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I6V1U3 (I6V1U3_9EURY) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase RuleBase RU000617 HAMAP-Rule MF_00407

EC=6.5.1.1 RuleBase RU000617 HAMAP-Rule MF_00407
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:lig HAMAP-Rule MF_00407
ORF Names:PFC_10430 EMBL AFN05005.1
OrganismPyrococcus furiosus COM1 [Complete proteome] EMBL AFN05005.1
Taxonomic identifier1185654 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. HAMAP-Rule MF_00407 RuleBase RU004196

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2491N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2471ATP By similarity HAMAP-Rule MF_00407
Binding site2541ATP By similarity HAMAP-Rule MF_00407
Binding site2691ATP By similarity HAMAP-Rule MF_00407
Binding site2991ATP By similarity HAMAP-Rule MF_00407
Binding site3391ATP By similarity HAMAP-Rule MF_00407
Binding site4141ATP By similarity HAMAP-Rule MF_00407
Binding site4201ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
I6V1U3 [UniParc].

Last modified October 3, 2012. Version 1.
Checksum: 534158525B9D24B2

FASTA56163,773
        10         20         30         40         50         60 
MRYLELAQLY QKLEKTTMKL IKTRLVADFL KKVPDDHLEF IPYLILGEVF PEWDERELGV 

        70         80         90        100        110        120 
GEKLLIKAVA MATGIDAKEI EESVKDTGDL GESIALAVKK KKQKSFFSQP LTIKRVYQTL 

       130        140        150        160        170        180 
VKVAETTGEG SQDKKVKYLA DLFMDAEPLE AKYLARTILG TMRTGVAEGL LRDAIAMAFH 

       190        200        210        220        230        240 
VKVELVERAY MLTSDFGYVA KIAKLEGNEG LAKVQVQLGK PIKPMLAQQA ASIRDALLEM 

       250        260        270        280        290        300 
GGEAEFEIKY DGARVQVHKD GSKIIVYSRR LENVTRAIPE IVEALKEAII PEKAIVEGEL 

       310        320        330        340        350        360 
VAIGENGRPL PFQYVLRRFR RKHNIEEMME KIPLELNLFD VLYVDGQSLI DTKFIDRRRT 

       370        380        390        400        410        420 
LEEIIKQNEK IKVAENLITK KVEEAEAFYK RALEMGHEGL MAKRLDAVYE PGNRGKKWLK 

       430        440        450        460        470        480 
IKPTMENLDL VIIGAEWGEG RRAHLFGSFI LGAYDPETGE FLEVGKVGSG FTDDDLVEFT 

       490        500        510        520        530        540 
KMLKPLIIKE EGKRVWLQPK VVIEVTYQEI QKSPKYRSGF ALRFPRFVAL RDDKGPEDAD 

       550        560 
TIERIAQLYE LQEKMKGKVE S 

« Hide

References

[1]"Genome Sequencing of a Genetically-Tractable Pyrococcus furiosus Strain Reveals a Highly Dynamic Genome."
Bridger S.L., Lancaster W.A., Poole F.L.II., Schut G.J., Adams M.W.
J. Bacteriol. 194:4097-4106(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: COM1 EMBL AFN05005.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003685 Genomic DNA. Translation: AFN05005.1.
RefSeqYP_006493297.1. NC_018092.1.

3D structure databases

ProteinModelPortalI6V1U3.
SMRI6V1U3. Positions 1-561.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFN05005; AFN05005; PFC_10430.
GeneID13300654.
KEGGpfi:PFC_10430.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK10747.

Enzyme and pathway databases

BioCycPFUR1185654:GLJ1-2078-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI6V1U3_9EURY
AccessionPrimary (citable) accession number: I6V1U3
Entry history
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: February 19, 2014
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)