UniProtKB - I7LNH2 (I7LNH2_METBM)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
Ribulose bisphosphate carboxylase
Gene
rbcL
Organism
Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)
Status
Functioni
Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation
Miscellaneous
Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation
Catalytic activityi
2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation
Cofactori
Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 152 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 154 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 178 | Magnesium; via carbamate groupUniRule annotation | 1 | |
Metal bindingi | 180 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 181 | MagnesiumUniRule annotation | 1 | |
Active sitei | 270 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 271 | SubstrateUniRule annotation | 1 | |
Binding sitei | 303 | SubstrateUniRule annotation | 1 | |
Sitei | 310 | Transition state stabilizerUniRule annotation | 1 |
GO - Molecular functioni
- magnesium ion binding Source: UniProtKB-UniRule
- oxidoreductase activity Source: UniProtKB-KW
- ribulose-bisphosphate carboxylase activity Source: UniProtKB-UniRule
GO - Biological processi
- AMP catabolic process Source: UniProtKB-UniRule
- carbon fixation Source: UniProtKB-KW
Keywordsi
Molecular function | LyaseUniRule annotationImported, OxidoreductaseUniRule annotation |
Biological process | Carbon dioxide fixationUniRule annotation |
Ligand | MagnesiumUniRule annotation, Metal-bindingUniRule annotation |
Enzyme and pathway databases
BioCyci | MBOU1201294:G1HBB-2190-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)Short name: RuBisCOUniRule annotation |
Gene namesi | Name:rbcLUniRule annotationImported Ordered Locus Names:BN140_2166Imported |
Organismi | Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)Imported |
Taxonomic identifieri | 1201294 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus › |
Proteomesi |
|
PTM / Processingi
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 178 | N6-carboxylysineUniRule annotation | 1 |
Interactioni
Subunit structurei
Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 121 | RuBisCO_large_NInterPro annotationAdd BLAST | 114 | |
Domaini | 132 – 424 | RuBisCO_largeInterPro annotationAdd BLAST | 293 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 353 – 355 | Substrate bindingUniRule annotation | 3 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 188 – 208 | Sequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilSequence analysisPhylogenomic databases
KOi | K01601. |
OMAi | HRAMHAA. |
OrthoDBi | POG093Z02MJ. |
Family and domain databases
Gene3Di | 3.20.20.110. 1 hit. 3.30.70.150. 1 hit. |
HAMAPi | MF_01133. RuBisCO_L_type3. 1 hit. |
InterProi | View protein in InterPro IPR033966. RuBisCO. IPR017712. RuBisCO_III. IPR020878. RuBisCo_large_chain_AS. IPR000685. RuBisCO_lsu_C. IPR036376. RuBisCO_lsu_C_sf. IPR017443. RuBisCO_lsu_fd_N. IPR036422. RuBisCO_lsu_N_sf. |
Pfami | View protein in Pfam PF00016. RuBisCO_large. 1 hit. PF02788. RuBisCO_large_N. 1 hit. |
SFLDi | SFLDS00014. RuBisCO. 1 hit. |
SUPFAMi | SSF51649. SSF51649. 1 hit. SSF54966. SSF54966. 1 hit. |
TIGRFAMsi | TIGR03326. rubisco_III. 1 hit. |
PROSITEi | View protein in PROSITE PS00157. RUBISCO_LARGE. 1 hit. |
i Sequence
Sequence statusi: Complete.
I7LNH2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAIDWYEEFV DTDHTPGSDE LVALYYFEPA AGISREEAVG RIASESSTGT
60 70 80 90 100
WTTLFTMPPR MRDLQAKAFE IEGNYVKIAY PLALWEEGNA PQLLSGIAGN
110 120 130 140 150
VFGMKALESL RLIDASLPAE YLRHFKGPHF GMEGIRDMMK VHGRPLTGAV
160 170 180 190 200
PKPKVGFTAE EHAEVGYETW MGGFDFVKDD ENLTSLAFNR FEDRVRAMTK
210 220 230 240 250
MRDRAEQETG DVKSAFINIT ADTETMKKRA EMLADYGWNY AMIDVVVTGT
260 270 280 290 300
AAVATLRDHC SDLGLAIHAH RAMHAAFDRD EKHGITMQFL AKIMRLIGVA
310 320 330 340 350
QIHTGTAVGK LVGTKAEATV LADMVREKHT NAVDHMALDQ DWGAIKSAFP
360 370 380 390 400
VSSGGLHPGL VPDVLDIYGS DLVLLVSGGI HGHPKGTRAG AMATMQAIEA
410 420 430
WSEGETLEEK AKKAPELREA LEKWGYYKPK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HE964772 Genomic DNA. Translation: CCJ37089.1. |
RefSeqi | WP_014868062.1. NC_018227.2. |
Genome annotation databases
EnsemblBacteriai | CCJ37089; CCJ37089; BN140_2166. |
GeneIDi | 13354056. |
KEGGi | mbg:BN140_2166. |
PATRICi | fig|1201294.9.peg.2402. |
Similar proteinsi
Entry informationi
Entry namei | I7LNH2_METBM | |
Accessioni | I7LNH2Primary (citable) accession number: I7LNH2 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | October 3, 2012 |
Last sequence update: | October 3, 2012 | |
Last modified: | February 28, 2018 | |
This is version 36 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |
Miscellaneousi
Caution
Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation