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I3XAX9 (I3XAX9_RHIFR) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 8. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AFL53035.1
ORF Names:USDA257_c44970 EMBL AFL53035.1
OrganismSinorhizobium fredii USDA 257 EMBL AFL53035.1
Taxonomic identifier1185652 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium ›

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. SAAS SAAS005905 HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. SAAS SAAS005905 HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity SAAS SAAS005905 HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. SAAS SAAS005905 HAMAP-Rule MF_00047

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain102 – 302201ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding129 – 18355ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2521Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2691Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2691Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2711Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I3XAX9 [UniParc].

Last modified September 5, 2012. Version 1.
Checksum: 61B6FE17E0449FD2

FASTA30833,466
        10         20         30         40         50         60 
MSGRHLAVLM GGFSSERPVS LSSGAACADA LEAEGYRVSR VDVGRDVAAV LADLRPDVVF 

        70         80         90        100        110        120 
NALHGPFGED GTIQGILEYL EIPYTHSGVL ASALAMDKAQ AKHVAKAAGI PVADALIMDR 

       130        140        150        160        170        180 
REFGNVHPMR PPYVVKPVRE GSSFGVVIVK EDQSHPPQVI TSSEWRYGDR VMVERYIAGR 

       190        200        210        220        230        240 
ELTCGVMGDV ALGVTEIIPQ GHAFYDYDSK YVKGGSTHVI PARISPNIYQ KIQSLAVKAH 

       250        260        270        280        290        300 
QAIGCRGVSR SDFRFDDRGD GEGELIWLEI NTQPGMTPTS LVPEMAQHAG LQFGEFLRWM 


VEDASCLR

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References

[1]"Complete Genome Sequence of the Broad-Host-Range Strain Sinorhizobium fredii USDA257."
Schuldes J., Rodriguez Orbegoso M., Schmeisser C., Krishnan H.B., Daniel R., Streit W.R.
J. Bacteriol. 194:4483-4483(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: USDA 257 EMBL AFL53035.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003563 Genomic DNA. Translation: AFL53035.1.
RefSeqYP_006399792.1. NC_018000.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFL53035; AFL53035; USDA257_c44970.
GeneID13064792.
KEGGsfd:USDA257_c44970.

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI3XAX9_RHIFR
AccessionPrimary (citable) accession number: I3XAX9
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2012
Last sequence update: September 5, 2012
Last modified: April 3, 2013
This is version 8 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info