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I7C1A7 (I7C1A7_PSEPT) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AFO50187.1
Ordered Locus Names:T1E_4358 EMBL AFO50187.1
OrganismPseudomonas putida (strain DOT-T1E) [Complete proteome] [HAMAP] EMBL AFO50187.1
Taxonomic identifier1196325 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain116 – 311196ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding142 – 19756ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2651Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2781Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2781Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2801Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I7C1A7 [UniParc].

Last modified October 3, 2012. Version 1.
Checksum: 83499F4D6AE6FEB7

FASTA31834,022
        10         20         30         40         50         60 
MTSAYDKLQS TLDVKAFGRV AVLYGGKSAE REVSLKSGAA VIDALSTAGV DVVGIDVGDD 

        70         80         90        100        110        120 
LLARLQSEKI DRAFIILHGR GGEDGSMQGL LECLGIPYTG SGILASALAM DKLRTKQVWQ 

       130        140        150        160        170        180 
SLGIPTPRHA VLASESDCLQ ASTELGFPLI VKPAHEGSSI GMAKVNSAQE LVAAWQDAAK 

       190        200        210        220        230        240 
YDSQVLVEQW IHGPEFTIAV LRGQVLPPIA LGTPHVFYDY DAKYIANDTQ YRIPCGLDSV 

       250        260        270        280        290        300 
KEQELIDLTA RACDAIGIEG WGRLDVMQDE QGQFWLLEVN TAPGMTDHSL VPMAARAAGL 

       310 
DFQQLVLAIL AESVATRG 

« Hide

References

[1]"Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome."
Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A., Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.
Microb. Biotechnol. 6:598-611(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DOT-T1E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003734 Genomic DNA. Translation: AFO50187.1.
RefSeqYP_006534981.1. NC_018220.1.

3D structure databases

ProteinModelPortalI7C1A7.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFO50187; AFO50187; T1E_4358.
GeneID13343301.
KEGGppx:T1E_4358.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycPPUT1196325:GLIV-4384-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI7C1A7_PSEPT
AccessionPrimary (citable) accession number: I7C1A7
Entry history
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: February 19, 2014
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)