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D-alanine--D-alanine ligase



Escherichia coli
Unreviewed-Annotation score: -Protein inferred from homologyi


Cell wall formation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation


Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation
  • Mn2+SAAS annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.


Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi257Magnesium or manganese 1UniRule annotation1
Metal bindingi270Magnesium or manganese 1UniRule annotation1
Metal bindingi270Magnesium or manganese 2UniRule annotation1
Metal bindingi272Magnesium or manganese 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi


Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processCell cycle, Cell divisionImported, Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandATP-bindingPROSITE-ProRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases


Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotationSAAS annotation (EC: annotationSAAS annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Synonyms:ddlUniRule annotationImported
ORF Names:A8C65_16265Imported, A9R57_20470Imported, AC789_1c00920Imported, ACN002_0092Imported, ACU57_16065Imported, AKN40_4149Imported, AM266_01485Imported, AM270_12055Imported, AM333_09520Imported, AM341_08845Imported, AMK83_01600Imported, AML07_23605Imported, APT94_27415Imported, APZ14_15185Imported, AUQ13_07885Imported, AUS26_14655Imported, AW059_09405Imported, AWE18_25425Imported, B1K96_13400Imported, BB545_20490Imported, BE963_14430Imported, BET08_06765Imported, BHF46_08275Imported, BHS87_00390Imported, BIQ87_00385Imported, BIU72_13840Imported, BJJ90_21750Imported, BK248_21020Imported, BK292_13285Imported, BK334_15405Imported, BK373_09210Imported, BK400_03995Imported, BMR12_10430Imported, BMR18_17245Imported, BMR21_15460Imported, BMR28_20785Imported, BMR35_12605Imported, BMR49_06505Imported, BMT53_09900Imported, BMT94_05365Imported, BN17_45141Imported, BUE81_03720Imported, BVL39_02595Imported, BW690_22025Imported, BXT93_15680Imported, BZL31_13400Imported, CA268_07855Imported, CA593_03280Imported, CDL49_15860Imported, CES94_21230Imported, CF006_13845Imported, CGZ52_01865Imported, CIJ94_16040Imported, COD46_06225Imported, CR534_22775Imported, CR538_21050Imported, CR539_04520Imported, CRN68_14465Imported, CSB64_19500Imported, CSB65_15085Imported, CTI76_04810Imported, CU078_10955Imported, CWM28_26875Imported, EC3234A_2c00760Imported, ECONIH1_00510Imported, EL75_3670Imported, EL79_3780Imported, EL80_3727Imported, ERS085365_01030Imported, ERS085379_00613Imported, ERS085416_01582Imported, ERS139211_00749Imported, ERS150876_00142Imported, FORC28_5107Imported, GJ11_00490Imported, HMPREF3040_02529Imported, HW43_04190Imported, JD73_13510Imported, MJ49_06580Imported, MS6198_01060Imported, PGD_03733Imported, PU06_15025Imported, RX35_04829Imported, SK85_00090Imported, SM09_00836Imported, UN86_27885Imported, UN91_10970Imported, WQ89_09170Imported, WR15_01945Imported
OrganismiEscherichia coliImported
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
  • UP000044893 Componenti: Unassembled WGS sequence
  • UP000230908 Componenti: Chromosome
  • UP000232093 Componenti: Unassembled WGS sequence
  • UP000225895 Componenti: Unassembled WGS sequence
  • UP000217968 Componenti: Unassembled WGS sequence
  • UP000229255 Componenti: Unassembled WGS sequence
  • UP000193530 Componenti: Unassembled WGS sequence
  • UP000194908 Componenti: Unassembled WGS sequence
  • UP000030018 Componenti: Unassembled WGS sequence
  • UP000030000 Componenti: Unassembled WGS sequence
  • UP000065978 Componenti: Chromosome
  • UP000197281 Componenti: Unassembled WGS sequence
  • UP000194247 Componenti: Unassembled WGS sequence
