIRSF006677; UCP006677; 1.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|HAMAP-Rule:MF_01904};
KW   Lyase {ECO:0000256|HAMAP-Rule:MF_01904, ECO:0000313|EMBL:AFR99933.1};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_01904};
KW   Pyruvate {ECO:0000313|EMBL:AFR99933.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000007332}.
SQ   SEQUENCE   502 AA;  57236 MW;  EBCFE139984A717B CRC64;
     MDRKIPNIMG TQHPDNAGVP FFKHNDNAFV SAFREIDEAY QNFSKLDADE YMWDWEGKHA
     DASVIDRLYS EHYEYFQKHP LGKDKYLTFR LPNIWEEKGY SLMQAMTTIL SAEDFAYDLG
     FKERPLFEAI LPMTQSADQL ISMEDKFASL SHYKSTEFNT GHKDSDTLQM IPLFEGFESQ
     LHAPQILKKY LEMYKDHFGR ELSYMRVFLA GSDSALSNGF LNSIIGNKLA LTGLAEFEQE
     SGLPIYPIAG TGSTIFRGGL SPMMIDRYLE EFPGLKTATI QSAFRYDYPL DVVEPAIIKL
     RDGLQKSKFE IIDHDDQDIL LEIAKKAAAE YHETLDPLIN DLQPVFDAFP KRRDRHQHVG
     IIGYSRDVDG YKMPRAITFT GALYSVGVPP EFLGTGRILN KLSDKELNTL MKYYPNIKHD
     YAHVAKYFSK DALDALQAKN PAWKAVDEDI EAVERLFGIQ AGPQTPEQEK HAQLAADLVK
     ISDKETKTTL ISRQAILRKF LG
//