Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent 6-phosphofructokinase

Gene

LOC100163616

Organism
Acyrthosiphon pisum (Pea aphid)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261ATP; via amide nitrogenUniRule annotation
Metal bindingi120 – 1201Magnesium; catalyticUniRule annotation
Active sitei167 – 1671Proton acceptorUniRule annotation
Binding sitei202 – 2021Substrate; shared with dimeric partnerUniRule annotation
Binding sitei265 – 2651SubstrateUniRule annotation
Binding sitei293 – 2931Substrate; shared with dimeric partnerUniRule annotation
Binding sitei479 – 4791Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei574 – 5741Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei637 – 6371Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei663 – 6631Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei744 – 7441Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi89 – 902ATPUniRule annotation
Nucleotide bindingi119 – 1224ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:LOC100163616Imported
OrganismiAcyrthosiphon pisum (Pea aphid)Imported
Taxonomic identifieri7029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraSternorrhynchaAphidiformesAphidoideaAphididaeMacrosiphiniAcyrthosiphon
ProteomesiUP000007819 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 391391N-terminal catalytic PFK domain 1UniRule annotationAdd
BLAST
Regioni165 – 1673Substrate bindingUniRule annotation
Regioni209 – 2113Substrate bindingUniRule annotation
Regioni299 – 3024Substrate bindingUniRule annotation
Regioni392 – 40918Interdomain linkerUniRule annotationAdd
BLAST
Regioni410 – 788379C-terminal regulatory PFK domain 2UniRule annotationAdd
BLAST
Regioni536 – 5405Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni581 – 5833Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni669 – 6724Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

InParanoidiJ9JNX0.
KOiK00850.
OrthoDBiEOG7ZSHV5.
PhylomeDBiJ9JNX0.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

J9JNX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEDQKRFIE RGSHKGKGLA VFTSGGDSQG MNAAVRAVVR MAIYLGCKVF
60 70 80 90 100
FIKEGYQGMV DGGDNIEEAN WSSVSSIIHK GGTVIGSARC MDFKERVGRL
110 120 130 140 150
KAACNLVKRG ITNLVVIGGD GSLTGANLFR QEWSSLLDEL LQTSQINKAE
160 170 180 190 200
REKYKQLNIV GMVGSIDNDF CGTDMTIGTD SALHRIMDAI DAIVSTAYSH
210 220 230 240 250
QRTFIMEVMG RHCGYLALVT ALAAEADFVF IPEWPPHQDW ASKMCKKLLQ
260 270 280 290 300
ERTAGQRLNI IIVSEGAIDR DGQPITAEMV KQVVVDNLKQ DTRITVLGHV
310 320 330 340 350
QRGGAPSAFD RVLGCRMGAE AVMALMEATP ETEACVVSLD GNQAVRLPLM
360 370 380 390 400
ECVEKTKAVA KAMADKEWEL AVQLRGRSFA RNLETYKMLT RLKPPRSAFD
410 420 430 440 450
ELGRGLEGYT LAVMHIGAPA CGMNSAVRSF VRNCIYRGDT VYGIHDGVEG
460 470 480 490 500
LVAGNIQVMQ WSDVTGWVGQ GGAMLGTKRT LPEKRMPEIA ARLKEFNIQA
510 520 530 540 550
LLIIGGFEAY QAGIQLVQNR NNFPEFCIPM VIIPSTISNN VPGTEFSLGC
560 570 580 590 600
DTALNEITEI CDRIRQSAQG TKRRVFVIET MGGYCGYLAT VAGLAGGADA
610 620 630 640 650
AYIYEEKFTI KDLQNDVYHM ASKMAEGVQR GLILRNEKCS ENYNTDFIFR
660 670 680 690 700
LYTEEGKGLF STRMNVLGHM QQGGSPTPFD RNMGTKQAAK CVEWLVEKLR
710 720 730 740 750
ESTRPDGTIY TDNPDTAAMM GVIRRQYRFT PLTDLLPLTN FEQRIAKTQW
760 770 780
WLKLRPLLRI LAKHDSAYEE EGMYITVEEG LEADTLLA
Length:788
Mass (Da):87,112
Last modified:May 14, 2014 - v2
Checksum:iDE8B13663B384CBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABLF02041209 Genomic DNA. No translation available.
ABLF02041211 Genomic DNA. No translation available.
RefSeqiXP_003241124.1. XM_003241076.2.
UniGeneiAps.4611.
Aps.90631.

