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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Pyrococcus furiosus COM1
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Proton acceptorUniRule annotation1
Binding sitei157SubstrateUniRule annotation1
Metal bindingi181Magnesium; via carbamate groupUniRule annotation1
Metal bindingi183MagnesiumUniRule annotation1
Metal bindingi184MagnesiumUniRule annotation1
Active sitei273Proton acceptorUniRule annotation1
Binding sitei274SubstrateUniRule annotation1
Binding sitei306SubstrateUniRule annotation1
Sitei313Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotationImported
ORF Names:PFC_05005Imported
OrganismiPyrococcus furiosus COM1Imported
Taxonomic identifieri1185654 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000006216 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliI6V1K5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 124RuBisCO_large_NInterPro annotationAdd BLAST117
Domaini134 – 414RuBisCO_largeInterPro annotationAdd BLAST281

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni343 – 345Substrate bindingUniRule annotation3
Regioni365 – 368Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

KOiK01601.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

Sequencei

Sequence statusi: Complete.

I6V1K5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVEWYLDFV DLDYTPGRDE LIVEYYFEPN GVSPEEAAGR IASESSIGTW
60 70 80 90 100
TTLWKMPEMA KRSMAKVFYL EKSGEGYIAK IAYPLTLFEE GSIVQLLSAI
110 120 130 140 150
AGNIFGMKAL KNLRLLDFHP PYEYLRHFKG PQYGVKGIRE FMGVKERPLT
160 170 180 190 200
ATVPKPKMGW SVDEYAEIAY ELWSGGIDLL KDDENFTSFP FNRFEERVKK
210 220 230 240 250
LYRIRDIVEA ETEERKEYLI NITGSVDVME KRAELVANEG GQYVMIDIIV
260 270 280 290 300
TGWSALQYMR EVTEDLGLAI HAHRAMHAAF TRNPKHGITM YAIAKLARMI
310 320 330 340 350
GVDQIHTGTA VGKMAGDYEE VKRINDFLLS KWEHIREVFP VASGGLHPGL
360 370 380 390 400
MPELIRLFGK DLVIQAGGGV MGHPDGPRAG AKALRDAIDA ALEGVDLEEK
410 420
AKSSPELKKA LDKWGYLKPK
Length:420
Mass (Da):47,348
Last modified:October 3, 2012 - v1
Checksum:i0271D055DCFF3DC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003685 Genomic DNA. Translation: AFN03948.1.
RefSeqiWP_011012296.1. NC_018092.1.

Genome annotation databases

EnsemblBacteriaiAFN03948; AFN03948; PFC_05005.
GeneIDi1469025.
KEGGipfi:PFC_05005.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003685 Genomic DNA. Translation: AFN03948.1.
RefSeqiWP_011012296.1. NC_018092.1.

3D structure databases

ProteinModelPortaliI6V1K5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFN03948; AFN03948; PFC_05005.
GeneIDi1469025.
KEGGipfi:PFC_05005.

Phylogenomic databases

KOiK01601.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiI6V1K5_9EURY
AccessioniPrimary (citable) accession number: I6V1K5
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.