lig

Functionⁱ

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityⁱ

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactorⁱ

Mg²⁺UniRule annotation

Sites

Feature key	Position(s)	Length	Description
Binding siteⁱ	213 – 213	1	ATPUniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407
Active siteⁱ	215 – 215	1	N6-AMP-lysine intermediateUniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407
Binding siteⁱ	220 – 220	1	ATPUniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407
Binding siteⁱ	235 – 235	1	ATPUniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407
Binding siteⁱ	264 – 264	1	ATPUniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407
Binding siteⁱ	303 – 303	1	ATPUniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407
Binding siteⁱ	375 – 375	1	ATPUniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407
Binding siteⁱ	381 – 381	1	ATPUniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-HAMAP
DNA binding Source: InterPro
DNA ligase (ATP) activity Source: UniProtKB-HAMAP
metal ion binding Source: UniProtKB-KW

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
cell division Source: UniProtKB-KW
DNA biosynthetic process Source: InterPro
DNA ligation involved in DNA repair Source: InterPro
DNA recombination Source: UniProtKB-HAMAP
DNA replication Source: UniProtKB-HAMAP

Complete GO annotation...

Keywords - Molecular functionⁱ

LigaseUniRule annotation

Keywords - Biological processⁱ

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandⁱ

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	MCHU710421:GLGL-1763-MONOMER.

BioCycⁱ

MCHU710421:GLGL-1763-MONOMER.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Probable DNA ligaseUniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407 (EC:6.5.1.1UniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407 ) Alternative name(s): Polydeoxyribonucleotide synthase [ATP]UniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407
Gene namesⁱ	Name:ligUniRule annotation Automatic assertion according to rulesⁱ HAMAP-Rule:MF_00407 Ordered Locus Names:Mycch_1764Imported Automatic assertion inferred from database entriesⁱ EMBL:AFM16555.1
Organismⁱ	Mycobacterium chubuense (strain NBB4)Imported Automatic assertion inferred from database entriesⁱ EMBL:AFM16555.1 Proteomes:UP000006057
Taxonomic identifierⁱ	710421 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium chubuense
Proteomesⁱ	UP000006057: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Signal peptideⁱ	1 – 27	27	PotentialImported Automatic assertion inferred from database entriesⁱ EMBL:AFM16555.1			Add BLAST
Chainⁱ	28 – 514	487	PotentialImported Automatic assertion inferred from database entriesⁱ EMBL:AFM16555.1		PRO_5000912910	Add BLAST

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Keywords - Domainⁱ

SignalImported

Phylogenomic databases

KOⁱ	K01971.

KOⁱ

K01971.

Family and domain databases

Gene3Dⁱ	1.10.3260.10. 1 hit. 2.40.50.140. 2 hits.
HAMAPⁱ	MF_00407. DNA_ligase.
InterProⁱ	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfamⁱ	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAMⁱ	SSF117018. SSF117018. 1 hit. SSF50249. SSF50249. 1 hit.
TIGRFAMsⁱ	TIGR00574. dnl1. 1 hit.
PROSITEⁱ	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

I4BGZ8-1 [UniParc]FASTA Add to Basket

« Hide

        10         20         30         40         50
MLLVEVAQAS ADVAAASARL AKIARIADLL RSAAAAGDPR LVAVVVSWLS 
        60         70         80         90        100
GELTQRQIGV GWASLRALPE PAAEPTLTVL GVDGALGDIG TTSGKGSQAR 
       110        120        130        140        150
RARLVAELFS AATAIEQTFL RRLLTGELRQ GALVGVMADA VSKAAGIDPA 
       160        170        180        190        200
AARRAAMLSG DLPTVAAAAV TGGVAALEAF TLQVGRPVGP MLAQTATSVA 
       210        220        230        240        250
DALERLDGRA VFEAKLDGAR VQIHRRGDSV SVFTRSLDDV TARLPEVVDA 
       260        270        280        290        300
ALALPVTDLI ADAEAIALRP DGRPQRFQVT ASRFGRRGGT AGPDTQKLSV 
       310        320        330        340        350
FFFDLLHVDG VDVLDQPAHE RVARLDAIVP DRQRVDRLLT TDPAEAQTFL 
       360        370        380        390        400
DTTLAAGHEG VMAKSPTAPY EAGRRGAGWL KVKPVHTLDL VVLAVEWGSG 
       410        420        430        440        450
RRTGKLSNIH LGARDPQTGG FVMLGKTFKG MTDEMLAWQT ERFLELADGL 
       460        470        480        490        500
TSGYSVRVRP EQVVEIAFDG LQTSSRYPGG MALRFARVLR YRDDKSAAEA 
       510 
DTIDTVRALY ERSD

