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I4A995 (I4A995_DESDJ) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Desde_2184 EMBL AFM00530.1
OrganismDesulfitobacterium dehalogenans (strain ATCC 51507 / DSM 9161 / JW/IU-DC1) [Complete proteome] [HAMAP] EMBL AFM00530.1
Taxonomic identifier756499 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfitobacterium

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain140 – 345206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding173 – 22856ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2991Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3121Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3121Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3141Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I4A995 [UniParc].

Last modified September 5, 2012. Version 1.
Checksum: 8C34B465AF5F71C7

FASTA36140,016
        10         20         30         40         50         60 
MTRQKIIILF GGQSGEHEVS LNSASSVLKA IDKEKYEVET LGISKSGQWH WGIRPEQWQQ 

        70         80         90        100        110        120 
DPTLQGILVN LVHDPSNPRF IALDGSTLPQ DGTFDLIFPV LHGPFGEDGT IQGLFEMANI 

       130        140        150        160        170        180 
PYAGSGVLGS ALGMDKDRMK AVFTEAGLPQ APYLTVLRTT LQKMMEKVLH DIEHSLGYPC 

       190        200        210        220        230        240 
FVKPANLGSS VGISKARNRE ELINALHLAA EYDRKIVVEQ NIVGREIELS VLGNEDAKAS 

       250        260        270        280        290        300 
IPGEILPSKD FYDYEAKYID TGSRLVIPAP LDQEVVKSLQ EKAVAAFKAV EACGLSRVDF 

       310        320        330        340        350        360 
FVTEGNQIYI NEINTMPGFT QISMYPKLWE ASGIPYSELI TQLIELGFQR FRDSRSRKIS 


L 

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References

[1]"Complete sequence of Desulfitobacterium dehalogenans ATCC 51507."
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Teshima H., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Kruse T. expand/collapse author list , de Vos W.M., Smidt H., Woyke T.
Submitted (JUN-2012) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51507 / DSM 9161 / JW/IU-DC1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003348 Genomic DNA. Translation: AFM00530.1.
RefSeqYP_006430323.1. NC_018017.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFM00530; AFM00530; Desde_2184.
GeneID13117227.
KEGGddh:Desde_2184.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycDDEH756499:GLCG-2177-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI4A995_DESDJ
AccessionPrimary (citable) accession number: I4A995
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2012
Last sequence update: September 5, 2012
Last modified: June 11, 2014
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)