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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei152 – 1521Proton acceptorUniRule annotation
Binding sitei154 – 1541SubstrateUniRule annotation
Metal bindingi178 – 1781Magnesium; via carbamate groupUniRule annotation
Metal bindingi180 – 1801MagnesiumUniRule annotation
Metal bindingi181 – 1811MagnesiumUniRule annotation
Active sitei270 – 2701Proton acceptorUniRule annotation
Binding sitei271 – 2711SubstrateUniRule annotation
Binding sitei303 – 3031SubstrateUniRule annotation
Sitei310 – 3101Transition state stabilizerUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciMBOU1201294:GLGD-2211-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotationImported
Ordered Locus Names:BN140_2166Imported
OrganismiMethanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)Imported
Taxonomic identifieri1201294 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
ProteomesiUP000009007 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei178 – 1781N6-carboxylysineUniRule annotation

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliI7LNH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni353 – 3553Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

KOiK01601.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3.
InterProiIPR017712. RuBisCO_III.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I7LNH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIDWYEEFV DTDHTPGSDE LVALYYFEPA AGISREEAVG RIASESSTGT
60 70 80 90 100
WTTLFTMPPR MRDLQAKAFE IEGNYVKIAY PLALWEEGNA PQLLSGIAGN
110 120 130 140 150
VFGMKALESL RLIDASLPAE YLRHFKGPHF GMEGIRDMMK VHGRPLTGAV
160 170 180 190 200
PKPKVGFTAE EHAEVGYETW MGGFDFVKDD ENLTSLAFNR FEDRVRAMTK
210 220 230 240 250
MRDRAEQETG DVKSAFINIT ADTETMKKRA EMLADYGWNY AMIDVVVTGT
260 270 280 290 300
AAVATLRDHC SDLGLAIHAH RAMHAAFDRD EKHGITMQFL AKIMRLIGVA
310 320 330 340 350
QIHTGTAVGK LVGTKAEATV LADMVREKHT NAVDHMALDQ DWGAIKSAFP
360 370 380 390 400
VSSGGLHPGL VPDVLDIYGS DLVLLVSGGI HGHPKGTRAG AMATMQAIEA
410 420 430
WSEGETLEEK AKKAPELREA LEKWGYYKPK
Length:430
Mass (Da):47,333
Last modified:October 3, 2012 - v1
Checksum:i045609878AD9C7AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE964772 Genomic DNA. Translation: CCJ37089.1.
RefSeqiWP_014868062.1. NC_018227.2.
YP_006545805.1. NC_018227.2.

Genome annotation databases

GeneIDi13354056.
KEGGimbg:BN140_2166.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE964772 Genomic DNA. Translation: CCJ37089.1.
RefSeqiWP_014868062.1. NC_018227.2.
YP_006545805.1. NC_018227.2.

3D structure databases

ProteinModelPortaliI7LNH2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13354056.
KEGGimbg:BN140_2166.

Phylogenomic databases

KOiK01601.

Enzyme and pathway databases

BioCyciMBOU1201294:GLGD-2211-MONOMER.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3.
InterProiIPR017712. RuBisCO_III.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the hydrogenotrophic, methanogenic archaeon Methanoculleus bourgensis strain MS2T, isolated from a sewage sludge digester."
    Maus I., Wibberg D., Stantscheff R., Eikmeyer F.G., Seffner A., Boelter J., Szczepanowski R., Blom J., Jaenicke S., Konig H., Puhler A., Schluter A.
    J. Bacteriol. 194:5487-5488(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43281 / DSM 3045 / OCM 15 / MS2Imported.

Entry informationi

Entry nameiI7LNH2_METBM
AccessioniPrimary (citable) accession number: I7LNH2
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: April 29, 2015
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.