Length:514

Mass (Da):54,463

Last modified:September 5, 2012 - v1

Checksum:ⁱ593838EFAEA08301

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP003053 Genomic DNA. Translation: AFM16555.1.
RefSeqⁱ	YP_006452234.1. NC_018027.1.

Genome annotation databases

EnsemblBacteriaⁱ	AFM16555; AFM16555; Mycch_1764.
GeneIDⁱ	13144574.
KEGGⁱ	mcb:Mycch_1764.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP003053 Genomic DNA. Translation: AFM16555.1.
RefSeqⁱ	YP_006452234.1. NC_018027.1.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AFM16555; AFM16555; Mycch_1764.
GeneIDⁱ	13144574.
KEGGⁱ	mcb:Mycch_1764.

Phylogenomic databases

KOⁱ	K01971.

KOⁱ

K01971.

Enzyme and pathway databases

BioCycⁱ	MCHU710421:GLGL-1763-MONOMER.

BioCycⁱ

MCHU710421:GLGL-1763-MONOMER.

Family and domain databases

Gene3Dⁱ	1.10.3260.10. 1 hit. 2.40.50.140. 2 hits.
HAMAPⁱ	MF_00407. DNA_ligase.
InterProⁱ	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfamⁱ	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAMⁱ	SSF117018. SSF117018. 1 hit. SSF50249. SSF50249. 1 hit.
TIGRFAMsⁱ	TIGR00574. dnl1. 1 hit.
PROSITEⁱ	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNetⁱ	Search...

Publicationsⁱ

"Complete sequence of chromosome of Mycobacterium chubuense NBB4."
US DOE Joint Genome Institute
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Mikhailova N., Teshima H., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Mattes T.
Woyke T.
Submitted (JUN-2012) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain:
NBB4
Imported
Automatic assertion inferred from database entriesⁱ
- EMBL:AFM16555.1
- Proteomes:UP000006057
.

Entry informationⁱ

Entry nameⁱ

I4BGZ8_MYCCN

Accessionⁱ

Primary (citable) accession number: I4BGZ8

Entry historyⁱ

Integrated into UniProtKB/TrEMBL:	September 5, 2012
Last sequence update:	September 5, 2012
Last modified:	February 4, 2015
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry statusⁱ

Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

Proteomes

UniRef

Supporting data

UniParc

Help

I4BGZ8 - I4BGZ8_MYCCN

UniProt

I4BGZ8 - I4BGZ8_MYCCN

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Probable DNA ligase

Select a section on the left to see content.

<p>Provides any useful information about the protein, mostly biological knowledge.</p><p><a href='../manual/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>Provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.</p><p><a href='../manual/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>Describes post-translational modifications (PTMs) and/or processing events.</p><p><a href='../manual/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>Provides information on sequence similarities with other proteins and the domain(s) present in a protein.</p><p><a href='../manual/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>Provides information about the sequence similarity with other proteins.</p><p><a href='../manual/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>Displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.</p><p><a href='../manual/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>Is used to point to information related to entries and found in data collections other than UniProtKB.</p><p><a href='../manual/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>Contains the literature citations that are the sources of data used to annotate the entry. Each reference is numbered and contains several subsections allowing a precise description of a given citation.</p><p><a href='../manual/publications_section' target='_top'>More...</a></p>Publicationsi

<p>Provides general information on the entry.</p><p><a href='../manual/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>Contains any relevant information that doesn’t fit in any other defined sections</p><p><a href='../manual/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

External Data

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Publicationsⁱ

Entry informationⁱ

Miscellaneousⁱ