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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Pyrococcus furiosus COM1
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Proton acceptorUniRule annotation1
Binding sitei157SubstrateUniRule annotation1
Metal bindingi181Magnesium; via carbamate groupUniRule annotation1
Metal bindingi183MagnesiumUniRule annotation1
Metal bindingi184MagnesiumUniRule annotation1
Active sitei273Proton acceptorUniRule annotation1
Binding sitei274SubstrateUniRule annotation1
Binding sitei306SubstrateUniRule annotation1
Sitei313Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationImported, OxidoreductaseUniRule annotation
Biological processCarbon dioxide fixationUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotationImported
ORF Names:PFC_05005Imported
OrganismiPyrococcus furiosus COM1Imported
Taxonomic identifieri1185654 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181N6-carboxylysineUniRule annotation1

Proteomic databases

PRIDEiI6V1K5

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliI6V1K5
SMRiI6V1K5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 124RuBisCO_large_NInterPro annotationAdd BLAST117
Domaini134 – 414RuBisCO_largeInterPro annotationAdd BLAST281

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni343 – 345Substrate bindingUniRule annotation3
Regioni365 – 368Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

KOiK01601
OrthoDBiPOG093Z02MJ

Family and domain databases

CDDicd08213 RuBisCO_large_III, 1 hit
Gene3Di3.20.20.110, 1 hit
3.30.70.150, 1 hit
HAMAPiMF_01133 RuBisCO_L_type3, 1 hit
InterProiView protein in InterPro
IPR033966 RuBisCO
IPR017712 RuBisCO_III
IPR000685 RuBisCO_lsu_C
IPR036376 RuBisCO_lsu_C_sf
IPR017443 RuBisCO_lsu_fd_N
IPR036422 RuBisCO_lsu_N_sf
PfamiView protein in Pfam
PF00016 RuBisCO_large, 1 hit
PF02788 RuBisCO_large_N, 1 hit
SFLDiSFLDS00014 RuBisCO, 1 hit
SUPFAMiSSF51649 SSF51649, 1 hit
SSF54966 SSF54966, 1 hit
TIGRFAMsiTIGR03326 rubisco_III, 1 hit

Sequencei

Sequence statusi: Complete.

I6V1K5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVEWYLDFV DLDYTPGRDE LIVEYYFEPN GVSPEEAAGR IASESSIGTW
60 70 80 90 100
TTLWKMPEMA KRSMAKVFYL EKSGEGYIAK IAYPLTLFEE GSIVQLLSAI
110 120 130 140 150
AGNIFGMKAL KNLRLLDFHP PYEYLRHFKG PQYGVKGIRE FMGVKERPLT
160 170 180 190 200
ATVPKPKMGW SVDEYAEIAY ELWSGGIDLL KDDENFTSFP FNRFEERVKK
210 220 230 240 250
LYRIRDIVEA ETEERKEYLI NITGSVDVME KRAELVANEG GQYVMIDIIV
260 270 280 290 300
TGWSALQYMR EVTEDLGLAI HAHRAMHAAF TRNPKHGITM YAIAKLARMI
310 320 330 340 350
GVDQIHTGTA VGKMAGDYEE VKRINDFLLS KWEHIREVFP VASGGLHPGL
360 370 380 390 400
MPELIRLFGK DLVIQAGGGV MGHPDGPRAG AKALRDAIDA ALEGVDLEEK
410 420
AKSSPELKKA LDKWGYLKPK
Length:420
Mass (Da):47,348
Last modified:October 3, 2012 - v1
Checksum:i0271D055DCFF3DC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003685 Genomic DNA Translation: AFN03948.1
RefSeqiWP_011012296.1, NC_018092.1

Genome annotation databases

EnsemblBacteriaiAFN03948; AFN03948; PFC_05005
GeneIDi1469025
KEGGipfi:PFC_05005
PATRICifig|1185654.4.peg.1020

Similar proteinsi

Entry informationi

Entry nameiI6V1K5_9EURY
AccessioniPrimary (citable) accession number: I6V1K5
Entry historyiIntegrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: May 23, 2018
This is version 38 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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