ID   J3KN16_HUMAN            Unreviewed;      2017 AA.
AC   J3KN16;
DT   03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT   03-OCT-2012, sequence version 1.
DT   27-MAR-2024, entry version 91.
DE   SubName: Full=Ecm29 proteasome adaptor and scaffold {ECO:0000313|Ensembl:ENSP00000259335.4};
GN   Name=ECPAS {ECO:0000313|Ensembl:ENSP00000259335.4};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|Ensembl:ENSP00000259335.4, ECO:0000313|Proteomes:UP000005640};
RN   [1] {ECO:0000313|Ensembl:ENSP00000259335.4, ECO:0000313|Proteomes:UP000005640}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15164053; DOI=10.1038/nature02465;
RA   Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA   Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA   Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA   Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA   Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA   Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA   Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA   Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA   Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA   Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA   Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA   Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA   Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA   Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA   Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA   Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA   Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA   Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA   McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA   Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA   Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA   Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA   Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA   West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA   Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.,
RA   Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA   Dunham I.;
RT   "DNA sequence and analysis of human chromosome 9.";
RL   Nature 429:369-374(2004).
RN   [2] {ECO:0007829|PubMed:20068231}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA   Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA   Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT   "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT   site occupancy during mitosis.";
RL   Sci. Signal. 3:RA3-RA3(2010).
RN   [3] {ECO:0007829|PubMed:21269460}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [4] {ECO:0007829|PubMed:21406692}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA   Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA   Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT   "System-wide temporal characterization of the proteome and phosphoproteome
RT   of human embryonic stem cell differentiation.";
RL   Sci. Signal. 4:RS3-RS3(2011).
RN   [5] {ECO:0007829|PubMed:23186163}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=23186163;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
RN   [6] {ECO:0007829|PubMed:25114211}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25114211;
RA   Impens F., Radoshevich L., Cossart P., Ribet D.;
RT   "Mapping of SUMO sites and analysis of SUMOylation changes induced by
RT   external stimuli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 111:12432-12437(2014).
RN   [7] {ECO:0000313|Ensembl:ENSP00000259335.4}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
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DR   EMBL; AL159168; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AL354661; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_001073867.1; NM_001080398.1.
DR   IntAct; J3KN16; 4.
DR   MINT; J3KN16; -.
DR   iPTMnet; J3KN16; -.
DR   MassIVE; J3KN16; -.
DR   MaxQB; J3KN16; -.
DR   PeptideAtlas; J3KN16; -.
DR   Antibodypedia; 29523; 56 antibodies from 15 providers.
DR   Ensembl; ENST00000259335.8; ENSP00000259335.4; ENSG00000136813.15.
DR   UCSC; uc004bfe.1; human.
DR   HGNC; HGNC:29020; ECPAS.
DR   VEuPathDB; HostDB:ENSG00000136813; -.
DR   GeneTree; ENSGT00940000153612; -.
DR   PhylomeDB; J3KN16; -.
DR   TreeFam; TF314213; -.
DR   BioGRID-ORCS; 23392; 12 hits in 1157 CRISPR screens.
DR   GenomeRNAi; 23392; -.
DR   Proteomes; UP000005640; Chromosome 9.
DR   Bgee; ENSG00000136813; Expressed in gastrocnemius and 212 other cell types or tissues.
DR   ExpressionAtlas; J3KN16; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR   GO; GO:0060090; F:molecular adaptor activity; IEA:InterPro.
DR   GO; GO:0043248; P:proteasome assembly; IEA:InterPro.
DR   Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 4.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR024372; Ecm29.
DR   PANTHER; PTHR23346:SF19; PROTEASOME ADAPTER AND SCAFFOLD PROTEIN ECM29; 1.
DR   PANTHER; PTHR23346; TRANSLATIONAL ACTIVATOR GCN1-RELATED; 1.
DR   Pfam; PF13001; Ecm29; 1.
DR   Pfam; PF12755; Vac14_Fab1_bd; 1.
DR   SUPFAM; SSF48371; ARM repeat; 3.
