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I3TJN7 (I3TJN7_TISMK) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 7. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108
Gene names
Ordered Locus Names:TMO_1136 EMBL AFK52975.1
OrganismTistrella mobilis (strain KA081020-065) [Complete proteome] [HAMAP] EMBL AFK52975.1
Taxonomic identifier1110502 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Tistrella ›

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. RuleBase RU004443 PIRNR PIRNR000108

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding85 – 873NADP By similarity PIRSR PIRSR000108-4
Nucleotide binding319 – 3246NADP By similarity PIRSR PIRSR000108-4
Region104 – 1107Substrate binding By similarity PIRSR PIRSR000108-2

Sites

Metal binding2611Magnesium or manganese By similarity PIRSR PIRSR000108-3
Metal binding2841Magnesium or manganese By similarity PIRSR PIRSR000108-3
Binding site871Substrate By similarity PIRSR PIRSR000108-2
Binding site921NADP By similarity PIRSR PIRSR000108-4
Binding site1191Substrate By similarity PIRSR PIRSR000108-2
Binding site1421Substrate By similarity PIRSR PIRSR000108-2
Binding site2691NADP By similarity PIRSR PIRSR000108-4
Binding site3371NADP; via amide nitrogen and carbonyl oxygen By similarity PIRSR PIRSR000108-4
Site1491Critical for catalysis By similarity PIRSR PIRSR000108-1
Site2211Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

Sequence LengthMass (Da)Tools
I3TJN7 [UniParc].

Last modified September 5, 2012. Version 1.
Checksum: BA26676BD26AD356

FASTA41546,329
        10         20         30         40         50         60 
MPRIAEEIVR MTKIKVANPV VELDGDEMTR IIWRFIKDKL ILPYLDVDLL YYDLGVEHRD 

        70         80         90        100        110        120 
ATNDKVTVDA AEAIAKHGVG VKCATITPDE ARVEEFKLKQ MWKSPNGTIR NILGGTVFRE 

       130        140        150        160        170        180 
PIICRNVPRL VPGWTQPIVI GRHAFGDQYR ATDFKVPGKG TLTVRFQPED GGEAIEYEVF 

       190        200        210        220        230        240 
KFPGSGVAMA MYNLDESIRG FARACMNYGL QRGYPVYLST KNTILKAYDG RFKDLFQEVF 

       250        260        270        280        290        300 
DAEFADQFKA AGIVYEHRLI DDMVASALKW DGGFVWACKN YDGDVQSDTV AQGFGSLGLM 

       310        320        330        340        350        360 
TSVLMTPDGK TVEAEAAHGT VTRHFREHQK GRETSTNPIA SIFAWTRGLG YRGKFDGNDA 

       370        380        390        400        410 
LIGFAETLER VCVETVEAGH MTKDLAILVG KDQAWLNTNA FLDKIDENLK KAMAS

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References

[1]"Bacterial biosynthesis and maturation of the didemnin anti-cancer agents."
Xu Y., Kersten R.D., Nam S.J., Lu L., Al-Suwailem A.M., Zheng H., Fenical W., Dorrestein P.C., Moore B.S., Qian P.Y.
J. Am. Chem. Soc. 134:8625-8632(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KA081020-065 EMBL AFK52975.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003236 Genomic DNA. Translation: AFK52975.1.
RefSeqYP_006370559.1. NC_017956.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFK52975; AFK52975; TMO_1136.
GeneID13004247.
KEGGtmo:TMO_1136.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00031.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI3TJN7_TISMK
AccessionPrimary (citable) accession number: I3TJN7
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2012
Last sequence update: September 5, 2012
Last modified: April 3, 2013
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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