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I4D7N6 (I4D7N6_DESAJ) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Desaci_2899 EMBL AFM41810.1
OrganismDesulfosporosinus acidiphilus (strain DSM 22704 / JCM 16185 / SJ4) [Complete proteome] [HAMAP] EMBL AFM41810.1
Taxonomic identifier646529 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfosporosinus

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain145 – 350206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding178 – 23356ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3041Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3171Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3171Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3191Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I4D7N6 [UniParc].

Last modified September 5, 2012. Version 1.
Checksum: 9FBCFB4CEB7C2D22

FASTA36740,855
        10         20         30         40         50         60 
MENHKIRVIL LFGGRSGEHE VSLNSAQSIF KAIDRSRFDV ETIGINKQGQ WFWGLLPQTL 

        70         80         90        100        110        120 
LKEGFPETGE APQVTLVVDP SNPRFIALDG RPLPNDGKFD LIFPVLHGPY GEDGTLQGLL 

       130        140        150        160        170        180 
EMANVPYAGS GVLGSSLGMD KDRMKAAFLD KKLPLARFVT LLRSDYFNHP DNWVADIEAK 

       190        200        210        220        230        240 
IGYPCFVKPA NLGSSVGISK AHDRSELIQA LKLAADYDRK IVVEENIVGR EIEVAVLGND 

       250        260        270        280        290        300 
QPKASLPGEI LPAEEFYSYE AKYSDIGSSL KIPAPLPQFV IDQLQKMARE AFLAVDASGL 

       310        320        330        340        350        360 
SRVDFFVTEK NEIVLNEINT LPGFTEISMY PKLWEATGIP YSKLIDHLID LGFERYQEKQ 


KSRISRD 

« Hide

References

[1]"Complete Genome Sequences of Desulfosporosinus orientis DSM765T, Desulfosporosinus youngiae DSM17734T, Desulfosporosinus meridiei DSM13257T, and Desulfosporosinus acidiphilus DSM22704T."
Pester M., Brambilla E., Alazard D., Rattei T., Weinmaier T., Han J., Lucas S., Lapidus A., Cheng J.F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Teshima H., Detter J.C., Han C.S., Tapia R., Land M.L. expand/collapse author list , Hauser L., Kyrpides N.C., Ivanova N.N., Pagani I., Huntmann M., Wei C.L., Davenport K.W., Daligault H., Chain P.S., Chen A., Mavromatis K., Markowitz V., Szeto E., Mikhailova N., Pati A., Wagner M., Woyke T., Ollivier B., Klenk H.P., Spring S., Loy A.
J. Bacteriol. 194:6300-6301(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 22704 / JCM 16185 / SJ4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003639 Genomic DNA. Translation: AFM41810.1.
RefSeqYP_006467142.1. NC_018068.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFM41810; AFM41810; Desaci_2899.
GeneID13149965.
KEGGdai:Desaci_2899.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycDACI646529:GLCJ-2892-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI4D7N6_DESAJ
AccessionPrimary (citable) accession number: I4D7N6
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2012
Last sequence update: September 5, 2012
Last modified: February 19, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)