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Protein

DNA ligase

Gene

lig

Organism
Thermococcus cleftensis
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation
NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei247ATPUniRule annotation1
Active sitei249N6-AMP-lysine intermediateUniRule annotation1
Binding sitei254ATPUniRule annotation1
Binding sitei269ATPUniRule annotation1
Binding sitei299ATPUniRule annotation1
Binding sitei339ATPUniRule annotation1
Binding sitei414ATPUniRule annotation1
Binding sitei420ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.6UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP/NAD(+)]UniRule annotation
Gene namesi
Name:ligUniRule annotation
ORF Names:CL1_1197Imported
OrganismiThermococcus cleftensisImported
Taxonomic identifieri163003 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000006064 Componenti: Chromosome

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini327 – 455DNA_LIGASE_A3InterPro annotationAdd BLAST129

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I3ZUL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYSELTELY RRLEKTTLKT LKTRFVAEFL RKTPDDVLEV VPYLILGKVF
60 70 80 90 100
PDWDERELGV GEKLLIKSVS IATGVSEREI ENSVKDTGDL GESVALALKK
110 120 130 140 150
KKQRSFFSQP LTIKRVYDTF VKIAEAQGEG SQDRKMKYLA NLFMDAKPEE
160 170 180 190 200
GKYLARTVLG TMRTGVAEGI LRDAIAEAFK VKPELVERAY MLTSDFGHVA
210 220 230 240 250
KIAKLEGNEG LSRVRIQVGK PIRPMLAQNA ASVKEALAEM GGKAAFEIKY
260 270 280 290 300
DGARVQVHKD GDRVVIYSRR LENVTRSIPE IVRAVRESLK AEKAIVEGEL
310 320 330 340 350
VAVGEGGKPR PFQYVLRRFR RKYNIEEMIE KIPLELNLFD VLYVDGEGLI
360 370 380 390 400
DTPFSERREK LEEIISPGER IRLAEQLVTK SAEEAEAFYK KALELGHEGL
410 420 430 440 450
MAKRLDSVYE PGNRGKKWLK IKPTMEDLDL VIIGAEWGEG RRAHLLGSFL
460 470 480 490 500
VAAFDPHSGE FVPVGKVGSG FTDEDLAEFT KMLKPLIVRE EGKYVEIEPK
510 520 530 540 550
VVIQVTYQEI QRSPKYESGF ALRFPRYVAL REDKSPEEAD TIERIAQLYE

FQERFKAKR
Length:559
Mass (Da):63,488
Last modified:September 5, 2012 - v1
Checksum:i934B59576B22B39B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003651 Genomic DNA. Translation: AFL95400.1.
RefSeqiWP_014789033.1. NC_018015.1.

Genome annotation databases

EnsemblBacteriaiAFL95400; AFL95400; CL1_1197.
GeneIDi13037587.
KEGGithm:CL1_1197.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003651 Genomic DNA. Translation: AFL95400.1.
RefSeqiWP_014789033.1. NC_018015.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFL95400; AFL95400; CL1_1197.
GeneIDi13037587.
KEGGithm:CL1_1197.

Phylogenomic databases

KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiI3ZUL6_9EURY
AccessioniPrimary (citable) accession number: I3ZUL6
Entry historyi
Integrated into UniProtKB/TrEMBL: September 5, 2012
Last sequence update: September 5, 2012
Last modified: November 2, 2016
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.