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I7A4C8

- I7A4C8_PSEST

UniProt

I7A4C8 - I7A4C8_PSEST

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Protein

Elongation factor Tu

Gene

tuf

Organism
Pseudomonas stutzeri DSM 10701
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi83 – 875GTPUniRule annotation
Nucleotide bindingi138 – 1414GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-HAMAP
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factorUniRule annotation

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSTU1123519:GLIY-3422-MONOMER.
PSTU1123519:GLIY-3436-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
ORF Names:PSJM300_16935Imported, PSJM300_17000Imported
OrganismiPseudomonas stutzeri DSM 10701Imported
Taxonomic identifieri1123519 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000009004: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

KOiK02358.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I7A4C8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKEKFERNK PHVNVGTIGH VDHGKTTLTA ALTRVCAEVF GSGGVSKGYD
60 70 80 90 100
QIDNAPEEKA RGITINTSHV EYDSPIRHYA HVDCPGHADY VKNMITGAAQ
110 120 130 140 150
MDGAILVCSA ADGPMPQTRE HILLSRQVGV PYIVVFLNKA DMVDDAELLE
160 170 180 190 200
LVEMEVRDLL STYDFPGDDT PIIIGSALMA LNGEDENGLG TSAVKKLVET
210 220 230 240 250
LDSYIPEPER AIDKPFLMPI EDVFSISGRG TVVTGRVERG IVRVQEEIEI
260 270 280 290 300
VGIRDTTKTT CTGVEMFRKL LDEGRAGENV GVLLRGTKRD DVERGQVLAK
310 320 330 340 350
PGTIKPHTKF EGEVYVLSKE EGGRHTPFFK GYRPQFYFRT TDVTGNCELP
360 370 380 390
EGVEMVMPGD NIKMVVTLIA PIAMEDGLRF AIREGGRTVG AGVVAKIIE
Length:399
Mass (Da):43,572
Last modified:October 3, 2012 - v1
Checksum:iB7381132E9B89029
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003725 Genomic DNA. Translation: AFN79444.1.
CP003725 Genomic DNA. Translation: AFN79457.1.
RefSeqiYP_006525802.1. NC_018177.1.
YP_006525815.1. NC_018177.1.

Genome annotation databases

EnsemblBacteriaiAFN79444; AFN79444; PSJM300_16935.
AFN79457; AFN79457; PSJM300_17000.
GeneIDi13325983.
13325995.
KEGGipsj:PSJM300_16935.
psj:PSJM300_17000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003725 Genomic DNA. Translation: AFN79444.1 .
CP003725 Genomic DNA. Translation: AFN79457.1 .
RefSeqi YP_006525802.1. NC_018177.1.
YP_006525815.1. NC_018177.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFN79444 ; AFN79444 ; PSJM300_16935 .
AFN79457 ; AFN79457 ; PSJM300_17000 .
GeneIDi 13325983.
13325995.
KEGGi psj:PSJM300_16935.
psj:PSJM300_17000.

Phylogenomic databases

KOi K02358.

Enzyme and pathway databases

BioCyci PSTU1123519:GLIY-3422-MONOMER.
PSTU1123519:GLIY-3436-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_00118_B. EF_Tu_B.
InterProi IPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS00301. EFACTOR_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome Sequence of Pseudomonas stutzeri Strain JM300 (DSM 10701), a Soil Isolate and Model Organism for Natural Transformation."
    Busquets A., Pena A., Gomila M., Bosch R., Nogales B., Garcia-Valdes E., Lalucat J., Bennasar A.
    J. Bacteriol. 194:5477-5478(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 10701Imported.

Entry informationi

Entry nameiI7A4C8_PSEST
AccessioniPrimary (citable) accession number: I7A4C8
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: November 26, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3