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I4CWN2 (I4CWN2_PSEST) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AFM34489.1
ORF Names:A458_16320 EMBL AFM34489.1
OrganismPseudomonas stutzeri CCUG 29243 [Complete proteome] EMBL AFM34489.1
Taxonomic identifier1196835 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain112 – 307196ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding138 – 19356ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2611Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2741Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2741Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2761Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I4CWN2 [UniParc].

Last modified September 5, 2012. Version 1.
Checksum: EAAC548858D2BFA7

FASTA31433,388
        10         20         30         40         50         60 
MTTLHSTRDP KAFGRVAVLF GGKSAEREVS LKSGAAVLAA LQEAGVDAFG IDAGDDLLQR 

        70         80         90        100        110        120 
LSNERIDRAF IVLHGRGGED GSMQGLLECA GIPYTGSGIL ASALAMDKLR TKQVWQSLGL 

       130        140        150        160        170        180 
PTPQHAVLAT EADCKAAAET LGFPLIVKPA HEGSSIGMAK VGGLTELIEA WRAASSYDSQ 

       190        200        210        220        230        240 
VLVEQWIQGP EFTVAVLHGQ VLPPIGLGTP HTFYDYDAKY LASDTQYRIP CGLDAVREQE 

       250        260        270        280        290        300 
LKTLSARACE AVGIRGWARV DVMQDGNGDF WLLEVNTVPG MTDHSLVPMA ARAAGLDFQQ 

       310 
LVLSILDDSL EAGS 

« Hide

References

[1]"Complete Genome Sequence of the Naphthalene-Degrading Bacterium Pseudomonas stutzeri AN10 (CCUG 29243)."
Brunet-Galmes I., Busquets A., Pena A., Gomila M., Nogales B., Garcia-Valdes E., Lalucat J., Bennasar A., Bosch R.
J. Bacteriol. 194:6642-6643(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: CCUG 29243 EMBL AFM34489.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003677 Genomic DNA. Translation: AFM34489.1.
RefSeqYP_006458912.1. NC_018028.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFM34489; AFM34489; A458_16320.
GeneID13145913.
KEGGpsc:A458_16320.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycPSTU1196835:GLIX-3283-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI4CWN2_PSEST
AccessionPrimary (citable) accession number: I4CWN2
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2012
Last sequence update: September 5, 2012
Last modified: June 11, 2014
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)