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I6SPL1

- I6SPL1_ENTCL

UniProt

I6SPL1 - I6SPL1_ENTCL

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA, A3UG_22510
Organism
Enterobacter cloacae subsp. dissolvens SDM
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotationSAAS annotations

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP; via amide nitrogen By similarityUniRule annotation
Metal bindingi104 – 1041Magnesium; catalytic By similarityUniRule annotation
Active sitei128 – 1281Proton acceptor By similarityUniRule annotation
Binding sitei155 – 1551Allosteric activator ADP By similarityUniRule annotation
Binding sitei163 – 1631Substrate; shared with dimeric partner By similarityUniRule annotation
Binding sitei212 – 2121Allosteric activator ADP By similarityUniRule annotation
Binding sitei223 – 2231Substrate By similarityUniRule annotation
Binding sitei244 – 2441Substrate; shared with dimeric partner By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742ATP By similarityUniRule annotation
Nucleotide bindingi103 – 1064ATP By similarityUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkAUniRule annotation
ORF Names:A3UG_22510Imported
OrganismiEnterobacter cloacae subsp. dissolvens SDMImported
Taxonomic identifieri1104326 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
ProteomesiUP000002891: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotationSAAS annotations

Structurei

3D structure databases

ProteinModelPortaliI6SPL1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 265Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni55 – 606Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni126 – 1283Substrate binding By similarityUniRule annotation
Regioni170 – 1723Substrate binding By similarityUniRule annotation
Regioni186 – 1883Allosteric activator ADP binding By similarityUniRule annotation
Regioni214 – 2163Allosteric activator ADP binding By similarityUniRule annotation
Regioni250 – 2534Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

KOiK00850.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I6SPL1-1 [UniParc]FASTAAdd to Basket

« Hide

MIKKIGVLTS GGDAPGMNAA IRGVVRAALT EGLEVFGIYD GYLGLYEDRM    50
VQLDRYSVSD MINRGGTFLG SARFPEFRDE HVREVAIENM KKRGLDALVV 100
IGGDGSYMGA KRLTEMGFPC IGLPGTIDND IKGTDYTIGY FTALGTVVEA 150
IDRLRDTSSS HQRISIVEVM GRYCGDLTLA AAIAGGCEFV VVPEVEFSRE 200
DLVAEIKAGI AKGKKHAIVA ITEHICDVDE LAKYIEAETK RETRATVLGH 250
IQRGGSPGPY DRILASRMGA YAIELLLQGH GGRCVGIQNE KLVHHDIIDA 300
IENMKRPFKG DWLDCAKKLY 320
Length:320
Mass (Da):34,887
Last modified:October 3, 2012 - v1
Checksum:iFBB76628CFE6F10C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003678 Genomic DNA. Translation: AFM62201.1.
RefSeqiWP_013099327.1. NC_018079.1.
YP_006479903.1. NC_018079.1.

Genome annotation databases

EnsemblBacteriaiAFM62201; AFM62201; A3UG_22510.
GeneIDi13166366.
KEGGienl:A3UG_22510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003678 Genomic DNA. Translation: AFM62201.1 .
RefSeqi WP_013099327.1. NC_018079.1.
YP_006479903.1. NC_018079.1.

3D structure databases

ProteinModelPortali I6SPL1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFM62201 ; AFM62201 ; A3UG_22510 .
GeneIDi 13166366.
KEGGi enl:A3UG_22510.

Phylogenomic databases

KOi K00850.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Enterobacter cloacae subsp. dissolvens SDM, an efficient biomass-utilizing producer of platform chemical 2,3-butanediol."
    Xu Y., Wang A., Tao F., Su F., Tang H., Ma C., Xu P.
    J. Bacteriol. 194:897-898(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SDMImported.

Entry informationi

Entry nameiI6SPL1_ENTCL
AccessioniPrimary (citable) accession number: I6SPL1
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: September 3, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome

External Data

Dasty 3

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