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I3V097 (I3V097_PSEPU) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
ORF Names:YSA_08133 EMBL AFK71168.1
OrganismPseudomonas putida ND6 [Complete proteome] EMBL AFK71168.1
Taxonomic identifier231023 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain51 – 254204ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding78 – 13356ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2081Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2211Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2211Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2231Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I3V097 [UniParc].

Last modified September 5, 2012. Version 1.
Checksum: 50718CCDD9E10ACF

FASTA26528,381
        10         20         30         40         50         60 
MEIGIDLLFP ALHGRWGEDG TIQGLAAMCG LPCVGSGVLG SAMAMDKDVT KRMVQSAGLV 

        70         80         90        100        110        120 
VGPWLALNSM RPWEELVECL GSSTLFVKPA TSGSSIGVSR VSNALEYAAA FAIAAREDTK 

       130        140        150        160        170        180 
VLVEAAVCGR EIECGVLELE EGLMASVVGE IIKKEGHAYY DYQAKYDSNS VTGLRVPSLL 

       190        200        210        220        230        240 
PPDIVARIQA LSVQAFRCLE LKGYARVDFF LTEEGEIILN EINTLPGFTS ASMYPKMFEC 

       250        260 
SGYPPPKLVG ALVEYGLSSA SKERL 

« Hide

References

[1]"Complete Genome Sequence of the Naphthalene-Degrading Pseudomonas putida Strain ND6."
Li S., Zhao H., Li Y., Niu S., Cai B.
J. Bacteriol. 194:5154-5155(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ND6 EMBL AFK71168.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003588 Genomic DNA. Translation: AFK71168.1.
RefSeqYP_006388283.1. NC_017986.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFK71168; AFK71168; YSA_08133.
GeneID12993400.
KEGGppi:YSA_08133.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycPPUT231023:GLIT-4469-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI3V097_PSEPU
AccessionPrimary (citable) accession number: I3V097
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2012
Last sequence update: September 5, 2012
Last modified: June 11, 2014
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)