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Protein

Isocitrate dehydrogenase [NADP]

Gene

BN69_0895

Organism
Methylocystis sp. (strain SC2)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771SubstrateUniRule annotation
Binding sitei82 – 821NADPUniRule annotation
Binding sitei109 – 1091SubstrateUniRule annotation
Binding sitei132 – 1321SubstrateUniRule annotation
Sitei139 – 1391Critical for catalysisUniRule annotation
Sitei210 – 2101Critical for catalysisUniRule annotation
Metal bindingi250 – 2501Magnesium or manganeseUniRule annotation
Binding sitei258 – 2581NADPUniRule annotation
Metal bindingi273 – 2731Magnesium or manganeseUniRule annotation
Binding sitei326 – 3261NADP; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 773NADPUniRule annotation
Nucleotide bindingi308 – 3136NADPUniRule annotation

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. isocitrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Ordered Locus Names:BN69_0895Imported
OrganismiMethylocystis sp. (strain SC2)Imported
Taxonomic identifieri187303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylocystaceaeMethylocystis
ProteomesiUP000005263: Chromosome

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 1007Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

KOiK00031.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

J7QFI5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRKIKVASPV VELDGDEMTR IIWAAIKEKL IRPYLDVDLL YYDLSIQNRD
60 70 80 90 100
ATNDQVTIDA AYAIKKHGVG VKCATITPDE ARVKEFNLKE MWKSPNGTIR
110 120 130 140 150
NILGGVIFRE PIICRNVPRL VPGWTQPIIV GRHAFGDQYK ATDFKVPGKG
160 170 180 190 200
RLTIKFEGVD GDVIEKEVFN FPGAGVALAM YNLDESISEF ARATFNYGLT
210 220 230 240 250
RRYPVYLSTK NTILKAYDGR FKDLFQEIYD AEFKSKFEAL GLTYEHRLID
260 270 280 290 300
DMVASALKWS GGYIWACKNY DGDVQSDTVA QGFGSLGLMT SVLMTPDGKT
310 320 330 340 350
VEAEAAHGTV TRHYREHQKG HETSTNSIAS IFAWTRALAH RGKLDGNDEL
360 370 380 390 400
TRFARTLEDV CVATVESGFM TKDLALLVGC NQKWLSTTGF LDKIDENLRR

AME
Length:403
Mass (Da):45,252
Last modified:October 31, 2012 - v1
Checksum:iE28F4A135E81B681
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE956757 Genomic DNA. Translation: CCJ06346.1.
RefSeqiWP_014890380.1. NC_018485.1.
YP_006590997.1. NC_018485.1.

Genome annotation databases

EnsemblBacteriaiCCJ06346; CCJ06346; BN69_0895.
GeneIDi13519097.
KEGGimsc:BN69_0895.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE956757 Genomic DNA. Translation: CCJ06346.1.
RefSeqiWP_014890380.1. NC_018485.1.
YP_006590997.1. NC_018485.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCJ06346; CCJ06346; BN69_0895.
GeneIDi13519097.
KEGGimsc:BN69_0895.

Phylogenomic databases

KOiK00031.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Methylocystis sp. strain SC2, an aerobic methanotroph with high-affinity methane oxidation potential."
    Dam B., Dam S., Kube M., Reinhardt R., Liesack W.
    J. Bacteriol. 194:6008-6009(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC2Imported.

Entry informationi

Entry nameiJ7QFI5_METSZ
AccessioniPrimary (citable) accession number: J7QFI5
Entry historyi
Integrated into UniProtKB/TrEMBL: October 31, 2012
Last sequence update: October 31, 2012
Last modified: February 4, 2015
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.