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I7LNH2

- I7LNH2_METBM

UniProt

I7LNH2 - I7LNH2_METBM

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 14 (01 Oct 2014)
      Sequence version 1 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei152 – 1521Proton acceptorUniRule annotation
    Binding sitei154 – 1541SubstrateUniRule annotation
    Metal bindingi178 – 1781Magnesium; via carbamate groupUniRule annotation
    Metal bindingi180 – 1801MagnesiumUniRule annotation
    Metal bindingi181 – 1811MagnesiumUniRule annotation
    Active sitei270 – 2701Proton acceptorUniRule annotation
    Binding sitei271 – 2711SubstrateUniRule annotation
    Binding sitei303 – 3031SubstrateUniRule annotation
    Sitei310 – 3101Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: InterPro

    Keywords - Molecular functioni

    LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciMBOU1201294:GLGD-2211-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotationImported
    Ordered Locus Names:BN140_2166Imported
    OrganismiMethanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)Imported
    Taxonomic identifieri1201294 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
    ProteomesiUP000009007: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei178 – 1781N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliI7LNH2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni353 – 3553Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family.UniRule annotation
    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    KOiK01601.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProiIPR017712. RuBisCO_III.
    IPR020878. RuBisCo_large_chain_AS.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
    PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    I7LNH2-1 [UniParc]FASTAAdd to Basket

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    MAIDWYEEFV DTDHTPGSDE LVALYYFEPA AGISREEAVG RIASESSTGT    50
    WTTLFTMPPR MRDLQAKAFE IEGNYVKIAY PLALWEEGNA PQLLSGIAGN 100
    VFGMKALESL RLIDASLPAE YLRHFKGPHF GMEGIRDMMK VHGRPLTGAV 150
    PKPKVGFTAE EHAEVGYETW MGGFDFVKDD ENLTSLAFNR FEDRVRAMTK 200
    MRDRAEQETG DVKSAFINIT ADTETMKKRA EMLADYGWNY AMIDVVVTGT 250
    AAVATLRDHC SDLGLAIHAH RAMHAAFDRD EKHGITMQFL AKIMRLIGVA 300
    QIHTGTAVGK LVGTKAEATV LADMVREKHT NAVDHMALDQ DWGAIKSAFP 350
    VSSGGLHPGL VPDVLDIYGS DLVLLVSGGI HGHPKGTRAG AMATMQAIEA 400
    WSEGETLEEK AKKAPELREA LEKWGYYKPK 430
    Length:430
    Mass (Da):47,333
    Last modified:October 3, 2012 - v1
    Checksum:i045609878AD9C7AE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HE964772 Genomic DNA. Translation: CCJ37089.1.
    RefSeqiYP_006545805.1. NC_018227.2.

    Genome annotation databases

    EnsemblBacteriaiCCJ37089; CCJ37089; BN140_2166.
    GeneIDi13354056.
    KEGGimbg:BN140_2166.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HE964772 Genomic DNA. Translation: CCJ37089.1 .
    RefSeqi YP_006545805.1. NC_018227.2.

    3D structure databases

    ProteinModelPortali I7LNH2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CCJ37089 ; CCJ37089 ; BN140_2166 .
    GeneIDi 13354056.
    KEGGi mbg:BN140_2166.

    Phylogenomic databases

    KOi K01601.

    Enzyme and pathway databases

    BioCyci MBOU1201294:GLGD-2211-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProi IPR017712. RuBisCO_III.
    IPR020878. RuBisCo_large_chain_AS.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
    PROSITEi PS00157. RUBISCO_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the hydrogenotrophic, methanogenic archaeon Methanoculleus bourgensis strain MS2T, isolated from a sewage sludge digester."
      Maus I., Wibberg D., Stantscheff R., Eikmeyer F.G., Seffner A., Boelter J., Szczepanowski R., Blom J., Jaenicke S., Konig H., Puhler A., Schluter A.
      J. Bacteriol. 194:5487-5488(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43281 / DSM 3045 / OCM 15 / MS2Imported.

    Entry informationi

    Entry nameiI7LNH2_METBM
    AccessioniPrimary (citable) accession number: I7LNH2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 3, 2012
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 14 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3