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J7GCH4 (J7GCH4_ENTCL) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 7. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AFP68858.1
ORF Names:ECENHK_04855 EMBL AFP68858.1
OrganismEnterobacter cloacae subsp. cloacae ENHKU01 EMBL AFP68858.1
Taxonomic identifier1211025 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter › Enterobacter cloacae complex ›

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS000291

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS000291

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS000291

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS000291

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS000291.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047 SAAS SAAS000291

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain145 – 348204ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding175 – 23056ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3021Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3171Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
J7GCH4 [UniParc].

Last modified October 31, 2012. Version 1.
Checksum: E5282929E755603B

FASTA36639,233
        10         20         30         40         50         60 
MAKQRVGIVF GGKSAEHEVS LQSAKNIVDA IDKSRFDVVL LGIDKQGQWH VNDASQYLLN 

        70         80         90        100        110        120 
AHDPAHIALN PSDISVATVP GVMKGQLIDA GNAQALTQID VVFPIVHGTL GEDGSLQGML 

       130        140        150        160        170        180 
RMANLPFVGS DVLGSAACMD KDVTKRLLRD AGLNIAPFVT LTRANRDKYS FAQVSGQLGL 

       190        200        210        220        230        240 
PLFVKPANQG SSVGVSKVAS EAEFTQAVRL AFEFDHKVVI EQGIKGREIE CAVLGNDFPQ 

       250        260        270        280        290        300 
ASTCGEVVLN SDFYSYDTKY IDDKGAQVVV PADIDPAIND KIRAIAISAY QTLGCSGMAR 

       310        320        330        340        350        360 
VDVFLTPEND VVINEINTLP GFTNISMYPK LWQASGLGYS ELITRLIELA LERHAADSAL 


KSSVNG

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References

[1]"Complete Genome Sequence of the Endophytic Enterobacter cloacae subsp. cloacae Strain ENHKU01."
Liu W.Y., Chung K.M., Wong C.F., Jiang J.W., Hui R.K., Leung F.C.
J. Bacteriol. 194:5965-5965(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ENHKU01 EMBL AFP68858.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003737 Genomic DNA. Translation: AFP68858.1.
RefSeqYP_006577482.1. NC_018405.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFP68858; AFP68858; ECENHK_04855.
GeneID13459813.
KEGGeno:ECENHK_04855.

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameJ7GCH4_ENTCL
AccessionPrimary (citable) accession number: J7GCH4
Entry history
Integrated into UniProtKB/TrEMBL: October 31, 2012
Last sequence update: October 31, 2012
Last modified: May 29, 2013
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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