SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

I6T1F7

- I6T1F7_ENTHA

UniProt

I6T1F7 - I6T1F7_ENTHA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA, EHR_14015, I584_01369, UAE_02067
Organism
Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogen By similarityUniRule annotation
Metal bindingi103 – 1031Magnesium; catalytic By similarityUniRule annotation
Active sitei127 – 1271Proton acceptor By similarityUniRule annotation
Binding sitei154 – 1541Allosteric activator ADP By similarityUniRule annotation
Binding sitei162 – 1621Substrate; shared with dimeric partner By similarityUniRule annotation
Binding sitei211 – 2111Allosteric activator ADP By similarityUniRule annotation
Binding sitei222 – 2221Substrate By similarityUniRule annotation
Binding sitei243 – 2431Substrate; shared with dimeric partner By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATP By similarityUniRule annotation
Nucleotide bindingi102 – 1054ATP By similarityUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciEHIR768486:GLCW-2747-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:EHR_14015Imported
ORF Names:I584_01369Imported, UAE_02067Imported
OrganismiEnterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)Imported
Taxonomic identifieri768486 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000002895: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni125 – 1273Substrate binding By similarityUniRule annotation
Regioni169 – 1713Substrate binding By similarityUniRule annotation
Regioni185 – 1873Allosteric activator ADP binding By similarityUniRule annotation
Regioni213 – 2153Allosteric activator ADP binding By similarityUniRule annotation
Regioni249 – 2524Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

KOiK00850.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

I6T1F7-1 [UniParc]FASTAAdd to Basket

« Hide

MKRIGILTSG GDAPGMNAAI RAVVRKGIYE GLEVYGINYG FAGLVAGDIR    50
RLDVADVGDK IQRGGTFLYS ARYPEFATEE GQLKGIEQLK KFGIEGLVVI 100
GGDGSYHGAM ALTRRGFPAV GVPGTIDNDI PGTDFTIGFD TAINTVLESI 150
DRIRDTATSH VRTFVIEVMG RNAGDIALWS GVAGGADEII IPEHDFDMAS 200
VAKKIQEGRD RGKKHCLIIL AEGVMGGNEF AEKLSEYGDY HTRVSILGHV 250
VRGGSPSARD RVLASKFGAY AVDLLRAGKG GLCVGIRDNE MVAADIIETL 300
ESNKHKPDLS LYDLNNSLSF 320
Length:320
Mass (Da):34,236
Last modified:October 3, 2012 - v1
Checksum:i6FCD34771774AFE2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003504 Genomic DNA. Translation: AFM71652.1.
AJAI01000018 Genomic DNA. Translation: EOH69574.1.
ASVZ01000002 Genomic DNA. Translation: EOU05472.1.
RefSeqiYP_006488543.1. NC_018081.1.

Genome annotation databases

EnsemblBacteriaiAFM71652; AFM71652; EHR_14015.
EOH69574; EOH69574; UAE_02067.
EOU05472; EOU05472; I584_01369.
GeneIDi13176784.
KEGGiehr:EHR_14015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003504 Genomic DNA. Translation: AFM71652.1 .
AJAI01000018 Genomic DNA. Translation: EOH69574.1 .
ASVZ01000002 Genomic DNA. Translation: EOU05472.1 .
RefSeqi YP_006488543.1. NC_018081.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFM71652 ; AFM71652 ; EHR_14015 .
EOH69574 ; EOH69574 ; UAE_02067 .
EOU05472 ; EOU05472 ; I584_01369 .
GeneIDi 13176784.
KEGGi ehr:EHR_14015.

Phylogenomic databases

KOi K00850.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci EHIR768486:GLCW-2747-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis."
    Gaechter T., Wunderlin C., Schmidheini T., Solioz M.
    J. Bacteriol. 194:5126-5127(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 9790Imported and ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
  2. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 9790Imported.
  3. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 9790Imported.

Entry informationi

Entry nameiI6T1F7_ENTHA
AccessioniPrimary (citable) accession number: I6T1F7
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: September 3, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome

External Data

Dasty 3

Similar proteinsi