Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA ligase

Gene

lig1

Organism
Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei244 – 2441ATPUniRule annotation
Active sitei246 – 2461N6-AMP-lysine intermediateUniRule annotation
Binding sitei251 – 2511ATPUniRule annotation
Binding sitei266 – 2661ATPUniRule annotation
Binding sitei295 – 2951ATPUniRule annotation
Binding sitei334 – 3341ATPUniRule annotation
Binding sitei405 – 4051ATPUniRule annotation
Binding sitei411 – 4111ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBOU1201294:GLGD-538-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotationImported)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:lig1Imported
Synonyms:ligUniRule annotation
Ordered Locus Names:BN140_0526Imported
OrganismiMethanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)Imported
Taxonomic identifieri1201294 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
ProteomesiUP000009007 Componenti: Chromosome

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

KOiK10747.
OMAiDAEVDIC.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I7LLJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFLEFARIC EHLEGISGRL DMIEQVSAVL PGLEDEELPI FVRFIMGRVF
60 70 80 90 100
PDWSPKKLGV GPNLLYDAVA YVVGTKRSAV REAINATGDA GLAIEDLLAT
110 120 130 140 150
KEQTSFFVQE MDLLEVYWDF ERVAAAEGAR SQREKLLVVR KLFANARPLE
160 170 180 190 200
GRYLARLMLE ELRIGMGEGS VRDAVARAFD IDVRLVEHAH QAMNDLGEVA
210 220 230 240 250
LLARRDPAAL SRVTIEPFRP VKMMLAQAGT IAGQIEDHGE VAVEYKYDGS
260 270 280 290 300
RFQFHKVGNI CRIYSRRLEE VTGSLPDIVM HLGEATDHDV ILDGEVVAVQ
310 320 330 340 350
DGRPMPFQYV IRRFRRKHQV NAMMEKIEVV PRVFDILYLD GETLMDRPLA
360 370 380 390 400
ERRRILEEVL DAHIAPQFLV TDVAGAEAIY TEALNLGHEG VMVKVPSSPY
410 420 430 440 450
TPGVRGRLWV KVKPGVETLD LAVIGAEWGE GRRAKMFGSF LLAVQDQGRL
460 470 480 490 500
LPVGKVATGI TDEVLADLYA LLKDSVIARS GKEVTLEPKV VFEVGYSEIQ
510 520 530 540
ASPNYASGYA LRFPRFVQVR EDKSVDEVET LDALAERYGE QKNGQGSL
Length:548
Mass (Da):61,152
Last modified:October 3, 2012 - v1
Checksum:iCEA0573FE66125EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE964772 Genomic DNA. Translation: CCJ35449.1.
RefSeqiWP_014866426.1. NC_018227.2.
YP_006544165.1. NC_018227.2.

Genome annotation databases

GeneIDi13354133.
KEGGimbg:BN140_0526.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE964772 Genomic DNA. Translation: CCJ35449.1.
RefSeqiWP_014866426.1. NC_018227.2.
YP_006544165.1. NC_018227.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13354133.
KEGGimbg:BN140_0526.

Phylogenomic databases

KOiK10747.
OMAiDAEVDIC.

Enzyme and pathway databases

BioCyciMBOU1201294:GLGD-538-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the hydrogenotrophic, methanogenic archaeon Methanoculleus bourgensis strain MS2T, isolated from a sewage sludge digester."
    Maus I., Wibberg D., Stantscheff R., Eikmeyer F.G., Seffner A., Boelter J., Szczepanowski R., Blom J., Jaenicke S., Konig H., Puhler A., Schluter A.
    J. Bacteriol. 194:5487-5488(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43281 / DSM 3045 / OCM 15 / MS2Imported.

Entry informationi

Entry nameiI7LLJ8_METBM
AccessioniPrimary (citable) accession number: I7LLJ8
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: July 22, 2015
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.