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I6RGA8

- I6RGA8_PSEAI

UniProt

I6RGA8 - I6RGA8_PSEAI

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Protein
Elongation factor Tu
Gene
tuf, PADK2_03285, PADK2_03345
Organism
Pseudomonas aeruginosa DK2
Status
Unreviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTP By similarityUniRule annotation
Nucleotide bindingi81 – 855GTP By similarityUniRule annotation
Nucleotide bindingi136 – 1394GTP By similarityUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. GTPase activity Source: InterPro
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factorUniRule annotationImported

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPAER1093787:GLIP-665-MONOMER.
PAER1093787:GLIP-678-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
ORF Names:PADK2_03285Imported, PADK2_03345Imported
OrganismiPseudomonas aeruginosa DK2Imported
Taxonomic identifieri1093787 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002890: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK02358.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I6RGA8-1 [UniParc]FASTAAdd to Basket

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MAKEKFERNK PHVNVGTIGH VDHGKTTLTA ALTKVCSDTW GGSARAFDQI    50
DNAPEEKARG ITINTSHVEY DSAVRHYAHV DCPGHADYVK NMITGAAQMD 100
GAILVCSAAD GPMPQTREHI LLSRQVGVPY IVVFLNKADM VDDAELLELV 150
EMEVRDLLNT YDFPGDDTPI IIGSALMALE GKDDNGIGVS AVQKLVETLD 200
SYIPEPVRAI DQPFLMPIED VFSISGRGTV VTGRVERGII KVQEEVEIVG 250
IKATTKTTCT GVEMFRKLLD EGRAGENVGI LLRGTKREDV ERGQVLAKPG 300
TIKPHTKFEC EVYVLSKEEG GRHTPFFKGY RPQFYFRTTD VTGNCELPEG 350
VEMVMPGDNI KMVVTLIAPI AMEDGLRFAI REGGRTVGAG VVAKIIE 397
Length:397
Mass (Da):43,370
Last modified:October 3, 2012 - v1
Checksum:iA019D5BF8EBAB942
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003149 Genomic DNA. Translation: AFM62950.1.
CP003149 Genomic DNA. Translation: AFM62962.1.
RefSeqiYP_006480652.1. NC_018080.1.
YP_006480664.1. NC_018080.1.

Genome annotation databases

EnsemblBacteriaiAFM62950; AFM62950; PADK2_03285.
AFM62962; AFM62962; PADK2_03345.
GeneIDi13171120.
13171133.
KEGGipdk:PADK2_03285.
pdk:PADK2_03345.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003149 Genomic DNA. Translation: AFM62950.1 .
CP003149 Genomic DNA. Translation: AFM62962.1 .
RefSeqi YP_006480652.1. NC_018080.1.
YP_006480664.1. NC_018080.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFM62950 ; AFM62950 ; PADK2_03285 .
AFM62962 ; AFM62962 ; PADK2_03345 .
GeneIDi 13171120.
13171133.
KEGGi pdk:PADK2_03285.
pdk:PADK2_03345.

Phylogenomic databases

KOi K02358.

Enzyme and pathway databases

BioCyci PAER1093787:GLIP-665-MONOMER.
PAER1093787:GLIP-678-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_00118_B. EF_Tu_B.
InterProi IPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS00301. EFACTOR_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Deletion and acquisition of genomic content during early stage adaptation of Pseudomonas aeruginosa to a human host environment."
    Rau M.H., Marvig R.L., Ehrlich G.D., Molin S., Jelsbak L.
    Environ. Microbiol. 14:2200-2211(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DK2Imported.

Entry informationi

Entry nameiI6RGA8_PSEAI
AccessioniPrimary (citable) accession number: I6RGA8
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2012
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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