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I7CQ19

- I7CQ19_NATSJ

UniProt

I7CQ19 - I7CQ19_NATSJ

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Natrinema sp. (strain J7-2)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 15 (01 Oct 2014)
      Sequence version 1 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei157 – 1571Proton acceptorUniRule annotation
    Binding sitei159 – 1591SubstrateUniRule annotation
    Metal bindingi183 – 1831Magnesium; via carbamate groupUniRule annotation
    Metal bindingi185 – 1851MagnesiumUniRule annotation
    Metal bindingi186 – 1861MagnesiumUniRule annotation
    Active sitei275 – 2751Proton acceptorUniRule annotation
    Binding sitei276 – 2761SubstrateUniRule annotation
    Binding sitei308 – 3081SubstrateUniRule annotation
    Sitei316 – 3161Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: InterPro

    Keywords - Molecular functioni

    LyaseUniRule annotation, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciNSP406552:GLHM-827-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotation
    Ordered Locus Names:NJ7G_0819Imported
    OrganismiNatrinema sp. (strain J7-2)Imported
    Taxonomic identifieri406552 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatrinema
    ProteomesiUP000006507: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei183 – 1831N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliI7CQ19.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni344 – 3463Substrate bindingUniRule annotation
    Regioni366 – 3694Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family.UniRule annotation
    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    KOiK01601.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProiIPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    I7CQ19-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDGIMTGIE YDDFLDLGYE PAGTELVCEF TIEPAAGMSM EAAASRVASE    50
    SSNGTWAALH VDEDELTDLG AVACGIDGRT VTVAYPTALF EAGSMPQLLS 100
    CIAGNILGMK AVDSIRLEDC DWPESITSGF PGPQFGPSVA REKLDAGERP 150
    VLATVPKPKV GLSTDAHARI GEEAWRGGVD LLKDDENLTD QAFNPFADRL 200
    AESLAARDRA QEETGERKDY LVNVTAETTE MLERVDLVAE HGGGFVMVDV 250
    ITCGWAAVQS VRERCEDHGL AIHAHRAMHA AFDRLDHHGV SMRVIAQIAR 300
    LCGVDHIHTG TAGLGKLANE DTPGINEWLT SDCHGLRPVL PVASGGLHPG 350
    VVDQLIDALG TDIIVQAGGG IHGHPDGTHA GAKALRQSVE ASMAGTDLET 400
    YADDHAELAT ALEKWGAETP R 421
    Length:421
    Mass (Da):44,615
    Last modified:October 3, 2012 - v1
    Checksum:i948B35B16AA05268
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003412 Genomic DNA. Translation: AFO56068.1.
    RefSeqiWP_014863184.1. NC_018224.1.
    YP_006540144.1. NC_018224.1.

    Genome annotation databases

    EnsemblBacteriaiAFO56068; AFO56068; NJ7G_0819.
    GeneIDi13350656.
    KEGGinat:NJ7G_0819.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003412 Genomic DNA. Translation: AFO56068.1 .
    RefSeqi WP_014863184.1. NC_018224.1.
    YP_006540144.1. NC_018224.1.

    3D structure databases

    ProteinModelPortali I7CQ19.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AFO56068 ; AFO56068 ; NJ7G_0819 .
    GeneIDi 13350656.
    KEGGi nat:NJ7G_0819.

    Phylogenomic databases

    KOi K01601.

    Enzyme and pathway databases

    BioCyci NSP406552:GLHM-827-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProi IPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Natrinema sp. J7-2, a haloarchaeon capable of growth on synthetic media without amino acid supplements."
      Feng J., Liu B., Zhang Z., Ren Y., Li Y., Gan F., Huang Y., Chen X., Shen P., Wang L., Tang B., Tang X.F.
      PLoS ONE 7:E41621-E41621(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: J7-2Imported.

    Entry informationi

    Entry nameiI7CQ19_NATSJ
    AccessioniPrimary (citable) accession number: I7CQ19
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 3, 2012
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 15 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3