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Protein

Propionyl-CoA carboxylase, carboxyltransferase subunit

Gene

pccB

Organism
Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the propionyl coenzyme A carboxylase (PCC) complex involved in propionate utilization and in the production of the poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV), which is a water-insoluble biopolymer used as intracellular energy reserve material when cells grow under conditions of nutrient limitation. The complex catalyzes the carboxylation of propionyl-CoA to methylmalonyl-CoA. PCC is also able to catalyze the carboxylation of acetyl-CoA.1 Publication

Catalytic activityi

ATP + propanoyl-CoA + HCO3- = ADP + phosphate + (S)-methylmalonyl-CoA.1 Publication

Pathwayi: propanoyl-CoA degradation

This protein is involved in step 1 of the subpathway that synthesizes succinyl-CoA from propanoyl-CoA.1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Propionyl-CoA carboxylase, carboxyltransferase subunit (pccB), Propionyl-CoA carboxylase, biotin carboxylase and biotin-carboxyl carrier subunit (pccA), Propionyl-CoA carboxylase, protein PccX subunit (pccX)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway propanoyl-CoA degradation, which is itself part of Metabolic intermediate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinyl-CoA from propanoyl-CoA, the pathway propanoyl-CoA degradation and in Metabolic intermediate metabolism.

GO - Molecular functioni

  • lyase activity Source: UniProtKB-KW
  • propionyl-CoA carboxylase activity Source: UniProtKB

Keywordsi

Molecular functionLigase, Lyase

Enzyme and pathway databases

UniPathwayiUPA00945; UER00908.

Names & Taxonomyi

Protein namesi
Recommended name:
Propionyl-CoA carboxylase, carboxyltransferase subunit1 Publication (EC:6.4.1.31 Publication)
Short name:
PCC1 Publication
Gene namesi
Name:pccB1 Publication
Synonyms:accA
Ordered Locus Names:HFX_2478Imported
OrganismiHaloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei)
Taxonomic identifieri523841 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHaloferax
Proteomesi
  • UP000006469 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking pccB and pccX genes accumulate a high level of propionyl-CoA, which would then inhibit CoA-dependent enzymes such as succinyl-CoA synthetase and could partially inhibit the TCA cycle. Also affected in growth and glucose utilization. Cells are unable to produce PHBV and show morphological abnormalitiess such as a decrease of PHBV granules.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004396361 – 516Propionyl-CoA carboxylase, carboxyltransferase subunitAdd BLAST516

Interactioni

Subunit structurei

The propionyl coenzyme A carboxylase (PCC) complex is composed of three subunits: PccA (biotin carboxylase and biotin-carboxyl carrier), PccB (carboxyltransferase) and PccX.1 Publication

Structurei

3D structure databases

SMRiI3R7F1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 259CoA carboxyltransferase N-terminalPROSITE-ProRule annotationAdd BLAST257
Domaini263 – 509CoA carboxyltransferase C-terminalPROSITE-ProRule annotationAdd BLAST247

Sequence similaritiesi

Belongs to the AccD/PCCB family.Curated

Phylogenomic databases

KOiK19312.
OMAiPIIFMED.
OrthoDBiPOG093Z02GE.

Family and domain databases

InterProiView protein in InterPro
IPR034733. AcCoA_carboxyl.
IPR029045. ClpP/crotonase-like_dom.
IPR011763. COA_CT_C.
IPR011762. COA_CT_N.
PfamiView protein in Pfam
PF01039. Carboxyl_trans. 1 hit.
SUPFAMiSSF52096. SSF52096. 2 hits.
PROSITEiView protein in PROSITE
PS50989. COA_CT_CTER. 1 hit.
PS50980. COA_CT_NTER. 1 hit.

Sequencei

Sequence statusi: Complete.

I3R7F1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMEDRIDEL REKREEALKG GGEDRIASQH DKGKMTARER IDYFLDDGTF
60 70 80 90 100
REFDQFRTHR NHKFGMEETK LPGDGVITGH GEVDGRTVFV FAHDFTVFGG
110 120 130 140 150
SLGEVFAEKI CKVMDKAMEV GAPVIGLNDS AGARIQEGVQ SLGGFGEIFR
160 170 180 190 200
RNTEASGVVP QISAIMGPCA GGAVYSPALT DFTFMVRDTS HMFITGPDVI
210 220 230 240 250
KTVTGEEVTF DELGGATTHT STSGVAHFAT DTEEQALDDI RHLLSYLPQN
260 270 280 290 300
NVEDPPRVEP WDDPERVADE LEEIVPDQPR KPYDIHDVLN GVLDEGSFFG
310 320 330 340 350
VQEDFAKNIV VGFGRLDGHS VGIVANQPRV NAGTLDIEAS EKGARFIRFC
360 370 380 390 400
DSFNIPILSF VDVPGFLPGT DQEHNGIIRH GAKLLYAYSE ATVPLMTVIT
410 420 430 440 450
RKAYGGAYDV MASKHLGADV NYAWPTAEIA VMGPQGAVNI LYRDELEAAD
460 470 480 490 500
DPDARRDELI EEYREEFANP YTAADRGFVD DVIEPGDTRN RLIADLRMLK
510
SKRKSQPDKK HGNIPL
Length:516
Mass (Da):56,903
Last modified:September 5, 2012 - v1
Checksum:iA875B0103A14FC39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001868 Genomic DNA. Translation: AFK20161.1.

Genome annotation databases

EnsemblBacteriaiAFK20161; AFK20161; HFX_2478.
KEGGihme:HFX_2478.

Similar proteinsi

Entry informationi

Entry nameiPCCB_HALMT
AccessioniPrimary (citable) accession number: I3R7F1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2017
Last sequence update: September 5, 2012
Last modified: August 30, 2017
This is version 32 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families