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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Natrinema sp. (strain J7-2)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei157Proton acceptorUniRule annotation1
Binding sitei159SubstrateUniRule annotation1
Metal bindingi183Magnesium; via carbamate groupUniRule annotation1
Metal bindingi185MagnesiumUniRule annotation1
Metal bindingi186MagnesiumUniRule annotation1
Active sitei275Proton acceptorUniRule annotation1
Binding sitei276SubstrateUniRule annotation1
Binding sitei308SubstrateUniRule annotation1
Sitei316Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:NJ7G_0819Imported
OrganismiNatrinema sp. (strain J7-2)Imported
Taxonomic identifieri406552 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaNatrialbalesNatrialbaceaeNatrinema
Proteomesi
  • UP000006507 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei183N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliI7CQ19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 125RuBisCO_large_NInterPro annotationAdd BLAST113
Domaini139 – 415RuBisCO_largeInterPro annotationAdd BLAST277

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni344 – 346Substrate bindingUniRule annotation3
Regioni366 – 369Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

KOiK01601.
OMAiFTQDWAS.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

Sequencei

Sequence statusi: Complete.

I7CQ19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDGIMTGIE YDDFLDLGYE PAGTELVCEF TIEPAAGMSM EAAASRVASE
60 70 80 90 100
SSNGTWAALH VDEDELTDLG AVACGIDGRT VTVAYPTALF EAGSMPQLLS
110 120 130 140 150
CIAGNILGMK AVDSIRLEDC DWPESITSGF PGPQFGPSVA REKLDAGERP
160 170 180 190 200
VLATVPKPKV GLSTDAHARI GEEAWRGGVD LLKDDENLTD QAFNPFADRL
210 220 230 240 250
AESLAARDRA QEETGERKDY LVNVTAETTE MLERVDLVAE HGGGFVMVDV
260 270 280 290 300
ITCGWAAVQS VRERCEDHGL AIHAHRAMHA AFDRLDHHGV SMRVIAQIAR
310 320 330 340 350
LCGVDHIHTG TAGLGKLANE DTPGINEWLT SDCHGLRPVL PVASGGLHPG
360 370 380 390 400
VVDQLIDALG TDIIVQAGGG IHGHPDGTHA GAKALRQSVE ASMAGTDLET
410 420
YADDHAELAT ALEKWGAETP R
Length:421
Mass (Da):44,615
Last modified:October 3, 2012 - v1
Checksum:i948B35B16AA05268
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003412 Genomic DNA. Translation: AFO56068.1.

Genome annotation databases

EnsemblBacteriaiAFO56068; AFO56068; NJ7G_0819.
KEGGinat:NJ7G_0819.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003412 Genomic DNA. Translation: AFO56068.1.

3D structure databases

ProteinModelPortaliI7CQ19.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFO56068; AFO56068; NJ7G_0819.
KEGGinat:NJ7G_0819.

Phylogenomic databases

KOiK01601.
OMAiFTQDWAS.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiI7CQ19_NATSJ
AccessioniPrimary (citable) accession number: I7CQ19
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.