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J7LHN1 (J7LHN1_LACHE) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 11. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AFR21191.1
ORF Names:R0052_00795 EMBL AFR21191.1
OrganismLactobacillus helveticus R0052 [Complete proteome] EMBL AFR21191.1
Taxonomic identifier880633 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain134 – 343210ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2971Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3101Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3101Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3121Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
J7LHN1 [UniParc].

Last modified October 31, 2012. Version 1.
Checksum: 36F8C5F577C933F1

FASTA36040,318
        10         20         30         40         50         60 
MTKKTQVGLI FGGNSSEYEV SIVSGRNIYK AIDKEKFDVH PIWITNEGYF ANEEESFKVL 

        70         80         90        100        110        120 
EDPSYQVKNP HKVHNISNLI ELENLPEIDV FFPIVHGNLG EDGVLQGLFR LMNKPFVGDD 

       130        140        150        160        170        180 
ILAAAVTMDK EFTKILAQRV GVPVADWIAI KRFEYDDKNN DKLDYEKVAE KLGRDMFVKP 

       190        200        210        220        230        240 
SNQGSSVGVS HVTNADEYAA ALKEAFKYDD KVLVEKTVPG TEVETAVLGN DKPIVAGVGQ 

       250        260        270        280        290        300 
ITNAKGSFYS YKNKYDDNST SKLQIPADLP QEIIDTVRRN ARKVYEVTEC SGMARIDSML 

       310        320        330        340        350        360 
TPGGKVVLTE VNALPGFTNI SMYPKLFEEA GVPYTELITR LIEVGMERFD HKKTLLHKHD 

« Hide

References

[1]"Complete Genome Sequence of Lactobacillus helveticus R0052, a Commercial Probiotic Strain."
Tompkins T.A., Barreau G., Broadbent J.R.
J. Bacteriol. 194:6349-6349(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: R0052 EMBL AFR21191.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003799 Genomic DNA. Translation: AFR21191.1.
RefSeqYP_006654995.1. NC_018528.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFR21191; AFR21191; R0052_00795.
GeneID13555109.
KEGGlhr:R0052_00795.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameJ7LHN1_LACHE
AccessionPrimary (citable) accession number: J7LHN1
Entry history
Integrated into UniProtKB/TrEMBL: October 31, 2012
Last sequence update: October 31, 2012
Last modified: June 11, 2014
This is version 11 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)