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J9U5U9 (DAD2_PETHY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 8. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable strigolactone esterase DAD2

Short name=3.1.-.-
Alternative name(s):
Protein DECREASED APICAL DOMINANCE 2
Gene names
Name:DAD2
OrganismPetunia hybrida (Petunia)
Taxonomic identifier4102 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in strigolactone signaling pathway. May function downstream of strigolactone synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form. Can hydrolyze the synthetic strigolactone analog GR24 in vitro. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds.

Subunit structure

Interacts with MAX2A in a strigolactone-depedent manner. Ref.1

Disruption phenotype

Increased shoot branching. Ref.1

Sequence similarities

Belongs to the AB hydrolase superfamily.

Ontologies

Keywords
   Molecular functionHydrolase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processsecondary shoot formation

Inferred from mutant phenotype Ref.1. Source: UniProtKB

strigolactone biosynthetic process

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionhydrolase activity, acting on ester bonds

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 267267Probable strigolactone esterase DAD2
PRO_0000422055

Sites

Active site961Nucleophile Probable
Active site2171 By similarity
Active site2461 Probable

Experimental info

Mutagenesis961S → A: Loss of activity and abolish interaction with MAX2A. Ref.1
Mutagenesis2461H → A: Loss of activity. Ref.1

Sequences

Sequence LengthMass (Da)Tools
J9U5U9 [UniParc].

Last modified November 28, 2012. Version 1.
Checksum: 49F4B5985C597C11

FASTA26729,712
        10         20         30         40         50         60 
MGQTLLDALN VRVVGSGERV LVLAHGFGTD QSAWNRILPF FLRDYRVVLY DLVCAGSVNP 

        70         80         90        100        110        120 
DFFDFRRYTT LDPYVDDLLH ILDALGIDCC AYVGHSVSAM IGILASIRRP ELFSKLILIG 

       130        140        150        160        170        180 
ASPRFLNDED YHGGFEQGEI EKVFSAMEAN YEAWVNGFAP LAVGADVPAA VREFSRTLFN 

       190        200        210        220        230        240 
MRPDITLFVS RTVFNSDMRG VLGLVKVPCH IFQTARDHSV PASVATYLKN HLGGKNTVHW 

       250        260 
LNIEGHLPHL SAPTLLAQEL RRALSHR 

« Hide

References

[1]"DAD2 is an alpha/beta hydrolase likely to be involved in the perception of the plant branching hormone, strigolactone."
Hamiaux C., Drummond R.S., Janssen B.J., Ledger S.E., Cooney J.M., Newcomb R.D., Snowden K.C.
Curr. Biol. 22:2032-2036(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), CATALYTIC ACTIVITY, INTERACTION WITH MAX2A, MUTAGENESIS OF SER-96 AND HIS-246, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
JQ654486 Genomic DNA. Translation: AFR68698.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4DNPX-ray2.15A1-267[»]
4DNQX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L1-267[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameDAD2_PETHY
AccessionPrimary (citable) accession number: J9U5U9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: November 28, 2012
Last modified: February 19, 2014
This is version 8 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references