  • UP000188724 Componenti: Unassembled WGS sequence
  • UP000070045 Componenti: Unassembled WGS sequence
  • UP000234324 Componenti: Chromosome
  • UP000037564 Componenti: Unassembled WGS sequence
  • UP000217192 Componenti: Unassembled WGS sequence
  • UP000051846 Componenti: Unassembled WGS sequence
  • UP000094504 Componenti: Chromosome
  • UP000215625 Componenti: Unassembled WGS sequence
  • UP000194285 Componenti: Unassembled WGS sequence
  • UP000040934 Componenti: Unassembled WGS sequence
  • UP000070450 Componenti: Unassembled WGS sequence
  • UP000234238 Componenti: Chromosome
  • UP000225370 Componenti: Unassembled WGS sequence
  • UP000185422 Componenti: Chromosome
  • UP000185961 Componenti: Unassembled WGS sequence
  • UP000044469 Componenti: Unassembled WGS sequence
  • UP000222984 Componenti: Unassembled WGS sequence
  • UP000036012 Componenti: Unassembled WGS sequence
  • UP000230960 Componenti: Chromosome
  • UP000028651 Componenti: Unassembled WGS sequence
  • UP000229954 Componenti: Unassembled WGS sequence
  • UP000033371 Componenti: Unassembled WGS sequence
  • UP000186204 Componenti: Unassembled WGS sequence
  • UP000188465 Componenti: Unassembled WGS sequence
  • UP000223505 Componenti: Chromosome
  • UP000031522 Componenti: Chromosome
  • UP000029988 Componenti: Unassembled WGS sequence
  • UP000030783 Componenti: Chromosome
  • UP000230043 Componenti: Chromosome
  • UP000054864 Componenti: Unassembled WGS sequence
  • UP000077380 Componenti: Unassembled WGS sequence
  • UP000036812 Componenti: Unassembled WGS sequence
  • UP000030834 Componenti: Unassembled WGS sequence
  • UP000194331 Componenti: Chromosome
  • UP000095493 Componenti: Unassembled WGS sequence
  • UP000184168 Componenti: Unassembled WGS sequence
  • UP000218721 Componenti: Unassembled WGS sequence
  • UP000054277 Componenti: Unassembled WGS sequence
  • UP000215493 Componenti: Unassembled WGS sequence
  • UP000197584 Componenti: Chromosome
  • UP000184369 Componenti: Unassembled WGS sequence
  • UP000203621 Componenti: Chromosome
  • UP000066343 Componenti: Chromosome
  • UP000188758 Componenti: Unassembled WGS sequence
  • UP000030317 Componenti: Chromosome
  • UP000036405 Componenti: Unassembled WGS sequence
  • UP000076262 Componenti: Unassembled WGS sequence
  • UP000040589 Componenti: Unassembled WGS sequence
  • UP000233539 Componenti: Unassembled WGS sequence
  • UP000033592 Componenti: Unassembled WGS sequence
  • UP000224850 Componenti: Unassembled WGS sequence
  • UP000234543 Componenti: Chromosome
  • UP000222575 Componenti: Unassembled WGS sequence
  • UP000184459 Componenti: Unassembled WGS sequence
  • UP000094022 Componenti: Unassembled WGS sequence
  • UP000094451 Componenti: Chromosome
  • UP000184336 Componenti: Unassembled WGS sequence
  • UP000234906 Componenti: Chromosome
  • UP000051850 Componenti: Unassembled WGS sequence
  • UP000189664 Componenti: Chromosome
  • UP000092566 Componenti: Chromosome
  • UP000184178 Componenti: Unassembled WGS sequence
  • UP000225496 Componenti: Unassembled WGS sequence
  • UP000050556 Componenti: Unassembled WGS sequence
  • UP000222175 Componenti: Unassembled WGS sequence
  • UP000196822 Componenti: Unassembled WGS sequence
  • UP000076304 Componenti: Unassembled WGS sequence
  • UP000095183 Componenti: Unassembled WGS sequence
  • UP000214661 Componenti: Unassembled WGS sequence
  • UP000194199 Componenti: Chromosome
  • UP000188967 Componenti: Unassembled WGS sequence
  • UP000041959 Componenti: Unassembled WGS sequence
  • UP000070338 Componenti: Unassembled WGS sequence
  • UP000216687 Componenti: Unassembled WGS sequence
  • UP000225731 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation


