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Protein

Probable DNA ligase

Gene

lig

Organism
Mycobacterium chubuense (strain NBB4)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei213 – 2131ATPUniRule annotation
Active sitei215 – 2151N6-AMP-lysine intermediateUniRule annotation
Binding sitei220 – 2201ATPUniRule annotation
Binding sitei235 – 2351ATPUniRule annotation
Binding sitei264 – 2641ATPUniRule annotation
Binding sitei303 – 3031ATPUniRule annotation
Binding sitei375 – 3751ATPUniRule annotation
Binding sitei381 – 3811ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMCHU710421:GLGL-1763-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:Mycch_1764Imported
OrganismiMycobacterium chubuense (strain NBB4)Imported
Taxonomic identifieri710421 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000006057 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 PotentialImportedAdd
BLAST
Chaini28 – 514487 PotentialImportedPRO_5000912910Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Keywords - Domaini

SignalImported

Phylogenomic databases

KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 2 hits.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

I4BGZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLVEVAQAS ADVAAASARL AKIARIADLL RSAAAAGDPR LVAVVVSWLS
60 70 80 90 100
GELTQRQIGV GWASLRALPE PAAEPTLTVL GVDGALGDIG TTSGKGSQAR
110 120 130 140 150
RARLVAELFS AATAIEQTFL RRLLTGELRQ GALVGVMADA VSKAAGIDPA
160 170 180 190 200
AARRAAMLSG DLPTVAAAAV TGGVAALEAF TLQVGRPVGP MLAQTATSVA
210 220 230 240 250
DALERLDGRA VFEAKLDGAR VQIHRRGDSV SVFTRSLDDV TARLPEVVDA
260 270 280 290 300
ALALPVTDLI ADAEAIALRP DGRPQRFQVT ASRFGRRGGT AGPDTQKLSV
310 320 330 340 350
FFFDLLHVDG VDVLDQPAHE RVARLDAIVP DRQRVDRLLT TDPAEAQTFL
360 370 380 390 400
DTTLAAGHEG VMAKSPTAPY EAGRRGAGWL KVKPVHTLDL VVLAVEWGSG
410 420 430 440 450
RRTGKLSNIH LGARDPQTGG FVMLGKTFKG MTDEMLAWQT ERFLELADGL
460 470 480 490 500
TSGYSVRVRP EQVVEIAFDG LQTSSRYPGG MALRFARVLR YRDDKSAAEA
510
DTIDTVRALY ERSD
Length:514
Mass (Da):54,463
Last modified:September 5, 2012 - v1
Checksum:i593838EFAEA08301
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003053 Genomic DNA. Translation: AFM16555.1.
RefSeqiWP_014815036.1. NC_018027.1.
YP_006452234.1. NC_018027.1.

Genome annotation databases

EnsemblBacteriaiAFM16555; AFM16555; Mycch_1764.
KEGGimcb:Mycch_1764.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003053 Genomic DNA. Translation: AFM16555.1.
RefSeqiWP_014815036.1. NC_018027.1.
YP_006452234.1. NC_018027.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFM16555; AFM16555; Mycch_1764.
KEGGimcb:Mycch_1764.

Phylogenomic databases

KOiK10747.

Enzyme and pathway databases

BioCyciMCHU710421:GLGL-1763-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 2 hits.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Mycobacterium chubuense NBB4."
    US DOE Joint Genome Institute
    Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Mikhailova N., Teshima H., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Mattes T.
    , Holmes A., Rutledge P., Paulsen I., Coleman N., Woyke T.
    Submitted (JUN-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBB4Imported.

Entry informationi

Entry nameiI4BGZ8_MYCCN
AccessioniPrimary (citable) accession number: I4BGZ8
Entry historyi
Integrated into UniProtKB/TrEMBL: September 5, 2012
Last sequence update: September 5, 2012
Last modified: May 27, 2015
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.