Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

I7LLJ8 (I7LLJ8_METBM) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase HAMAP-Rule MF_00407

EC=6.5.1.1 HAMAP-Rule MF_00407
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:lig1 EMBL CCJ35449.1
Synonyms:lig HAMAP-Rule MF_00407
Ordered Locus Names:BN140_0526 EMBL CCJ35449.1
OrganismMethanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense) [Complete proteome] [HAMAP] EMBL CCJ35449.1
Taxonomic identifier1201294 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. RuleBase RU004196 HAMAP-Rule MF_00407

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2461N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2441ATP By similarity HAMAP-Rule MF_00407
Binding site2511ATP By similarity HAMAP-Rule MF_00407
Binding site2661ATP By similarity HAMAP-Rule MF_00407
Binding site2951ATP By similarity HAMAP-Rule MF_00407
Binding site3341ATP By similarity HAMAP-Rule MF_00407
Binding site4051ATP By similarity HAMAP-Rule MF_00407
Binding site4111ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
I7LLJ8 [UniParc].

Last modified October 3, 2012. Version 1.
Checksum: CEA0573FE66125EF

FASTA54861,152
        10         20         30         40         50         60 
MQFLEFARIC EHLEGISGRL DMIEQVSAVL PGLEDEELPI FVRFIMGRVF PDWSPKKLGV 

        70         80         90        100        110        120 
GPNLLYDAVA YVVGTKRSAV REAINATGDA GLAIEDLLAT KEQTSFFVQE MDLLEVYWDF 

       130        140        150        160        170        180 
ERVAAAEGAR SQREKLLVVR KLFANARPLE GRYLARLMLE ELRIGMGEGS VRDAVARAFD 

       190        200        210        220        230        240 
IDVRLVEHAH QAMNDLGEVA LLARRDPAAL SRVTIEPFRP VKMMLAQAGT IAGQIEDHGE 

       250        260        270        280        290        300 
VAVEYKYDGS RFQFHKVGNI CRIYSRRLEE VTGSLPDIVM HLGEATDHDV ILDGEVVAVQ 

       310        320        330        340        350        360 
DGRPMPFQYV IRRFRRKHQV NAMMEKIEVV PRVFDILYLD GETLMDRPLA ERRRILEEVL 

       370        380        390        400        410        420 
DAHIAPQFLV TDVAGAEAIY TEALNLGHEG VMVKVPSSPY TPGVRGRLWV KVKPGVETLD 

       430        440        450        460        470        480 
LAVIGAEWGE GRRAKMFGSF LLAVQDQGRL LPVGKVATGI TDEVLADLYA LLKDSVIARS 

       490        500        510        520        530        540 
GKEVTLEPKV VFEVGYSEIQ ASPNYASGYA LRFPRFVQVR EDKSVDEVET LDALAERYGE 


QKNGQGSL 

« Hide

References

[1]"Complete genome sequence of the hydrogenotrophic, methanogenic archaeon Methanoculleus bourgensis strain MS2T, isolated from a sewage sludge digester."
Maus I., Wibberg D., Stantscheff R., Eikmeyer F.G., Seffner A., Boelter J., Szczepanowski R., Blom J., Jaenicke S., Konig H., Puhler A., Schluter A.
J. Bacteriol. 194:5487-5488(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43281 / DSM 3045 / OCM 15 / MS2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
HE964772 Genomic DNA. Translation: CCJ35449.1.
RefSeqYP_006544165.1. NC_018227.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCCJ35449; CCJ35449; BN140_0526.
GeneID13354133.
KEGGmbg:BN140_0526.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK10747.

Enzyme and pathway databases

BioCycMBOU1201294:GLGD-538-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI7LLJ8_METBM
AccessionPrimary (citable) accession number: I7LLJ8
Entry history
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: June 11, 2014
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)