Genome annotation databases

EnsemblMetazoaiACYPI001940-RA; ACYPI001940-PA; ACYPI001940.
GeneIDi100160659.
KEGGiapi:100160659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABLF02041209 Genomic DNA. No translation available.
ABLF02041211 Genomic DNA. No translation available.
RefSeqiXP_003241124.1. XM_003241076.2.
UniGeneiAps.4611.
Aps.90631.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiACYPI001940-RA; ACYPI001940-PA; ACYPI001940.
GeneIDi100160659.
KEGGiapi:100160659.

Phylogenomic databases

InParanoidiJ9JNX0.
KOiK00850.
OrthoDBiEOG7ZSHV5.
PhylomeDBiJ9JNX0.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Jiang H., Abraham K., Ali S., Alsbrooks S.L., Anim B.N., Anosike U.S., Attaway T., Bandaranaike D.P., Battles P.K., Bell S.N., Bell A.V., Beltran B., Bickham C., Bustamante Y., Caleb T., Canada A., Cardenas V., Carter K.
    , Chacko J., Chandrabose M.N., Chavez D., Chavez A., Chen L., Chu H.-S., Claassen K.J., Cockrell R., Collins M., Cooper J.A., Cree A., Curry S.M., Da Y., Dao M.D., Das B., Davila M.-L., Davy-Carroll L., Denson S., Dinh H., Ebong V.E., Edwards J.R., Egan A., El-Daye J., Escobedo L., Fernandez S., Fernando P.R., Flagg N., Forbes L.D., Fowler R.G., Fu Q., Gabisi R.A., Ganer J., Garbino Pronczuk A., Garcia R.M., Garner T., Garrett T.E., Gonzalez D.A., Hamid H., Hawkins E.S., Hirani K., Hogues M.E., Hollins B., Hsiao C.-H., Jabil R., James M.L., Jhangiani S.N., Johnson B., Johnson Q., Joshi V., Kalu J.B., Kam C., Kashfia A., Keebler J., Kisamo H., Kovar C.L., Lago L.A., Lai C.-Y., Laidlaw J., Lara F., Le T.-K., Lee S.L., Legall F.H., Lemon S.J., Lewis L.R., Li B., Liu Y., Liu Y.-S., Lopez J., Lozado R.J., Lu J., Madu R.C., Maheshwari M., Maheshwari R., Malloy K., Martinez E., Mathew T., Mercado I.C., Mercado C., Meyer B., Montgomery K., Morgan M.B., Munidasa M., Nazareth L.V., Nelson J., Ng B.M., Nguyen N.B., Nguyen P.Q., Nguyen T., Obregon M., Okwuonu G.O., Onwere C.G., Orozco G., Parra A., Patel S., Patil S., Perez A., Perez Y., Pham C., Primus E.L., Pu L.-L., Puazo M., Qin X., Quiroz J.B., Reese J., Richards S., Rives C.M., Robberts R., Ruiz S.J., Ruiz M.J., Santibanez J., Schneider B.W., Sisson I., Smith M., Sodergren E., Song X.-Z., Song B.B., Summersgill H., Thelus R., Thornton R.D., Trejos Z.Y., Usmani K., Vattathil S., Villasana D., Walker D.L., Wang S., Wang K., White C.S., Williams A.C., Williamson J., Wilson K., Woghiren I.O., Woodworth J.R., Worley K.C., Wright R.A., Wu W., Young L., Zhang L., Zhang J., Zhu Y., Muzny D.M., Weinstock G., Gibbs R.A.
    Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. EnsemblMetazoa
    Submitted (FEB-2015) to UniProtKB
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiJ9JNX0_ACYPI
AccessioniPrimary (citable) accession number: J9JNX0
Entry historyi
Integrated into UniProtKB/TrEMBL: October 31, 2012
Last sequence update: May 14, 2014
Last modified: May 27, 2015
This is version 23 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.