Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

I7AXN0 (I7AXN0_ENTFL) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
ORF Names:EFD32_0658 EMBL AFO43548.1
OrganismEnterococcus faecalis D32 [Complete proteome] EMBL AFO43548.1
Taxonomic identifier1206105 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain136 – 341206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2951Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3101Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I7AXN0 [UniParc].

Last modified October 3, 2012. Version 1.
Checksum: 38C9710F0967B20B

FASTA35640,392
        10         20         30         40         50         60 
MKIILLYGGR SEEHDVSVLS AYSVLNAIYY KYYQVQLVFI SKDGQWVKGP LLSERPQNKE 

        70         80         90        100        110        120 
VLHLTWAQTP EETGEFSGKR ISPSEIYEEE AIVFPVLHGP NGEDGTIQGF METINMPYVG 

       130        140        150        160        170        180 
AGVLASANAM DKIMTKYLLQ TVGIPQVPFV PVLRSDWKGN PKEVFEKCEG SLIYPVFVKP 

       190        200        210        220        230        240 
ANMGSSVGIS KAENREELQE ALEEAFRYDA RAIVEQGIEA REIEVAILGN EDVRTTLPGE 

       250        260        270        280        290        300 
VVKDVAFYDY DAKYINNTIE MQIPAHVPEE VAHQAQEYAK KAYIMLDGSG LSRCDFFLTS 

       310        320        330        340        350 
KNELFLNELN TMPGFTDFSM YPLLWENMGL KYSDLIEELI QLALNRFKQR QEFYNN 

« Hide

References

[1]"Complete Genome Sequence of the Porcine Isolate Enterococcus faecalis D32."
Zischka M., Kuenne C., Blom J., Dabrowski P.W., Linke B., Hain T., Nitsche A., Goesmann A., Larsen J., Jensen L.B., Witte W., Werner G.
J. Bacteriol. 194:5490-5491(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: D32 EMBL AFO43548.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003726 Genomic DNA. Translation: AFO43548.1.
RefSeqYP_006537025.1. NC_018221.1.

3D structure databases

ProteinModelPortalI7AXN0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFO43548; AFO43548; EFD32_0658.
GeneID13347158.
KEGGefd:EFD32_0658.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycEFAE1206105:GLCS-687-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI7AXN0_ENTFL
AccessionPrimary (citable) accession number: I7AXN0
Entry history
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: June 11, 2014
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)