Protein-protein interaction databases


Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 303ATP-graspInterPro annotationAdd BLAST203

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CPF. Bacteria.
COG1181. LUCA.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom_sf.
PfamiView protein in Pfam
PF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFiPIRSF039102. Ddl/VanB. 2 hits.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.


Sequence statusi: Complete.

J7QWX0-1 [UniParc]FASTAAdd to basket

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260 270 280 290 300

Mass (Da):32,840
Last modified:October 31, 2012 - v1

Sequence databases

Select the link destinations:
Links Updated
CP009859 Genomic DNA. Translation: AIX61817.1.
CP009106 Genomic DNA. Translation: AIZ85670.1.
CP010315 Genomic DNA. Translation: AJE54391.1.
CP013025 Genomic DNA. Translation: ALL87292.1.
CP007491 Genomic DNA. Translation: ALY11550.1.
CP015229 Genomic DNA. Translation: ANO87245.1.
CP012693 Genomic DNA. Translation: AOM48083.1.
CP015834 Genomic DNA. Translation: AOM68394.1.
CP017631 Genomic DNA. Translation: APT04441.1.
CP018957 Genomic DNA. Translation: AQV30681.1.
CP021175 Genomic DNA. Translation: ARR32215.1.
CP021202 Genomic DNA. Translation: ARR59340.1.
CP022154 Genomic DNA. Translation: ASG51191.1.
CP012378 Genomic DNA. Translation: ASO80915.1.
CP023960 Genomic DNA. Translation: ATM81914.1.
CP024821 Genomic DNA. Translation: ATX09207.1.
CP024851 Genomic DNA. Translation: ATX41938.1.
CP024859 Genomic DNA. Translation: ATX51873.1.
CP024147 Genomic DNA. Translation: AUJ94752.1.
CP024141 Genomic DNA. Translation: AUK02699.1.
CP024127 Genomic DNA. Translation: AUK23634.1.
CP019051 Genomic DNA. Translation: AUL67125.1.
HE964769 Genomic DNA. Translation: CCJ42560.1.
HG976958 Genomic DNA. Translation: CDU40703.1.
CYAQ01000006 Genomic DNA. Translation: CTS13247.1.
CYBF01000003 Genomic DNA. Translation: CTT31890.1.
CYDH01000006 Genomic DNA. Translation: CTV24037.1.
CYCX01000010 Genomic DNA. Translation: CTV54797.1.
CYGC01000001 Genomic DNA. Translation: CTZ22945.1.
JPQG01000002 Genomic DNA. Translation: KFD77273.1.
JPKH01000046 Genomic DNA. Translation: KGM67917.1.
JPKL01000117 Genomic DNA. Translation: KGM77191.1.
JPMZ01000050 Genomic DNA. Translation: KGM79834.1.
JUDN01000073 Genomic DNA. Translation: KHJ17794.1.
JZDU01000179 Genomic DNA. Translation: KJW22478.1.
JZDZ01000075 Genomic DNA. Translation: KJW50286.1.
LEUM01000001 Genomic DNA. Translation: KLY04786.1.
LEYN01000010 Genomic DNA. Translation: KME75430.1.
LGZN01000008 Genomic DNA. Translation: KNF71902.1.
LGZP01000016 Genomic DNA. Translation: KNF80630.1.
LDYI01000114 Genomic DNA. Translation: KPO09899.1.
LIVE01000002 Genomic DNA. Translation: KQJ19130.1.
LIVI01000012 Genomic DNA. Translation: KQJ42204.1.
LMBK01000498 Genomic DNA. Translation: KST28507.1.
LNHR01000091 Genomic DNA. Translation: KSX94159.1.
LSNZ01000086 Genomic DNA. Translation: KXG98721.1.
LPXZ01000183 Genomic DNA. Translation: KXL60979.1.
LOOZ01000011 Genomic DNA. Translation: KXR92089.1.
LOFS01000184 Genomic DNA. Translation: KYR47176.1.
LOCS01000187 Genomic DNA. Translation: KYV63191.1.
LCVH01000002 Genomic DNA. Translation: OAC45378.1.
LZEH01000266 Genomic DNA. Translation: OEI58961.1.
MIWY01000006 Genomic DNA. Translation: OEN36044.1.
MOFD01000048 Genomic DNA. Translation: OJK73558.1.
MOGU01000021 Genomic DNA. Translation: OJM94989.1.
MOIL01000015 Genomic DNA. Translation: OJP12822.1.
MOJX01000010 Genomic DNA. Translation: OJR05340.1.
MOKZ01000004 Genomic DNA. Translation: OJS90695.1.
MPGT01000001 Genomic DNA. Translation: OKB89803.1.
MSEK01000030 Genomic DNA. Translation: OLR88733.1.
MTKH01000187 Genomic DNA. Translation: ONG25915.1.
MTPS01000255 Genomic DNA. Translation: ONG33926.1.
MCNL01000379 Genomic DNA. Translation: ONK54063.1.
MOZG01000005 Genomic DNA. Translation: OOJ09181.1.
NAFT01000003 Genomic DNA. Translation: ORT10577.1.
NEME01000004 Genomic DNA. Translation: OTB40202.1.
NBCM01000016 Genomic DNA. Translation: OTE92569.1.
MUAB01000024 Genomic DNA. Translation: OUK50841.1.
LYBG01000819 Genomic DNA. Translation: OWD67099.1.
NACG01000001 Genomic DNA. Translation: OWX87637.1.
NIIE01000016 Genomic DNA. Translation: OXJ80307.1.
LEOO01000028 Genomic DNA. Translation: OYC64409.1.
NKYK01000012 Genomic DNA. Translation: OZM91570.1.
NQIT01000047 Genomic DNA. Translation: OZX93798.1.
MVPO01000026 Genomic DNA. Translation: PAR03148.1.
NWAO01000006 Genomic DNA. Translation: PBR53162.1.
NTEV01000146 Genomic DNA. Translation: PBU42717.1.
PCGF01000082 Genomic DNA. Translation: PDO56200.1.
MPHW01000036 Genomic DNA. Translation: PGF63886.1.
MPHZ01000025 Genomic DNA. Translation: PGF81501.1.
MPIJ01000036 Genomic DNA. Translation: PGG19508.1.
MOXU01000015 Genomic DNA. Translation: PGG40139.1.
NXNT01000035 Genomic DNA. Translation: PHL35646.1.
NXOH01000006 Genomic DNA. Translation: PHL46220.1.
PEDR01000026 Genomic DNA. Translation: PHW95389.1.
PEDQ01000044 Genomic DNA. Translation: PHW99629.1.
PEQI01000036 Genomic DNA. Translation: PIM59284.1.
PIOU01000191 Genomic DNA. Translation: PJY16346.1.
NMUP01000003 Genomic DNA. Translation: PKR65171.1.
RefSeqiWP_000130056.1. NZ_PJIY01000009.1.

