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I7C1A7

- I7C1A7_PSEPT

UniProt

I7C1A7 - I7C1A7_PSEPT

Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Pseudomonas putida (strain DOT-T1E)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 14 (01 Oct 2014)
      Sequence version 1 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Cell wall formation.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi265 – 2651Magnesium or manganese 1UniRule annotation
    Metal bindingi278 – 2781Magnesium or manganese 1UniRule annotation
    Metal bindingi278 – 2781Magnesium or manganese 2UniRule annotation
    Metal bindingi280 – 2801Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi142 – 19756ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. D-alanine-D-alanine ligase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    2. regulation of cell shape Source: UniProtKB-KW

    Keywords - Molecular functioni

    LigaseUniRule annotationSAAS annotationImported

    Keywords - Biological processi

    Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPPUT1196325:GLIV-4384-MONOMER.
    UniPathwayiUPA00219.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
    Alternative name(s):
    D-Ala-D-Ala ligaseUniRule annotation
    D-alanylalanine synthetaseUniRule annotation
    Gene namesi
    Name:ddlUniRule annotationImported
    Ordered Locus Names:T1E_4358Imported
    OrganismiPseudomonas putida (strain DOT-T1E)Imported
    Taxonomic identifieri1196325 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000006503: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliI7C1A7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini116 – 311196ATP-graspUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the D-alanine--D-alanine ligase family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation
    Contains ATP-grasp domain.SAAS annotation

    Phylogenomic databases

    KOiK01921.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    HAMAPiMF_00047. Dala_Dala_lig.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR23132. PTHR23132. 1 hit.
    PfamiPF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    PS00844. DALA_DALA_LIGASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    I7C1A7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSAYDKLQS TLDVKAFGRV AVLYGGKSAE REVSLKSGAA VIDALSTAGV    50
    DVVGIDVGDD LLARLQSEKI DRAFIILHGR GGEDGSMQGL LECLGIPYTG 100
    SGILASALAM DKLRTKQVWQ SLGIPTPRHA VLASESDCLQ ASTELGFPLI 150
    VKPAHEGSSI GMAKVNSAQE LVAAWQDAAK YDSQVLVEQW IHGPEFTIAV 200
    LRGQVLPPIA LGTPHVFYDY DAKYIANDTQ YRIPCGLDSV KEQELIDLTA 250
    RACDAIGIEG WGRLDVMQDE QGQFWLLEVN TAPGMTDHSL VPMAARAAGL 300
    DFQQLVLAIL AESVATRG 318
    Length:318
    Mass (Da):34,022
    Last modified:October 3, 2012 - v1
    Checksum:i83499F4D6AE6FEB7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003734 Genomic DNA. Translation: AFO50187.1.
    RefSeqiYP_006534981.1. NC_018220.1.

    Genome annotation databases

    EnsemblBacteriaiAFO50187; AFO50187; T1E_4358.
    GeneIDi13343301.
    KEGGippx:T1E_4358.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003734 Genomic DNA. Translation: AFO50187.1 .
    RefSeqi YP_006534981.1. NC_018220.1.

    3D structure databases

    ProteinModelPortali I7C1A7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AFO50187 ; AFO50187 ; T1E_4358 .
    GeneIDi 13343301.
    KEGGi ppx:T1E_4358.

    Phylogenomic databases

    KOi K01921.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .
    BioCyci PPUT1196325:GLIV-4384-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    HAMAPi MF_00047. Dala_Dala_lig.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR23132. PTHR23132. 1 hit.
    Pfami PF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    PS00844. DALA_DALA_LIGASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome."
      Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A., Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.
      Microb. Biotechnol. 6:598-611(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DOT-T1EImported.

    Entry informationi

    Entry nameiI7C1A7_PSEPT
    AccessioniPrimary (citable) accession number: I7C1A7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 3, 2012
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 14 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3