lig

Functionⁱ

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityⁱ

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation

Cofactorⁱ

Mg²⁺UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	213	ATPUniRule annotation	1
Active siteⁱ	215	N6-AMP-lysine intermediateUniRule annotation	1
Binding siteⁱ	220	ATPUniRule annotation	1
Binding siteⁱ	235	ATPUniRule annotation	1
Binding siteⁱ	264	ATPUniRule annotation	1
Binding siteⁱ	303	ATPUniRule annotation	1
Binding siteⁱ	375	ATPUniRule annotation	1
Binding siteⁱ	381	ATPUniRule annotation	1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-UniRule
DNA binding Source: InterPro
DNA ligase (ATP) activity Source: UniProtKB-UniRule
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
cell division Source: UniProtKB-KW
DNA biosynthetic process Source: InterPro
DNA ligation involved in DNA repair Source: InterPro
DNA recombination Source: UniProtKB-UniRule
DNA replication Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	LigaseUniRule annotationImported
Biological process	Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation
Ligand	ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Probable DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation) Alternative name(s): Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesⁱ	Name:ligUniRule annotation Ordered Locus Names:Mycch_1764Imported
Organismⁱ	Mycobacterium chubuense (strain NBB4)Imported
Taxonomic identifierⁱ	710421 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Actinobacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium chubuense
Proteomesⁱ	UP000006057 Componentⁱ: Chromosome

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	291 – 415	DNA_LIGASE_A3InterPro annotationAdd BLAST		125

Sequence similaritiesⁱ

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

KEGG Orthology (KO) More...KOⁱ	K10747.
OMAⁱ	WLFEESY.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H0BGA.

Family and domain databases

Gene3Dⁱ	1.10.3260.10. 1 hit.
HAMAPⁱ	MF_00407. DNA_ligase. 1 hit.
InterProⁱ	View protein in InterPro IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR036599. DNA_ligase_N_sf. IPR012340. NA-bd_OB-fold.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit.
SUPFAMⁱ	SSF117018. SSF117018. 1 hit. SSF50249. SSF50249. 1 hit.
TIGRFAMsⁱ	TIGR00574. dnl1. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

I4BGZ8-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLLVEVAQAS ADVAAASARL AKIARIADLL RSAAAAGDPR LVAVVVSWLS 
        60         70         80         90        100
GELTQRQIGV GWASLRALPE PAAEPTLTVL GVDGALGDIG TTSGKGSQAR 
       110        120        130        140        150
RARLVAELFS AATAIEQTFL RRLLTGELRQ GALVGVMADA VSKAAGIDPA 
       160        170        180        190        200
AARRAAMLSG DLPTVAAAAV TGGVAALEAF TLQVGRPVGP MLAQTATSVA 
       210        220        230        240        250
DALERLDGRA VFEAKLDGAR VQIHRRGDSV SVFTRSLDDV TARLPEVVDA 
       260        270        280        290        300
ALALPVTDLI ADAEAIALRP DGRPQRFQVT ASRFGRRGGT AGPDTQKLSV 
       310        320        330        340        350
FFFDLLHVDG VDVLDQPAHE RVARLDAIVP DRQRVDRLLT TDPAEAQTFL 
       360        370        380        390        400
DTTLAAGHEG VMAKSPTAPY EAGRRGAGWL KVKPVHTLDL VVLAVEWGSG 
       410        420        430        440        450
RRTGKLSNIH LGARDPQTGG FVMLGKTFKG MTDEMLAWQT ERFLELADGL 
       460        470        480        490        500
TSGYSVRVRP EQVVEIAFDG LQTSSRYPGG MALRFARVLR YRDDKSAAEA 
       510 
DTIDTVRALY ERSD

Length:514

Mass (Da):54,463

Last modified:September 5, 2012 - v1

Checksum:ⁱ593838EFAEA08301

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP003053 Genomic DNA. Translation: AFM16555.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_014815036.1. NC_018027.1.

Genome annotation databases

EnsemblBacteriaⁱ	AFM16555; AFM16555; Mycch_1764.
KEGGⁱ	mcb:Mycch_1764.
PATRICⁱ	fig\|710421.3.peg.1767.

Similar proteinsⁱ

50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
I4BGZ8	P9WNV5 A5U776 I6YB16 A0A2C5SMN0 UPI0004F29523 UPI0006473FD4 UPI0004D681BF UPI0006DC92D0 UPI0004941AF0 UPI0002A5A4DF +1055	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) Mycobacterium tuberculosis Mycobacterium bovis Mycobacterium canettii Mycobacterium africanum (strain GM041182) Mycobacterium canettii (strain CIPT 140010059) Mycobacterium africanum Mycobacterium tuberculosis complex Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) And more	507	UniRef50_P9WNV5	Cluster: DNA ligase B	1,066

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP003053 Genomic DNA. Translation: AFM16555.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_014815036.1. NC_018027.1.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AFM16555; AFM16555; Mycch_1764.
KEGGⁱ	mcb:Mycch_1764.
PATRICⁱ	fig\|710421.3.peg.1767.

Phylogenomic databases

KEGG Orthology (KO) More...KOⁱ	K10747.
OMAⁱ	WLFEESY.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H0BGA.

Family and domain databases

Gene3Dⁱ	1.10.3260.10. 1 hit.
HAMAPⁱ	MF_00407. DNA_ligase. 1 hit.
InterProⁱ	View protein in InterPro IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR036599. DNA_ligase_N_sf. IPR012340. NA-bd_OB-fold.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit.
SUPFAMⁱ	SSF117018. SSF117018. 1 hit. SSF50249. SSF50249. 1 hit.
TIGRFAMsⁱ	TIGR00574. dnl1. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	I4BGZ8_MYCCN
Accessionⁱ	I4BGZ8Primary (citable) accession number: I4BGZ8
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	September 5, 2012
	Last sequence update:	September 5, 2012
	Last modified:	March 28, 2018
	This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteomeImported

UniProtKB - I4BGZ8 (I4BGZ8_MYCCN)

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Probable DNA ligase

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Functionⁱ

Catalytic activityⁱ

Cofactorⁱ

Sites

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Family & Domainsⁱ

Domains and Repeats

Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

Sequenceⁱ

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - I4BGZ8 (I4BGZ8_MYCCN)

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Probable DNA ligase

lig

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

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Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

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Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