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J7LH11

- TPS8_PHYDL

UniProt

J7LH11 - TPS8_PHYDL

Protein

(+)-epi-alpha-bisabolol synthase

Gene
N/A
Organism
Phyla dulcis (Aztec sweet herb) (Lippia dulcis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 11 (01 Oct 2014)
      Sequence version 1 (31 Oct 2012)
      Previous versions | rss
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    Functioni

    Sesquiterpene synthase involved in the biosynthesis of (+)-epi-alpha-bisabolol, a precursor of the natural sweetner hernandulcin.1 Publication

    Catalytic activityi

    (2E,6E)-farnesyl diphosphate + H2O = (+)-epi-alpha-bisabolol + diphosphate.1 Publication

    Cofactori

    Binds 3 magnesium ions per subunit.By similarity

    Kineticsi

    Kcat is 0.04 sec (-1) for (2E,6E)-farnesyl diphosphate.

    1. KM=4.8 µM for (2E,6E)-farnesyl diphosphate1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi297 – 2971Magnesium 1By similarity
    Metal bindingi297 – 2971Magnesium 2By similarity
    Metal bindingi301 – 3011Magnesium 1By similarity
    Metal bindingi301 – 3011Magnesium 2By similarity
    Metal bindingi441 – 4411Magnesium 3By similarity
    Metal bindingi445 – 4451Magnesium 3By similarity
    Metal bindingi449 – 4491Magnesium 3By similarity

    GO - Molecular functioni

    1. magnesium ion binding Source: InterPro
    2. sesquiterpene synthase activity Source: UniProtKB

    GO - Biological processi

    1. (+)-epi-alpha-bisabolol biosynthetic process Source: UniProtKB
    2. farnesyl diphosphate catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00213.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    (+)-epi-alpha-bisabolol synthase (EC:4.2.3.138)
    Alternative name(s):
    Terpene synthase 8
    Short name:
    LdTPS8
    OrganismiPhyla dulcis (Aztec sweet herb) (Lippia dulcis)
    Taxonomic identifieri542674 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesVerbenaceaeLantaneaePhyla

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 546546(+)-epi-alpha-bisabolol synthasePRO_0000421955Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliJ7LH11.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi297 – 3015DDXXD motif

    Domaini

    The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.By similarity

    Sequence similaritiesi

    Belongs to the terpene synthase family.Curated

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    1.50.30.10. 1 hit.
    InterProiIPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 1 hit.
    SSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    J7LH11-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSTSRRSAN YKPTIWNNEY LQSLNSIYGE KRFLEQAEKL KDEVRMLLEK    50
    TSDPLDHIEL VDVLQRLAIS YHFTEYIDRN LKNIYDILID GRRWNHADNL 100
    HATTLSFRLL RQHGYQVSPE VFRNFMDETG NFKKNLCDDI KGLLSLYEAS 150
    YLLTEGETIM DSAQAFATHH LKQKLEENMN KNLGDEIAHA LELPLHWRVP 200
    KLDVRWSIDA YERRQDMNPL LLELAKLDFN IAQSMYQDEL KELSRWYSKT 250
    HLPEKLAFAR DRLVESYLWG LGLASEPHHK YCRMMVAQST TLISIIDDIY 300
    DVYGTLDELQ LFTHAVDRWD IKYLEQLPEY MQICFLALFN TVNERSYDFL 350
    LDKGFNVIPH SSYRWAELCK TYLIEANWYH SGYKPSLNEY LNQGLISVAG 400
    PHALSHTYLC MTDSLKEKHI LDLRTNPPVI KWVSILVRLA DDLGTSTDEL 450
    KRGDNPKSIQ CHMHDTGCNE EETRAYIKNL IGSTWKKINK DVLMNFEYSM 500
    DFRTAAMNGA RVSQFMYQYD DDGHGVPEGK SKERVCSLIV EPIPLP 546
    Length:546
    Mass (Da):63,782
    Last modified:October 31, 2012 - v1
    Checksum:iDE64E5D3475494B5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JQ731636 mRNA. Translation: AFR23372.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The taste of sweet - Issue 148 of May 2013

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JQ731636 mRNA. Translation: AFR23372.1 .

    3D structure databases

    ProteinModelPortali J7LH11.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00213 .

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    1.50.30.10. 1 hit.
    InterProi IPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48239. SSF48239. 1 hit.
    SSF48576. SSF48576. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of (+)-epi-alpha-bisabolol synthase, catalyzing the first step in the biosynthesis of the natural sweetener, hernandulcin, in Lippia dulcis."
      Attia M., Kim S.U., Ro D.K.
      Arch. Biochem. Biophys. 527:37-44(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiTPS8_PHYDL
    AccessioniPrimary (citable) accession number: J7LH11
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2013
    Last sequence update: October 31, 2012
    Last modified: October 1, 2014
    This is version 11 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3