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J7LHN1

- J7LHN1_LACHE

UniProt

J7LHN1 - J7LHN1_LACHE

Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Lactobacillus helveticus R0052
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 13 (01 Oct 2014)
      Sequence version 1 (31 Oct 2012)
      Previous versions | rss
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    Functioni

    Cell wall formation.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi297 – 2971Magnesium or manganese 1UniRule annotation
    Metal bindingi310 – 3101Magnesium or manganese 1UniRule annotation
    Metal bindingi310 – 3101Magnesium or manganese 2UniRule annotation
    Metal bindingi312 – 3121Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi169 – 22456ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. D-alanine-D-alanine ligase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    2. regulation of cell shape Source: UniProtKB-KW

    Keywords - Molecular functioni

    LigaseUniRule annotationSAAS annotationImported

    Keywords - Biological processi

    Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00219.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
    Alternative name(s):
    D-Ala-D-Ala ligaseUniRule annotation
    D-alanylalanine synthetaseUniRule annotation
    Gene namesi
    Name:ddlUniRule annotationImported
    ORF Names:R0052_00795Imported
    OrganismiLactobacillus helveticus R0052Imported
    Taxonomic identifieri880633 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000003775: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini134 – 343210ATP-graspUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the D-alanine--D-alanine ligase family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation
    Contains ATP-grasp domain.SAAS annotation

    Phylogenomic databases

    KOiK01921.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPiMF_00047. Dala_Dala_lig.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR23132. PTHR23132. 1 hit.
    PfamiPF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    J7LHN1-1 [UniParc]FASTAAdd to Basket

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    MTKKTQVGLI FGGNSSEYEV SIVSGRNIYK AIDKEKFDVH PIWITNEGYF    50
    ANEEESFKVL EDPSYQVKNP HKVHNISNLI ELENLPEIDV FFPIVHGNLG 100
    EDGVLQGLFR LMNKPFVGDD ILAAAVTMDK EFTKILAQRV GVPVADWIAI 150
    KRFEYDDKNN DKLDYEKVAE KLGRDMFVKP SNQGSSVGVS HVTNADEYAA 200
    ALKEAFKYDD KVLVEKTVPG TEVETAVLGN DKPIVAGVGQ ITNAKGSFYS 250
    YKNKYDDNST SKLQIPADLP QEIIDTVRRN ARKVYEVTEC SGMARIDSML 300
    TPGGKVVLTE VNALPGFTNI SMYPKLFEEA GVPYTELITR LIEVGMERFD 350
    HKKTLLHKHD 360
    Length:360
    Mass (Da):40,318
    Last modified:October 31, 2012 - v1
    Checksum:i36F8C5F577C933F1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003799 Genomic DNA. Translation: AFR21191.1.
    RefSeqiWP_014918107.1. NC_018528.1.
    YP_006654995.1. NC_018528.1.

    Genome annotation databases

    EnsemblBacteriaiAFR21191; AFR21191; R0052_00795.
    GeneIDi13555109.
    KEGGilhr:R0052_00795.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003799 Genomic DNA. Translation: AFR21191.1 .
    RefSeqi WP_014918107.1. NC_018528.1.
    YP_006654995.1. NC_018528.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AFR21191 ; AFR21191 ; R0052_00795 .
    GeneIDi 13555109.
    KEGGi lhr:R0052_00795.

    Phylogenomic databases

    KOi K01921.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPi MF_00047. Dala_Dala_lig.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR23132. PTHR23132. 1 hit.
    Pfami PF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete Genome Sequence of Lactobacillus helveticus R0052, a Commercial Probiotic Strain."
      Tompkins T.A., Barreau G., Broadbent J.R.
      J. Bacteriol. 194:6349-6349(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: R0052Imported.

    Entry informationi

    Entry nameiJ7LHN1_LACHE
    AccessioniPrimary (citable) accession number: J7LHN1
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 31, 2012
    Last sequence update: October 31, 2012
    Last modified: October 1, 2014
    This is version 13 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3