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J7QFI5

- J7QFI5_METSZ

UniProt

J7QFI5 - J7QFI5_METSZ

Protein

Isocitrate dehydrogenase [NADP]

Gene

BN69_0895

Organism
Methylocystis sp. (strain SC2)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 12 (01 Oct 2014)
      Sequence version 1 (31 Oct 2012)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei77 – 771SubstrateUniRule annotation
    Binding sitei82 – 821NADP
    Binding sitei109 – 1091SubstrateUniRule annotation
    Binding sitei132 – 1321SubstrateUniRule annotation
    Sitei139 – 1391Critical for catalysisUniRule annotation
    Sitei210 – 2101Critical for catalysisUniRule annotation
    Metal bindingi250 – 2501Magnesium or manganeseUniRule annotation
    Binding sitei258 – 2581NADP
    Metal bindingi273 – 2731Magnesium or manganeseUniRule annotation
    Binding sitei326 – 3261NADP; via amide nitrogen and carbonyl oxygen

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 773NADP
    Nucleotide bindingi308 – 3136NADP

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotationImported

    Keywords - Biological processi

    Tricarboxylic acid cycleUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
    Gene namesi
    Ordered Locus Names:BN69_0895Imported
    OrganismiMethylocystis sp. (strain SC2)Imported
    Taxonomic identifieri187303 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylocystaceaeMethylocystis
    ProteomesiUP000005263: Chromosome

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni94 – 1007Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

    Phylogenomic databases

    KOiK00031.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    J7QFI5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKIKVASPV VELDGDEMTR IIWAAIKEKL IRPYLDVDLL YYDLSIQNRD    50
    ATNDQVTIDA AYAIKKHGVG VKCATITPDE ARVKEFNLKE MWKSPNGTIR 100
    NILGGVIFRE PIICRNVPRL VPGWTQPIIV GRHAFGDQYK ATDFKVPGKG 150
    RLTIKFEGVD GDVIEKEVFN FPGAGVALAM YNLDESISEF ARATFNYGLT 200
    RRYPVYLSTK NTILKAYDGR FKDLFQEIYD AEFKSKFEAL GLTYEHRLID 250
    DMVASALKWS GGYIWACKNY DGDVQSDTVA QGFGSLGLMT SVLMTPDGKT 300
    VEAEAAHGTV TRHYREHQKG HETSTNSIAS IFAWTRALAH RGKLDGNDEL 350
    TRFARTLEDV CVATVESGFM TKDLALLVGC NQKWLSTTGF LDKIDENLRR 400
    AME 403
    Length:403
    Mass (Da):45,252
    Last modified:October 31, 2012 - v1
    Checksum:iE28F4A135E81B681
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HE956757 Genomic DNA. Translation: CCJ06346.1.
    RefSeqiWP_014890380.1. NC_018485.1.
    YP_006590997.1. NC_018485.1.

    Genome annotation databases

    EnsemblBacteriaiCCJ06346; CCJ06346; BN69_0895.
    GeneIDi13519097.
    KEGGimsc:BN69_0895.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HE956757 Genomic DNA. Translation: CCJ06346.1 .
    RefSeqi WP_014890380.1. NC_018485.1.
    YP_006590997.1. NC_018485.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CCJ06346 ; CCJ06346 ; BN69_0895 .
    GeneIDi 13519097.
    KEGGi msc:BN69_0895.

    Phylogenomic databases

    KOi K00031.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Methylocystis sp. strain SC2, an aerobic methanotroph with high-affinity methane oxidation potential."
      Dam B., Dam S., Kube M., Reinhardt R., Liesack W.
      J. Bacteriol. 194:6008-6009(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC2Imported.

    Entry informationi

    Entry nameiJ7QFI5_METSZ
    AccessioniPrimary (citable) accession number: J7QFI5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 31, 2012
    Last sequence update: October 31, 2012
    Last modified: October 1, 2014
    This is version 12 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3