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UniProtKB - I7LNH2 (I7LNH2_METBM)

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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei152Proton acceptorUniRule annotation1
Binding sitei154SubstrateUniRule annotation1
Metal bindingi178Magnesium; via carbamate groupUniRule annotation1
Metal bindingi180MagnesiumUniRule annotation1
Metal bindingi181MagnesiumUniRule annotation1
Active sitei270Proton acceptorUniRule annotation1
Binding sitei271SubstrateUniRule annotation1
Binding sitei303SubstrateUniRule annotation1
Sitei310Transition state stabilizerUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionLyaseUniRule annotationImported, OxidoreductaseUniRule annotation
Biological processCarbon dioxide fixationUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciMBOU1201294:GLGD-2167-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotationImported
Ordered Locus Names:BN140_2166Imported
OrganismiMethanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)Imported
Taxonomic identifieri1201294 [NCBI]
Taxonomic lineageiArchaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus ›
Proteomesi
  • UP000009007 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei178N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliI7LNH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 121RuBisCO_large_NInterPro annotationAdd BLAST114
Domaini132 – 424RuBisCO_largeInterPro annotationAdd BLAST293

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni353 – 355Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

KOiK01601.
OMAiHRAMHAA.
OrthoDBiPOG093Z02MJ.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiView protein in InterPro
IPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
PfamiView protein in Pfam
PF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
SFLDiSFLDS00014. RuBisCO. 1 hit.
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
PROSITEiView protein in PROSITE
PS00157. RUBISCO_LARGE. 1 hit.

Sequencei

Sequence statusi: Complete.

I7LNH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIDWYEEFV DTDHTPGSDE LVALYYFEPA AGISREEAVG RIASESSTGT 
        60         70         80         90        100
WTTLFTMPPR MRDLQAKAFE IEGNYVKIAY PLALWEEGNA PQLLSGIAGN 
       110        120        130        140        150
VFGMKALESL RLIDASLPAE YLRHFKGPHF GMEGIRDMMK VHGRPLTGAV 
       160        170        180        190        200
PKPKVGFTAE EHAEVGYETW MGGFDFVKDD ENLTSLAFNR FEDRVRAMTK 
       210        220        230        240        250
MRDRAEQETG DVKSAFINIT ADTETMKKRA EMLADYGWNY AMIDVVVTGT 
       260        270        280        290        300
AAVATLRDHC SDLGLAIHAH RAMHAAFDRD EKHGITMQFL AKIMRLIGVA 
       310        320        330        340        350
QIHTGTAVGK LVGTKAEATV LADMVREKHT NAVDHMALDQ DWGAIKSAFP 
       360        370        380        390        400
VSSGGLHPGL VPDVLDIYGS DLVLLVSGGI HGHPKGTRAG AMATMQAIEA 
       410        420        430
WSEGETLEEK AKKAPELREA LEKWGYYKPK
Length:430
Mass (Da):47,333
Last modified:October 3, 2012 - v1
Checksum:i045609878AD9C7AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE964772 Genomic DNA. Translation: CCJ37089.1.
RefSeqiWP_014868062.1. NC_018227.2.

Genome annotation databases

EnsemblBacteriaiCCJ37089; CCJ37089; BN140_2166.
GeneIDi13354056.
KEGGimbg:BN140_2166.
PATRICifig|1201294.9.peg.2402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE964772 Genomic DNA. Translation: CCJ37089.1.
RefSeqiWP_014868062.1. NC_018227.2.

3D structure databases

ProteinModelPortaliI7LNH2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCJ37089; CCJ37089; BN140_2166.
GeneIDi13354056.
KEGGimbg:BN140_2166.
PATRICifig|1201294.9.peg.2402.

Phylogenomic databases

KOiK01601.
OMAiHRAMHAA.
OrthoDBiPOG093Z02MJ.

Enzyme and pathway databases

BioCyciMBOU1201294:GLGD-2167-MONOMER.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiView protein in InterPro
IPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
PfamiView protein in Pfam
PF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
SFLDiSFLDS00014. RuBisCO. 1 hit.
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
PROSITEiView protein in PROSITE
PS00157. RUBISCO_LARGE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiI7LNH2_METBM
AccessioniPrimary (citable) accession number: I7LNH2
Entry historyiIntegrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: June 7, 2017
This is version 32 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.