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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Pyrococcus furiosus COM1
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551Proton acceptorUniRule annotation
Binding sitei157 – 1571SubstrateUniRule annotation
Metal bindingi181 – 1811Magnesium; via carbamate groupUniRule annotation
Metal bindingi183 – 1831MagnesiumUniRule annotation
Metal bindingi184 – 1841MagnesiumUniRule annotation
Active sitei273 – 2731Proton acceptorUniRule annotation
Binding sitei274 – 2741SubstrateUniRule annotation
Binding sitei306 – 3061SubstrateUniRule annotation
Sitei313 – 3131Transition state stabilizerUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. oxidoreductase activity Source: UniProtKB-KW
  3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. AMP catabolic process Source: UniProtKB-HAMAP
  2. carbon fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciPFUR1185654:GLJ1-992-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotationImported
ORF Names:PFC_05005Imported
OrganismiPyrococcus furiosus COM1Imported
Taxonomic identifieri1185654 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000006216 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811N6-carboxylysineUniRule annotation

Proteomic databases

PRIDEiI6V1K5.

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliI6V1K5.
SMRiI6V1K5. Positions 1-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni343 – 3453Substrate bindingUniRule annotation
Regioni365 – 3684Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

KOiK01601.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3.
InterProiIPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

Sequencei

Sequence statusi: Complete.

I6V1K5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVEWYLDFV DLDYTPGRDE LIVEYYFEPN GVSPEEAAGR IASESSIGTW
60 70 80 90 100
TTLWKMPEMA KRSMAKVFYL EKSGEGYIAK IAYPLTLFEE GSIVQLLSAI
110 120 130 140 150
AGNIFGMKAL KNLRLLDFHP PYEYLRHFKG PQYGVKGIRE FMGVKERPLT
160 170 180 190 200
ATVPKPKMGW SVDEYAEIAY ELWSGGIDLL KDDENFTSFP FNRFEERVKK
210 220 230 240 250
LYRIRDIVEA ETEERKEYLI NITGSVDVME KRAELVANEG GQYVMIDIIV
260 270 280 290 300
TGWSALQYMR EVTEDLGLAI HAHRAMHAAF TRNPKHGITM YAIAKLARMI
310 320 330 340 350
GVDQIHTGTA VGKMAGDYEE VKRINDFLLS KWEHIREVFP VASGGLHPGL
360 370 380 390 400
MPELIRLFGK DLVIQAGGGV MGHPDGPRAG AKALRDAIDA ALEGVDLEEK
410 420
AKSSPELKKA LDKWGYLKPK
Length:420
Mass (Da):47,348
Last modified:October 3, 2012 - v1
Checksum:i0271D055DCFF3DC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003685 Genomic DNA. Translation: AFN03948.1.
RefSeqiYP_006492240.1. NC_018092.1.

Genome annotation databases

EnsemblBacteriaiAFN03948; AFN03948; PFC_05005.
GeneIDi13301761.
KEGGipfi:PFC_05005.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003685 Genomic DNA. Translation: AFN03948.1.
RefSeqiYP_006492240.1. NC_018092.1.

3D structure databases

ProteinModelPortaliI6V1K5.
SMRiI6V1K5. Positions 1-411.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiI6V1K5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFN03948; AFN03948; PFC_05005.
GeneIDi13301761.
KEGGipfi:PFC_05005.

Phylogenomic databases

KOiK01601.

Enzyme and pathway databases

BioCyciPFUR1185654:GLJ1-992-MONOMER.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3.
InterProiIPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome Sequencing of a Genetically-Tractable Pyrococcus furiosus Strain Reveals a Highly Dynamic Genome."
    Bridger S.L., Lancaster W.A., Poole F.L.II., Schut G.J., Adams M.W.
    J. Bacteriol. 194:4097-4106(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: COM1Imported.

Entry informationi

Entry nameiI6V1K5_9EURY
AccessioniPrimary (citable) accession number: I6V1K5
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: April 29, 2015
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.