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Protein

ATP-dependent 6-phosphofructokinase

Gene

LOC100160659

Organism
Acyrthosiphon pisum (Pea aphid)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (LOC100161161)
  3. ATP-dependent 6-phosphofructokinase (LOC100160659)
  4. Fructose-bisphosphate aldolase (LOC100166128)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26ATP; via amide nitrogenUniRule annotation1
Metal bindingi120Magnesium; catalyticUniRule annotation1
Active sitei167Proton acceptorUniRule annotation1
Binding sitei202Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei265SubstrateUniRule annotation1
Binding sitei293Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei479Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei574Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei637Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei663Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei744Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi89 – 90ATPUniRule annotation2
Nucleotide bindingi119 – 122ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzymeUniRule annotation, KinaseUniRule annotation, Transferase
Biological processGlycolysisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:LOC100160659Imported
OrganismiAcyrthosiphon pisum (Pea aphid)Imported
Taxonomic identifieri7029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraSternorrhynchaAphidomorphaAphidoideaAphididaeMacrosiphiniAcyrthosiphon
Proteomesi
  • UP000007819 Componenti: Unassembled WGS sequence

Subcellular locationi

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi7029.ACYPI001940-PA

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 324PFKInterPro annotationAdd BLAST306
Domaini411 – 695PFKInterPro annotationAdd BLAST285

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 391N-terminal catalytic PFK domain 1UniRule annotationAdd BLAST391
Regioni165 – 167Substrate bindingUniRule annotation3
Regioni209 – 211Substrate bindingUniRule annotation3
Regioni299 – 302Substrate bindingUniRule annotation4
Regioni410 – 788C-terminal regulatory PFK domain 2UniRule annotationAdd BLAST379
Regioni536 – 540Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
Regioni581 – 583Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
Regioni669 – 672Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440 Eukaryota
COG0205 LUCA
InParanoidiJ9JNX0
PhylomeDBiJ9JNX0

Family and domain databases

HAMAPiMF_03184 Phosphofructokinase_I_E, 1 hit
InterProiView protein in InterPro
IPR009161 6-Pfructokinase_euk
IPR022953 ATP_PFK
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 2 hits
PIRSFiPIRSF000533 ATP_PFK_euk, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 2 hits
TIGRFAMsiTIGR02478 6PF1K_euk, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 2 hits

Sequencei

Sequence statusi: Complete.

J9JNX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEDQKRFIE RGSHKGKGLA VFTSGGDSQG MNAAVRAVVR MAIYLGCKVF
60 70 80 90 100
FIKEGYQGMV DGGDNIEEAN WSSVSSIIHK GGTVIGSARC MDFKERVGRL
110 120 130 140 150
KAACNLVKRG ITNLVVIGGD GSLTGANLFR QEWSSLLDEL LQTSQINKAE
160 170 180 190 200
REKYKQLNIV GMVGSIDNDF CGTDMTIGTD SALHRIMDAI DAIVSTAYSH
210 220 230 240 250
QRTFIMEVMG RHCGYLALVT ALAAEADFVF IPEWPPHQDW ASKMCKKLLQ
260 270 280 290 300
ERTAGQRLNI IIVSEGAIDR DGQPITAEMV KQVVVDNLKQ DTRITVLGHV
310 320 330 340 350
QRGGAPSAFD RVLGCRMGAE AVMALMEATP ETEACVVSLD GNQAVRLPLM
360 370 380 390 400
ECVEKTKAVA KAMADKEWEL AVQLRGRSFA RNLETYKMLT RLKPPRSAFD
410 420 430 440 450
ELGRGLEGYT LAVMHIGAPA CGMNSAVRSF VRNCIYRGDT VYGIHDGVEG
460 470 480 490 500
LVAGNIQVMQ WSDVTGWVGQ GGAMLGTKRT LPEKRMPEIA ARLKEFNIQA
510 520 530 540 550
LLIIGGFEAY QAGIQLVQNR NNFPEFCIPM VIIPSTISNN VPGTEFSLGC
560 570 580 590 600
DTALNEITEI CDRIRQSAQG TKRRVFVIET MGGYCGYLAT VAGLAGGADA
610 620 630 640 650
AYIYEEKFTI KDLQNDVYHM ASKMAEGVQR GLILRNEKCS ENYNTDFIFR
660 670 680 690 700
LYTEEGKGLF STRMNVLGHM QQGGSPTPFD RNMGTKQAAK CVEWLVEKLR
710 720 730 740 750
ESTRPDGTIY TDNPDTAAMM GVIRRQYRFT PLTDLLPLTN FEQRIAKTQW
760 770 780
WLKLRPLLRI LAKHDSAYEE EGMYITVEEG LEADTLLA
Length:788
Mass (Da):87,112
Last modified:May 14, 2014 - v2
Checksum:iDE8B13663B384CBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABLF02041209 Genomic DNA No translation available.
ABLF02041211 Genomic DNA No translation available.
RefSeqiXP_003241124.1, XM_003241076.3
UniGeneiAps.29014
Aps.4611
Aps.90631

Genome annotation databases

EnsemblMetazoaiACYPI001940-RA; ACYPI001940-PA; ACYPI001940
GeneIDi100160659

Similar proteinsi

Entry informationi

Entry nameiJ9JNX0_ACYPI
AccessioniPrimary (citable) accession number: J9JNX0
Entry historyiIntegrated into UniProtKB/TrEMBL: October 31, 2012
Last sequence update: May 14, 2014
Last modified: March 28, 2018
This is version 41 of the entry and version 2 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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