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I4EWF2 (I4EWF2_MODMB) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 7. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable DNA ligase HAMAP-Rule MF_00407
EC=6.5.1.1 HAMAP-Rule MF_00407
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:ligB EMBL CCH87715.1
Synonyms:lig HAMAP-Rule MF_00407
Ordered Locus Names:MODMU_2283 EMBL CCH87715.1
OrganismModestobacter marinus (strain BC501) [Complete proteome] [HAMAP]
Taxonomic identifier1144889 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Geodermatophilaceae › Modestobacter ›

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). RuleBase RU000617 HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. RuleBase RU004196 HAMAP-Rule MF_00407

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2121N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2101ATP By similarity HAMAP-Rule MF_00407
Binding site2171ATP By similarity HAMAP-Rule MF_00407
Binding site2321ATP By similarity HAMAP-Rule MF_00407
Binding site2611ATP By similarity HAMAP-Rule MF_00407
Binding site2971ATP By similarity HAMAP-Rule MF_00407
Binding site3731ATP By similarity HAMAP-Rule MF_00407
Binding site3791ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
I4EWF2 [UniParc].

Last modified September 5, 2012. Version 1.
Checksum: 781BCECD7E5E82B3

FASTA51053,622
        10         20         30         40         50         60 
MLLADVLAVS AAVSATRSRT AKAQAIADLV RRAGDAEVEP VTAWLSGELR QGRLGLGWRT 

        70         80         90        100        110        120 
LSRLTGEPAA EPSLTVGALD AALDELATTS GAGSAARRDA LVGAVLAAAT ESEQQFLVRL 

       130        140        150        160        170        180 
LTGELRQGAL EGVVLDAVAL AADVPAAAVR RAFMLSGRLP ETAATALTGG VAALDAVQLQ 

       190        200        210        220        230        240 
VGRPVRPMLA SPGSSLDAAL TDLGSEVTVE FKLDGARIQV HRDGDVVRVW TRTLREVTDG 

       250        260        270        280        290        300 
VPELVDRIRA LPCETAVLDG ETLALDDDGR PRPFQDTMSR FGSDSADAGV LLSPFLFDVL 

       310        320        330        340        350        360 
HLDGRDLLDE PLAVRLDALA GLLADDAHAA LRMPGVRRPT AEQAAGVLDD ALTAGHEGVV 

       370        380        390        400        410        420 
VKALDAPYAA GRRGKAWQKV KPVHTLDLVV LGAEWGYGRR SGKLSNIHLG ARDPDGGEPV 

       430        440        450        460        470        480 
MVGKTFKGMT DELLDWQTAT FPDLAAEQPP WGVLLRPELV VEIALDGAQR SPRYPGGVAL 

       490        500        510 
RFARVLRYRP DKTAAEADTI DAVRALLAGG

« Hide

References

[1]"Genome sequence of radiation-resistant Modestobacter marinus strain BC501, a representative Actinobacterium that thrives on calcareous stone surfaces."
Normand P., Gury J., Pujic P., Chouaia B., Crotti E., Brusetti L., Daffonchio D., Vacherie B., Barbe V., Medigue C., Calteau A., Ghodhbane-Gtari F., Essoussi I., Nouioui I., Abbassi-Ghozzi I., Gtari M.
J. Bacteriol. 194:4773-4774(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BC501 EMBL CCH87715.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FO203431 Genomic DNA. Translation: CCH87715.1.
RefSeqYP_006366202.1. NC_017955.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCCH87715; CCH87715; MODMU_2283.
GeneID13011040.
KEGGmmar:MODMU_2283.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01971.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 2 hits.
[Graphical view]
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF117018. SSF117018. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI4EWF2_MODMB
AccessionPrimary (citable) accession number: I4EWF2
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2012
Last sequence update: September 5, 2012
Last modified: April 3, 2013
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info