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Protein

D-alanine--D-alanine ligase

Gene

Fleli_0247

Organism
Bernardetia litoralis (strain ATCC 23117 / DSM 6794 / NBRC 15988 / NCIMB 1366 / Sio-4) (Flexibacter litoralis)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.SAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.SAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+SAAS annotation
  • Mn2+SAAS annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.SAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseSAAS annotationImported
Biological processCell shape, Cell wall biogenesis/degradationSAAS annotation, Peptidoglycan synthesisSAAS annotation
LigandATP-bindingPROSITE-ProRule annotationSAAS annotation, MagnesiumSAAS annotation, ManganeseSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciFLIT880071:GLDC-230-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseSAAS annotation (EC:6.3.2.4SAAS annotation)
Gene namesi
Ordered Locus Names:Fleli_0247Imported
OrganismiBernardetia litoralis (strain ATCC 23117 / DSM 6794 / NBRC 15988 / NCIMB 1366 / Sio-4) (Flexibacter litoralis)Imported
Taxonomic identifieri880071 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesBernardetiaceaeBernardetia
Proteomesi
  • UP000006054 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm SAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini252 – 469ATP-graspInterPro annotationAdd BLAST218

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.SAAS annotation

Phylogenomic databases

KOiK01921.
OMAiVHTIPKD.
OrthoDBiPOG091H06GK.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
PfamiView protein in Pfam
PF07478. Dala_Dala_lig_C. 2 hits.
PF01820. Dala_Dala_lig_N. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.

Sequencei

Sequence statusi: Complete.

I4AFK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFVVLISTHN FFFQDNNSLL MIKIGVFFGG VSREREISFS GAKTVSQTLN
60 70 80 90 100
RSLFEPVYIL VDSLGKFIKI NPVQFEHKAI SHFYPTQESI PEEYDEFSPV
110 120 130 140 150
YIETLENLTK EKHREIMEEV GTPISTENLS EHIDFALLML HGTFAEDGML
160 170 180 190 200
QGLFEWLQIP YSGCGIFSSS FSIDKHLQQV VNEKLNLGDN RKYQMIRKEE
210 220 230 240 250
WQKCDKKEFF EDLKSKLGLP IVIKAPYQGS SIGVSILKND NLEEFIKAVN
260 270 280 290 300
TSLFARQISK DEWTQLTMPQ RKEYVNVLMS LEKGIGLPII FEEKSLLGGN
310 320 330 340 350
LGEQIIYSPL KLIEKLNDFF SYSEENATLF SFESEDMVLF EECLSGKEFS
360 370 380 390 400
CGVIQNENLE VVALPPTEII TTTKVYDFEA KYLPNASQKR IPIDIPTDKI
410 420 430 440 450
IEIQEKCKAI FQTFKFDACA RIDGFYTDDN RIEIIDINTL PGMSPTSLIF
460 470 480 490 500
RQAAEVGFSP TDFMTYLVYQ SLNCRVHTAK RPYFFRNLFK KLESELLDSA
510 520 530 540 550
YHKKEKVAIF FDETIFDEAR KEYNSIASEG KQNPFCVFVK TENNQEVTLY
560 570 580 590 600
KLPVSFLLKP NIEEVIQNLE TELHPAISHT IEKAKELTDF FVKDFVSVPQ
610 620 630 640
KITLENLNDM TNDAVWLLRS PNNEDAAQSK LKRQLYEIGM AVVD
Length:644
Mass (Da):74,069
Last modified:September 5, 2012 - v1
Checksum:iD848A063EED8FB0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003345 Genomic DNA. Translation: AFM02736.1.
RefSeqiWP_014796201.1. NC_018018.1.

Genome annotation databases

EnsemblBacteriaiAFM02736; AFM02736; Fleli_0247.
KEGGifli:Fleli_0247.
PATRICifig|880071.3.peg.236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003345 Genomic DNA. Translation: AFM02736.1.
RefSeqiWP_014796201.1. NC_018018.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFM02736; AFM02736; Fleli_0247.
KEGGifli:Fleli_0247.
PATRICifig|880071.3.peg.236.

Phylogenomic databases

KOiK01921.
OMAiVHTIPKD.
OrthoDBiPOG091H06GK.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciFLIT880071:GLDC-230-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
PfamiView protein in Pfam
PF07478. Dala_Dala_lig_C. 2 hits.
PF01820. Dala_Dala_lig_N. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiI4AFK1_BERLS
AccessioniPrimary (citable) accession number: I4AFK1
Entry historyiIntegrated into UniProtKB/TrEMBL: September 5, 2012
Last sequence update: September 5, 2012
Last modified: June 7, 2017
This is version 37 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.