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Protein

(+)-epi-alpha-bisabolol synthase

Gene
N/A
Organism
Phyla dulcis (Aztec sweet herb) (Lippia dulcis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sesquiterpene synthase involved in the biosynthesis of (+)-epi-alpha-bisabolol, a precursor of the natural sweetner hernandulcin.1 Publication

Catalytic activityi

(2E,6E)-farnesyl diphosphate + H2O = (+)-epi-alpha-bisabolol + diphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Kineticsi

Kcat is 0.04 sec (-1) for (2E,6E)-farnesyl diphosphate.

  1. KM=4.8 µM for (2E,6E)-farnesyl diphosphate1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi297 – 2971Magnesium 1By similarity
Metal bindingi297 – 2971Magnesium 2By similarity
Metal bindingi301 – 3011Magnesium 1By similarity
Metal bindingi301 – 3011Magnesium 2By similarity
Metal bindingi441 – 4411Magnesium 3By similarity
Metal bindingi445 – 4451Magnesium 3By similarity
Metal bindingi449 – 4491Magnesium 3By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. sesquiterpene synthase activity Source: UniProtKB

GO - Biological processi

  1. (+)-epi-alpha-bisabolol biosynthetic process Source: UniProtKB
  2. farnesyl diphosphate catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00213.

Names & Taxonomyi

Protein namesi
Recommended name:
(+)-epi-alpha-bisabolol synthase (EC:4.2.3.138)
Alternative name(s):
Terpene synthase 8
Short name:
LdTPS8
OrganismiPhyla dulcis (Aztec sweet herb) (Lippia dulcis)
Taxonomic identifieri542674 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesVerbenaceaeLantaneaePhyla

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 546546(+)-epi-alpha-bisabolol synthasePRO_0000421955Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliJ7LH11.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi297 – 3015DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.By similarity

Sequence similaritiesi

Belongs to the terpene synthase family.Curated

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

J7LH11-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSTSRRSAN YKPTIWNNEY LQSLNSIYGE KRFLEQAEKL KDEVRMLLEK
60 70 80 90 100
TSDPLDHIEL VDVLQRLAIS YHFTEYIDRN LKNIYDILID GRRWNHADNL
110 120 130 140 150
HATTLSFRLL RQHGYQVSPE VFRNFMDETG NFKKNLCDDI KGLLSLYEAS
160 170 180 190 200
YLLTEGETIM DSAQAFATHH LKQKLEENMN KNLGDEIAHA LELPLHWRVP
210 220 230 240 250
KLDVRWSIDA YERRQDMNPL LLELAKLDFN IAQSMYQDEL KELSRWYSKT
260 270 280 290 300
HLPEKLAFAR DRLVESYLWG LGLASEPHHK YCRMMVAQST TLISIIDDIY
310 320 330 340 350
DVYGTLDELQ LFTHAVDRWD IKYLEQLPEY MQICFLALFN TVNERSYDFL
360 370 380 390 400
LDKGFNVIPH SSYRWAELCK TYLIEANWYH SGYKPSLNEY LNQGLISVAG
410 420 430 440 450
PHALSHTYLC MTDSLKEKHI LDLRTNPPVI KWVSILVRLA DDLGTSTDEL
460 470 480 490 500
KRGDNPKSIQ CHMHDTGCNE EETRAYIKNL IGSTWKKINK DVLMNFEYSM
510 520 530 540
DFRTAAMNGA RVSQFMYQYD DDGHGVPEGK SKERVCSLIV EPIPLP
Length:546
Mass (Da):63,782
Last modified:October 31, 2012 - v1
Checksum:iDE64E5D3475494B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JQ731636 mRNA. Translation: AFR23372.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

The taste of sweet - Issue 148 of May 2013

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JQ731636 mRNA. Translation: AFR23372.1.

3D structure databases

ProteinModelPortaliJ7LH11.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00213.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of (+)-epi-alpha-bisabolol synthase, catalyzing the first step in the biosynthesis of the natural sweetener, hernandulcin, in Lippia dulcis."
    Attia M., Kim S.U., Ro D.K.
    Arch. Biochem. Biophys. 527:37-44(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiTPS8_PHYDL
AccessioniPrimary (citable) accession number: J7LH11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 31, 2012
Last modified: April 1, 2015
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.