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Protein

DNA ligase

Gene

lig3

Organism
Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei249 – 2491ATPUniRule annotation
Active sitei251 – 2511N6-AMP-lysine intermediateUniRule annotation
Binding sitei256 – 2561ATPUniRule annotation
Binding sitei271 – 2711ATPUniRule annotation
Binding sitei301 – 3011ATPUniRule annotation
Binding sitei341 – 3411ATPUniRule annotation
Binding sitei418 – 4181ATPUniRule annotation
Binding sitei424 – 4241ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBOU1201294:GLGD-645-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:lig3Imported
Synonyms:ligUniRule annotation
Ordered Locus Names:BN140_0625Imported
OrganismiMethanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 / MS2) (Methanogenium bourgense)Imported
Taxonomic identifieri1201294 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
ProteomesiUP000009007: Chromosome

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I7J7N3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFATLASCF ERLNVTSSRN DMTALLARLL AGASPDEIGI VCYFTLGAIA
60 70 80 90 100
PGYSGLTPGI GERTAAAAIA LAGSVDPAAV EAAARELGDY GDAAARLTVA
110 120 130 140 150
PPEGSTGPLR VTDVHRGFME IARASGPGSA DRKTRVLAAM LAAATPPERR
160 170 180 190 200
YLVRLATGEM RLGVGDMTLL DALAEAFLGS KAERPPLEHA YNISSDVGLV
210 220 230 240 250
ARTLQESGLA GVSAIPVTLH HPIRPMLTQR VSRISEIPER IGSRVIAVEE
260 270 280 290 300
KYDGERVQAH KDGDDVTLFS RRLTDVTHQY PDIVRHIRRC VTADTAILDG
310 320 330 340 350
EAVAFDHETG TYRPFQTLMR RRRKHRVREV AGEIPATYRV FDLLYVNGES
360 370 380 390 400
YLRRSYPDRR ARLEEVLSPD EKISPADRTL TGDLDGITEF YLACIERGLE
410 420 430 440 450
GVVCKSCAED SFYRAGAREW QWIKWKSDYG TELADTLDLV VVGANAGRGK
460 470 480 490 500
RAGTYGSVLC AAYNHEEDIF QTVCGMGTGF TDADLADLPR RFAGVRVDRQ
510 520 530 540 550
PARVMVTPQA VPDFWFAPAR VVEVLGLEIT ESPVHTCNWD PEWRRGLALR
560 570 580
FPRFVRWRDE KSPEAATTTA EVAAMFRRRR EG
Length:582
Mass (Da):63,967
Last modified:October 3, 2012 - v1
Checksum:i00CDF4CBE0E0262C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE964772 Genomic DNA. Translation: CCJ35548.1.
RefSeqiYP_006544264.1. NC_018227.2.

Genome annotation databases

GeneIDi13354285.
KEGGimbg:BN140_0625.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE964772 Genomic DNA. Translation: CCJ35548.1.
RefSeqiYP_006544264.1. NC_018227.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13354285.
KEGGimbg:BN140_0625.

Phylogenomic databases

KOiK10747.

Enzyme and pathway databases

BioCyciMBOU1201294:GLGD-645-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the hydrogenotrophic, methanogenic archaeon Methanoculleus bourgensis strain MS2T, isolated from a sewage sludge digester."
    Maus I., Wibberg D., Stantscheff R., Eikmeyer F.G., Seffner A., Boelter J., Szczepanowski R., Blom J., Jaenicke S., Konig H., Puhler A., Schluter A.
    J. Bacteriol. 194:5487-5488(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43281 / DSM 3045 / OCM 15 / MS2Imported.

Entry informationi

Entry nameiI7J7N3_METBM
AccessioniPrimary (citable) accession number: I7J7N3
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: February 4, 2015
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.