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Protein

Phosphoenolpyruvate carboxylase

Gene

ppcA

Organism
Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.UniRule annotation

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. phosphoenolpyruvate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbon fixation Source: UniProtKB-HAMAP
  2. oxaloacetate metabolic process Source: UniProtKB-HAMAP
  3. tricarboxylic acid cycle Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation

Enzyme and pathway databases

BioCyciMBOU1201294:GLGD-1492-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxylaseUniRule annotation (EC:4.1.1.31UniRule annotation)
Short name:
PEPCUniRule annotation
Short name:
PEPCaseUniRule annotation
Gene namesi
Name:ppcAUniRule annotationImported
Ordered Locus Names:BN140_1458Imported
OrganismiMethanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)Imported
Taxonomic identifieri1201294 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
ProteomesiUP000009007 Componenti: Chromosome

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the PEPCase type 2 family.UniRule annotation

Phylogenomic databases

KOiK01595.

Family and domain databases

HAMAPiMF_01904. PEPcase_type2.
InterProiIPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF14010. PEPcase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006677. UCP006677. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

I7KCZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRIPQCMST QHPDNVNLPF FAESPELGGE DEVQEAYYAY SHLGCTEQMW
60 70 80 90 100
DCEGKEVDNF VVRKLLSRYD AFFREKRLGR DVFLTLRVPN PEVETAEAKI
110 120 130 140 150
LLETLESIPR SYDTACLFYG DECPPIFEVI LPMTASHTAI DNIYRYYTDI
160 170 180 190 200
VVGKQERSLG EGGMTIADWI GRFRPDRINV IPLFEEMDHM LDAHNAVRRY
210 220 230 240 250
LEGKDITHQR VFLARSDPAM NYGIVSAVLL NKIALQHLQE VSEETGTGIY
260 270 280 290 300
PIIGVGSAPF RGGLSPRTVD QVTAEYPSVH TFTVQSAFKY DYPLEEVQNA
310 320 330 340 350
VRRLEERRPG RPRPVDEAAA LDLIERYTRA YERRLGPLAP LINRVARYVP
360 370 380 390 400
ERRKRKLHIG LFGYARSTGG ITLPRAIKFT AALYSVGFPP EILGLDALDD
410 420 430 440 450
TDIGFVRETY VNFDENLKAA ARYLNTETGL VPRELAARVA DLVDIEPDEE
460 470 480
HAEVTAEIAR ALRENDIGKL SALVLQAAHC RKFLG
Length:485
Mass (Da):54,857
Last modified:October 3, 2012 - v1
Checksum:i0369FAFE317A5C0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE964772 Genomic DNA. Translation: CCJ36381.1.
RefSeqiYP_006545097.1. NC_018227.2.

Genome annotation databases

GeneIDi13354835.
KEGGimbg:BN140_1458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE964772 Genomic DNA. Translation: CCJ36381.1.
RefSeqiYP_006545097.1. NC_018227.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13354835.
KEGGimbg:BN140_1458.

Phylogenomic databases

KOiK01595.

Enzyme and pathway databases

BioCyciMBOU1201294:GLGD-1492-MONOMER.

Family and domain databases

HAMAPiMF_01904. PEPcase_type2.
InterProiIPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF14010. PEPcase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006677. UCP006677. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the hydrogenotrophic, methanogenic archaeon Methanoculleus bourgensis strain MS2T, isolated from a sewage sludge digester."
    Maus I., Wibberg D., Stantscheff R., Eikmeyer F.G., Seffner A., Boelter J., Szczepanowski R., Blom J., Jaenicke S., Konig H., Puhler A., Schluter A.
    J. Bacteriol. 194:5487-5488(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43281 / DSM 3045 / OCM 15 / MS2Imported.

Entry informationi

Entry nameiI7KCZ4_METBM
AccessioniPrimary (citable) accession number: I7KCZ4
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: April 29, 2015
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.