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I3TVR0 (I3TVR0_TISMK) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:TMO_c0238 EMBL AFK56848.1
Encoded onPlasmid pTM3 Ref.1 EMBL AFK56848.1
OrganismTistrella mobilis (strain KA081020-065) [Complete proteome] [HAMAP] EMBL AFK56848.1
Taxonomic identifier1110502 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeTistrella

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain102 – 298197ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding128 – 18053ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2481Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2651Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2651Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2671Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I3TVR0 [UniParc].

Last modified September 5, 2012. Version 1.
Checksum: C6EE77892998FE53

FASTA30432,702
        10         20         30         40         50         60 
MTTQHVAVLM GGMSAEREVS LTSGRACAEA LRGEGYRVTE IDAGADLGAK LAEIRPDVVF 

        70         80         90        100        110        120 
NALHGRFGED GCVQGVMNLM RLPYTHSGLL ASAIAMDKPV AKRLYAAAGL PVAEDRVMHI 

       130        140        150        160        170        180 
DEIGREHPMA VPYVLKPAQE GSSVGVRIVR DESETPLGNR SWTHGPVVLV ERYIPGRELT 

       190        200        210        220        230        240 
VGVMDGRALG LTEIRPVQNF YDYEAKYTDG RAVHLVPAPV PEDLAVRAKE IAEAAHRALG 

       250        260        270        280        290        300 
CRGVSRADLR WDDTKPGTEG LILLEVNTQP GMTPLSLVPE QAAAVGIAFG PLVRWMVEHA 


ACDA 

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References

[1]"Bacterial biosynthesis and maturation of the didemnin anti-cancer agents."
Xu Y., Kersten R.D., Nam S.J., Lu L., Al-Suwailem A.M., Zheng H., Fenical W., Dorrestein P.C., Moore B.S., Qian P.Y.
J. Am. Chem. Soc. 134:8625-8632(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KA081020-065 EMBL AFK56848.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003239 Genomic DNA. Translation: AFK56848.1.
RefSeqYP_006373830.1. NC_017958.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFK56848; AFK56848; TMO_c0238.
GeneID13001565.
KEGGtmo:TMO_c0238.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.
OMALCRWIVE.

Enzyme and pathway databases

BioCycTMOB1110502:GLMJ-3851-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI3TVR0_TISMK
AccessionPrimary (citable) accession number: I3TVR0
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2012
Last sequence update: September 5, 2012
Last modified: June 11, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)