Genome annotation databases

EnsemblBacteriaiAIX61817; AIX61817; ECONIH1_00510.
AJE54391; AJE54391; AC789_1c00920.
ALL87292; ALL87292; MJ49_06580.
ALY11550; ALY11550; ACN002_0092.
ALZ67075; ALZ67075; AWH59_13345.
ANM85311; ANM85311; A8V37_23525.
ANO87245; ANO87245; GJ11_00490.
ANP16212; ANP16212; GJ12_00490.
AOM48083; AOM48083; FORC28_5107.
AOM68394; AOM68394; MS6198_01060.
CDU40703; CDU40703; PGD_03733.
CTS13247; CTS13247; ERS085365_01030.
CTT31890; CTT31890; ERS085379_00613.
CTV24037; CTV24037; ERS139211_00749.
CTV54797; CTV54797; ERS085416_01582.
CTX15751; CTX15751; ERS139235_01279.
CTY13197; CTY13197; ERS139266_02404.
CTZ22945; CTZ22945; ERS150876_00142.
KFD77273; KFD77273; JD73_13510.
KGM67917; KGM67917; EL75_3670.
KGM77191; KGM77191; EL80_3727.
KGM79834; KGM79834; EL79_3780.
KHH07020; KHH07020; PU69_20425.
KHJ17794; KHJ17794; PU06_15025.
KIG81022; KIG81022; PU38_12125.
KIH05999; KIH05999; PU21_08070.
KIH34994; KIH34994; PD07_16595.
KIQ46899; KIQ46899; IY32_08180.
KJW22478; KJW22478; UN86_27885.
KJW50286; KJW50286; UN91_10970.
KLX57401; KLX57401; SK78_02604.
KLX88225; KLX88225; SK83_02692.
KLY04786; KLY04786; SK85_00090.
KME75430; KME75430; SM09_00836.
KNF71902; KNF71902; WR15_01945.
KNF80630; KNF80630; WQ89_09170.
KNY77441; KNY77441; AGA26_05895.
KPO09899; KPO09899; ACU57_16065.
KPO35985; KPO35985; ACU81_18180.
KQJ19130; KQJ19130; AM266_01485.
KQJ42204; KQJ42204; AM270_12055.
KST28507; KST28507; APZ14_15185.
KSX94159; KSX94159; APT94_27415.
KXG98721; KXG98721; HMPREF3040_02529.
KXL60979; KXL60979; AUS26_14655.
KXQ36579; KXQ36579; AUQ01_21005.
KYR47176; KYR47176; AML07_23605.
KYV63191; KYV63191; AMK83_01600.
KZJ55379; KZJ55379; AWG90_06655.
OAC45378; OAC45378; EC3234A_2c00760.
OAJ86221; OAJ86221; A5958_05810.
OAO70539; OAO70539; OO96_05110.
OAS92324; OAS92324; A6I92_00685.
OCO32447; OCO32447; AN669_0200600.
ODA84481; ODA84481; A9D65_12690.
ODH24296; ODH24296; A6804_06060.
ODH28795; ODH28795; A6803_16340.
OEI58961; OEI58961; A9R57_20470.
OEN36044; OEN36044; BHF46_08275.

Similar proteinsi

Entry informationi

Entry nameiJ7QWX0_ECOLX
AccessioniPrimary (citable) accession number: J7QWX0
Secondary accession number(s): A0A0J3ZAN0
Entry historyiIntegrated into UniProtKB/TrEMBL: October 31, 2012
Last sequence update: October 31, 2012
Last modified: March 28, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)


Keywords - Technical termi

Complete proteomeImported