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Protein

Phosphoenolpyruvate carboxylase

Gene

ppcA

Organism
Lactobacillus buchneri CD034
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.UniRule annotation

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. phosphoenolpyruvate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbon fixation Source: UniProtKB-HAMAP
  2. oxaloacetate metabolic process Source: UniProtKB-HAMAP
  3. tricarboxylic acid cycle Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, PyruvateImported

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxylaseUniRule annotation (EC:4.1.1.31UniRule annotation)
Short name:
PEPCUniRule annotation
Short name:
PEPCaseUniRule annotation
Gene namesi
Name:ppcAUniRule annotationImported
ORF Names:LBUCD034_0886Imported
OrganismiLactobacillus buchneri CD034Imported
Taxonomic identifieri1071400 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000007332: Chromosome

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the PEPCase type 2 family.UniRule annotation

Phylogenomic databases

KOiK01595.

Family and domain databases

HAMAPiMF_01904. PEPcase_type2.
InterProiIPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF14010. PEPcase_2. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

J9W0K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRKIPNIMG TQHPDNAGVP FFKHNDNAFV SAFREIDEAY QNFSKLDADE
60 70 80 90 100
YMWDWEGKHA DASVIDRLYS EHYEYFQKHP LGKDKYLTFR LPNIWEEKGY
110 120 130 140 150
SLMQAMTTIL SAEDFAYDLG FKERPLFEAI LPMTQSADQL ISMEDKFASL
160 170 180 190 200
SHYKSTEFNT GHKDSDTLQM IPLFEGFESQ LHAPQILKKY LEMYKDHFGR
210 220 230 240 250
ELSYMRVFLA GSDSALSNGF LNSIIGNKLA LTGLAEFEQE SGLPIYPIAG
260 270 280 290 300
TGSTIFRGGL SPMMIDRYLE EFPGLKTATI QSAFRYDYPL DVVEPAIIKL
310 320 330 340 350
RDGLQKSKFE IIDHDDQDIL LEIAKKAAAE YHETLDPLIN DLQPVFDAFP
360 370 380 390 400
KRRDRHQHVG IIGYSRDVDG YKMPRAITFT GALYSVGVPP EFLGTGRILN
410 420 430 440 450
KLSDKELNTL MKYYPNIKHD YAHVAKYFSK DALDALQAKN PAWKAVDEDI
460 470 480 490 500
EAVERLFGIQ AGPQTPEQEK HAQLAADLVK ISDKETKTTL ISRQAILRKF

LG
Length:502
Mass (Da):57,236
Last modified:November 28, 2012 - v1
Checksum:iEBCFE139984A717B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003043 Genomic DNA. Translation: AFR99933.1.
RefSeqiYP_006725516.1. NC_018610.1.

Genome annotation databases

EnsemblBacteriaiAFR99933; AFR99933; LBUCD034_0886.
GeneIDi13655862.
KEGGilbn:LBUCD034_0886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003043 Genomic DNA. Translation: AFR99933.1.
RefSeqiYP_006725516.1. NC_018610.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFR99933; AFR99933; LBUCD034_0886.
GeneIDi13655862.
KEGGilbn:LBUCD034_0886.

Phylogenomic databases

KOiK01595.

Family and domain databases

HAMAPiMF_01904. PEPcase_type2.
InterProiIPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF14010. PEPcase_2. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Insights into the completely annotated genome of Lactobacillus buchneri CD034, a strain isolated from stable grass silage."
    Heinl S., Wibberg D., Eikmeyer F., Szczepanowski R., Blom J., Linke B., Goesmann A., Grabherr R., Schwab H., Puhler A., Schluter A.
    J. Biotechnol. 161:153-166(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CD034Imported.

Entry informationi

Entry nameiJ9W0K2_LACBU
AccessioniPrimary (citable) accession number: J9W0K2
Entry historyi
Integrated into UniProtKB/TrEMBL: November 28, 2012
Last sequence update: November 28, 2012
Last modified: January 7, 2015
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.