PE   1: Evidence at protein level;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Proteomics identification {ECO:0007829|EPD:J3KN16,
KW   ECO:0007829|MaxQB:J3KN16};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005640};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT   REGION          48..181
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          365..389
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        57..72
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        365..385
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   2017 AA;  223694 MW;  0194A2BDED3768EC CRC64;
     MTSRTKRRQK VKISISKINH SSYRKEHRPF WEHQPPPHQN WAVGGRCGAG ARPGLSQRRG
     REVRREAQGG ASSRRLRARL YFSESTSLPL DRRGSPGPLG PAPHGLRRAA PRGPARRTPR
     STALTSRRRA QRPRHGDRLR LRSEARPRAA GDWPCARGGA VRPGRPHRPP RVESARGAHA
     GRAATDQLER VFLRLGHAET DEQLQNIISK FLPPVLLKLS STQEGVRKKV MELLVHLNKR
     IKSRPKIQLP VETLLVQYQD PAAVSFVTNF TIIYVKMGYP RLPVEKQCEL APTLLTAMEG
     KPQPQQDSLM HLLIPTLFHM KYPVESSKSA SPFNLAEKPK TVQLLLDFML DVLLMPYGYV
     LNESQSRQNS SSAQGSSSNS GGGSGIPQPP PGMSFYAAKR VIGDNPWTPE QLEQCKLGIV
     KFIEAEQVPE LEAVLHLVIA SSDTRHSVAT AADLELKSKQ SLIDWNNPAI INKMYKVYLG
     DIPLKTKEGA VLKPELKRDP VSTRVKLKIV PHLLRSRQAA ETFPANIQVV YDGLFGTNTN
     SKLRTLSLQF VHHICITCPE IKIKPLGPML LNGLTKLINE YKEDPKLLSM AYSAVGKLSS
     RMPHLFTKDI ALVQQLFEAL CKEEPETRLA IQEALSMMVG AYSTLEGAQR TLMEALVASY
     LIKPEVQVRQ VAVKFASTVF PSDHIPSRYL LLLAAGDPRE EVHGEAQRVL RCLPGRNRKE
     STSEQMPSFP EMVYYIQEKA SHRMKTPVKY MTGTTVLPFN PAAFGEIVLY LRMCLAHSAG
     VVPTSQSLAD MQDHAPAIGR YIRTLMSSGQ MAPSSSNKSG ETNPVQIYIG LLQQLLAGVG
     GLPVMYCLLE AVSVYPEKLA TKFVDKTEWI KSLMNNSKEE MRELAALFYS VVVSTVSGNE
     LKSMIEQLIK TTKDNHSPEI QHGSLLALGF TVGRYLAKKK MRMSEQQDLE RNADTLPDQE
     ELIQSATETI GSFLDSTSPL LAIAACTALG EIGRNGPLPI PSEGSGFTKL HLVESLLSRI
     PSSKETNKMK ERAIQTLGYF PVGDGDFPHQ KLLLQGLMDS VEAKQIELQF TIGEAITSAA
     IGTSSVAARD AWQMTEEEYT PPAGAKVNDV VPWVLDVILN KHIISPNPHV RQAACIWLLS
     LVRKLSTHKE VKSHLKEIQS AFVSVLSEND ELSQDVASKG LGLVYELGNE QDQQELVSTL
     VETLMTGKRV KHEVSGETVV FQGGALGKTP DGQGLSTYKE LCSLASDLSQ PDLVYKFMNL
     ANHHAMWNSR KGAAFGFNVI ATRAGEQLAP FLPQLVPRLY RYQFDPNLGI RQAMTSIWNA
     LVTDKSMVDK YLKEILQDLV KNLTSNMWRV RESSCLALND LLRGRPLDDI IDKLPEIWET
     LFRVQDDIKE SVRKAAELAL KTLSKVCVKM CDPAKGAAGQ RTIAALLPCL LDKGMMSTVT
     EVRALSINTL VKISKSAGAM LKPHAPKLIP ALLESLSVLE PQVLNYLSLR ATEQEKAAMD
     SARLSAAKSS PMMETINMCL QYLDVSVLGE LVPRLCELIR SGVGLGTKGG CASVIVSLTT
     QCPQDLTPYS GKLMSALLSG LTDRNSVIQK SCAFAMGHLV RTSRDSSTEK LLQKLNGWYM
     EKEEPIYKTS CALTIHAIGR YSPDVLKNHA KEVLPLAFLG MHEIADEEKS EKEECNLWTE
     VWQENVPGSF GGIRLYLQEL ITITQKALQS QSWKMKAQGA IAMASIAKQT SSLVPPYLGM
     ILTALLQGLA GRTWAGKEEL LKAIACVVTA CSAELEKSVP NQPSTNEILQ AVLKECSKEN
     VKYKIVAISC AADILKATKE DRFQEFSNIV IPLIKKNSLE SSGVRTTKNE EENEKEKELQ
     LEYLLGAFES LGKAWPRNAE TQRCYRQELC KLMCERLKLS TWKVQLGVLQ SMNAFFQGLM
     LLEEEHADPE ALAEILLETC KSITYSLENK TYSSVRTEAL SVIELLLKKL EESKQWECLT
     SECRVLLIES LATMEPDSRP ELQEKAALLK KTLENLE
//