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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Pyrococcus furiosus COM1
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.UniRule annotationSAAS annotation

Pathway:iglycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotationSAAS annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (PFC_05160)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111NAD; via amide nitrogenUniRule annotation
Active sitei141 – 1411NucleophileUniRule annotation
Binding sitei167 – 1671NADUniRule annotation
Binding sitei298 – 2981NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 132NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotation

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

NADUniRule annotation, NADPUniRule annotation

Enzyme and pathway databases

BioCyciPFUR1185654:GLJ1-1833-MONOMER.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation (EC:1.2.1.59UniRule annotation)
Short name:
GAPDHUniRule annotation
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation
Gene namesi
Name:gapUniRule annotation
ORF Names:PFC_09215Imported
OrganismiPyrococcus furiosus COM1Imported
Taxonomic identifieri1185654 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000006216 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliI6URY1.
SMRiI6URY1. Positions 1-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 1423Glyceraldehyde 3-phosphate bindingUniRule annotation
Regioni192 – 1932Glyceraldehyde 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

KOiK00150.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I6URY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIKVGINGY GTIGKRVAYA VTKQDDMELI GVTKTKPDFE AYRAKELGIP
60 70 80 90 100
VYAASEEFLP RFEKAGFEVE GTLNDLLEKV DIIVDATPGG MGEKNKQLYE
110 120 130 140 150
KAGVKAIFQG GEKAEVAQVS FVAQANYEAA LGKDYVRVVS CNTTGLVRTL
160 170 180 190 200
NAIKDYVDYV YAVMIRRAAD PNDIKRGPIN AIKPSVTIPS HHGPDVQTVI
210 220 230 240 250
PINIETSAFV VPTTIMHVHS IMVELKKPLT REDVIDIFEN TTRVLLFEKE
260 270 280 290 300
KGFESTAQLI EFARDLHREW NNLYEIAVWK ESINVKGNRL FYIQAVHQES
310 320 330
DVIPENIDAI RAMFEIAEKW ESIKKTNKSL GILK
Length:334
Mass (Da):37,436
Last modified:October 3, 2012 - v1
Checksum:i224E0626923AFC66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003685 Genomic DNA. Translation: AFN04766.1.
RefSeqiWP_011013013.1. NC_018092.1.
YP_006493058.1. NC_018092.1.

Genome annotation databases

EnsemblBacteriaiAFN04766; AFN04766; PFC_09215.
GeneIDi13301306.
KEGGipfi:PFC_09215.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003685 Genomic DNA. Translation: AFN04766.1.
RefSeqiWP_011013013.1. NC_018092.1.
YP_006493058.1. NC_018092.1.

3D structure databases

ProteinModelPortaliI6URY1.
SMRiI6URY1. Positions 1-334.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFN04766; AFN04766; PFC_09215.
GeneIDi13301306.
KEGGipfi:PFC_09215.

Phylogenomic databases

KOiK00150.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
BioCyciPFUR1185654:GLJ1-1833-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome Sequencing of a Genetically-Tractable Pyrococcus furiosus Strain Reveals a Highly Dynamic Genome."
    Bridger S.L., Lancaster W.A., Poole F.L.II., Schut G.J., Adams M.W.
    J. Bacteriol. 194:4097-4106(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: COM1Imported.

Entry informationi

Entry nameiI6URY1_9EURY
AccessioniPrimary (citable) accession number: I6URY1
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: April 29, 2015
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.