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J7LH11

- TPS8_PHYDL

UniProt

J7LH11 - TPS8_PHYDL

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Protein
(+)-epi-alpha-bisabolol synthase
Gene
N/A
Organism
Phyla dulcis (Aztec sweet herb) (Lippia dulcis)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Sesquiterpene synthase involved in the biosynthesis of (+)-epi-alpha-bisabolol, a precursor of the natural sweetner hernandulcin.1 Publication

Catalytic activityi

(2E,6E)-farnesyl diphosphate + H2O = (+)-epi-alpha-bisabolol + diphosphate.1 Publication

Cofactori

Binds 3 magnesium ions per subunit By similarity.

Kineticsi

Kcat is 0.04 sec (-1) for (2E,6E)-farnesyl diphosphate.

  1. KM=4.8 µM for (2E,6E)-farnesyl diphosphate1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi297 – 2971Magnesium 1 By similarity
Metal bindingi297 – 2971Magnesium 2 By similarity
Metal bindingi301 – 3011Magnesium 1 By similarity
Metal bindingi301 – 3011Magnesium 2 By similarity
Metal bindingi441 – 4411Magnesium 3 By similarity
Metal bindingi445 – 4451Magnesium 3 By similarity
Metal bindingi449 – 4491Magnesium 3 By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. sesquiterpene synthase activity Source: UniProtKB

GO - Biological processi

  1. (+)-epi-alpha-bisabolol biosynthetic process Source: UniProtKB
  2. farnesyl diphosphate catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00213.

Names & Taxonomyi

Protein namesi
Recommended name:
(+)-epi-alpha-bisabolol synthase (EC:4.2.3.138)
Alternative name(s):
Terpene synthase 8
Short name:
LdTPS8
OrganismiPhyla dulcis (Aztec sweet herb) (Lippia dulcis)
Taxonomic identifieri542674 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesVerbenaceaeLantaneaePhyla

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 546546(+)-epi-alpha-bisabolol synthase
PRO_0000421955Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliJ7LH11.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi297 – 3015DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+ By similarity.

Sequence similaritiesi

Belongs to the terpene synthase family.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

J7LH11-1 [UniParc]FASTAAdd to Basket

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MNSTSRRSAN YKPTIWNNEY LQSLNSIYGE KRFLEQAEKL KDEVRMLLEK    50
TSDPLDHIEL VDVLQRLAIS YHFTEYIDRN LKNIYDILID GRRWNHADNL 100
HATTLSFRLL RQHGYQVSPE VFRNFMDETG NFKKNLCDDI KGLLSLYEAS 150
YLLTEGETIM DSAQAFATHH LKQKLEENMN KNLGDEIAHA LELPLHWRVP 200
KLDVRWSIDA YERRQDMNPL LLELAKLDFN IAQSMYQDEL KELSRWYSKT 250
HLPEKLAFAR DRLVESYLWG LGLASEPHHK YCRMMVAQST TLISIIDDIY 300
DVYGTLDELQ LFTHAVDRWD IKYLEQLPEY MQICFLALFN TVNERSYDFL 350
LDKGFNVIPH SSYRWAELCK TYLIEANWYH SGYKPSLNEY LNQGLISVAG 400
PHALSHTYLC MTDSLKEKHI LDLRTNPPVI KWVSILVRLA DDLGTSTDEL 450
KRGDNPKSIQ CHMHDTGCNE EETRAYIKNL IGSTWKKINK DVLMNFEYSM 500
DFRTAAMNGA RVSQFMYQYD DDGHGVPEGK SKERVCSLIV EPIPLP 546
Length:546
Mass (Da):63,782
Last modified:October 31, 2012 - v1
Checksum:iDE64E5D3475494B5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JQ731636 mRNA. Translation: AFR23372.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

The taste of sweet - Issue 148 of May 2013

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JQ731636 mRNA. Translation: AFR23372.1 .

3D structure databases

ProteinModelPortali J7LH11.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00213 .

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProi IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of (+)-epi-alpha-bisabolol synthase, catalyzing the first step in the biosynthesis of the natural sweetener, hernandulcin, in Lippia dulcis."
    Attia M., Kim S.U., Ro D.K.
    Arch. Biochem. Biophys. 527:37-44(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiTPS8_PHYDL
AccessioniPrimary (citable) accession number: J7LH11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 31, 2012
Last modified: February 19, 2014
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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