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J9S678 (J9S678_9ACTO) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
ORF Names:KTR9_0338 EMBL AFR47004.1
OrganismGordonia sp. KTR9 [Complete proteome] EMBL AFR47004.1
Taxonomic identifier337191 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeGordoniaceaeGordonia

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain138 – 342205ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2951Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3091Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3091Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3111Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
J9S678 [UniParc].

Last modified November 28, 2012. Version 1.
Checksum: 204D34549DA335F2

FASTA35838,197
        10         20         30         40         50         60 
MSSRLLRLVV LYGGVSAEHD VSRVTAAHVL AAADRAKYDL VPIGIAKDGA WLRNDKAIAA 

        70         80         90        100        110        120 
IADGTPLPDT LEVAGTAVDP LTELRGHADE AITVVLPLLH GPHGEDGTIQ GMLELFKLPY 

       130        140        150        160        170        180 
VGAGVLSSAV CMDKAMAKIV AEQAGIPQCR WVEFRDGVDD PETVTARAID ALGLPVFVKP 

       190        200        210        220        230        240 
ANLGSSVGIT KAHDEAELDA AVNLALRYDN VVIIEEAVTG REIEVAVLGD TAPETSVPGE 

       250        260        270        280        290        300 
IKPGSEFYDY EDKYVTGAAQ LVIPAPVSDS AAAEVRELAA KTFTAMRCSG MARVDFFYEE 

       310        320        330        340        350 
SGRGWLLNEV NTIPGFTPGS MYPKLWEASG VSYPDLIDSL VTFALEVHRR RVGFSTEH 

« Hide

References

[1]"Genomic and transcriptomic studies of an RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine)-degrading actinobacterium."
Chen H.P., Zhu S.H., Casabon I., Hallam S.J., Crocker F.H., Mohn W.W., Indest K.J., Eltis L.D.
Appl. Environ. Microbiol. 78:7798-7800(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: KTR9 EMBL AFR47004.1.
[2]"Complete genome sequence of Gordonia sp. KTR9."
Chen H.-P., Lau A., Davis A.E., Zhu S., Eng S.W.M., Howes C.G., Crocker F.H., Indest K.J., Hallam S.J., Eltis L.D.
Appl. Environ. Microbiol. 0:0-0(2012)
Cited for: NUCLEOTIDE SEQUENCE.
Strain: KTR9 EMBL AFR47004.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002907 Genomic DNA. Translation: AFR47004.1.
RefSeqYP_006667140.1. NC_018581.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFR47004; AFR47004; KTR9_0338.
GeneID13568188.
KEGGgor:KTR9_0338.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameJ9S678_9ACTO
AccessionPrimary (citable) accession number: J9S678
Entry history
Integrated into UniProtKB/TrEMBL: November 28, 2012
Last sequence update: November 28, 2012
Last modified: June 11, 2014
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)