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J7LH11 (TPS8_PHYDL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 10. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
(+)-epi-alpha-bisabolol synthase

EC=4.2.3.138
Alternative name(s):
Terpene synthase 8
Short name=LdTPS8
OrganismPhyla dulcis (Aztec sweet herb) (Lippia dulcis)
Taxonomic identifier542674 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesVerbenaceaeLantaneaePhyla

Protein attributes

Sequence length546 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sesquiterpene synthase involved in the biosynthesis of (+)-epi-alpha-bisabolol, a precursor of the natural sweetner hernandulcin. Ref.1

Catalytic activity

(2E,6E)-farnesyl diphosphate + H2O = (+)-epi-alpha-bisabolol + diphosphate. Ref.1

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+ By similarity.

Sequence similarities

Belongs to the terpene synthase family.

Biophysicochemical properties

Kinetic parameters:

Kcat is 0.04 sec (-1) for (2E,6E)-farnesyl diphosphate.

KM=4.8 µM for (2E,6E)-farnesyl diphosphate Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 546546(+)-epi-alpha-bisabolol synthase
PRO_0000421955

Regions

Motif297 – 3015DDXXD motif

Sites

Metal binding2971Magnesium 1 By similarity
Metal binding2971Magnesium 2 By similarity
Metal binding3011Magnesium 1 By similarity
Metal binding3011Magnesium 2 By similarity
Metal binding4411Magnesium 3 By similarity
Metal binding4451Magnesium 3 By similarity
Metal binding4491Magnesium 3 By similarity

Sequences

Sequence LengthMass (Da)Tools
J7LH11 [UniParc].

Last modified October 31, 2012. Version 1.
Checksum: DE64E5D3475494B5

FASTA54663,782
        10         20         30         40         50         60 
MNSTSRRSAN YKPTIWNNEY LQSLNSIYGE KRFLEQAEKL KDEVRMLLEK TSDPLDHIEL 

        70         80         90        100        110        120 
VDVLQRLAIS YHFTEYIDRN LKNIYDILID GRRWNHADNL HATTLSFRLL RQHGYQVSPE 

       130        140        150        160        170        180 
VFRNFMDETG NFKKNLCDDI KGLLSLYEAS YLLTEGETIM DSAQAFATHH LKQKLEENMN 

       190        200        210        220        230        240 
KNLGDEIAHA LELPLHWRVP KLDVRWSIDA YERRQDMNPL LLELAKLDFN IAQSMYQDEL 

       250        260        270        280        290        300 
KELSRWYSKT HLPEKLAFAR DRLVESYLWG LGLASEPHHK YCRMMVAQST TLISIIDDIY 

       310        320        330        340        350        360 
DVYGTLDELQ LFTHAVDRWD IKYLEQLPEY MQICFLALFN TVNERSYDFL LDKGFNVIPH 

       370        380        390        400        410        420 
SSYRWAELCK TYLIEANWYH SGYKPSLNEY LNQGLISVAG PHALSHTYLC MTDSLKEKHI 

       430        440        450        460        470        480 
LDLRTNPPVI KWVSILVRLA DDLGTSTDEL KRGDNPKSIQ CHMHDTGCNE EETRAYIKNL 

       490        500        510        520        530        540 
IGSTWKKINK DVLMNFEYSM DFRTAAMNGA RVSQFMYQYD DDGHGVPEGK SKERVCSLIV 


EPIPLP 

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References

[1]"Molecular cloning and characterization of (+)-epi-alpha-bisabolol synthase, catalyzing the first step in the biosynthesis of the natural sweetener, hernandulcin, in Lippia dulcis."
Attia M., Kim S.U., Ro D.K.
Arch. Biochem. Biophys. 527:37-44(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Web resources

Protein Spotlight

The taste of sweet - Issue 148 of May 2013

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
JQ731636 mRNA. Translation: AFR23372.1.

3D structure databases

ProteinModelPortalJ7LH11.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00213.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTPS8_PHYDL
AccessionPrimary (citable) accession number: J7LH11
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 31, 2012
Last modified: February 19, 2014
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PATHWAY comments

Index of metabolic and biosynthesis pathways