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I3R375

- I3R375_HALMT

UniProt

I3R375 - I3R375_HALMT

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 20 (01 Oct 2014)
      Sequence version 1 (05 Sep 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei165 – 1651Proton acceptorUniRule annotation
    Binding sitei167 – 1671SubstrateUniRule annotation
    Metal bindingi191 – 1911Magnesium; via carbamate groupUniRule annotation
    Metal bindingi193 – 1931MagnesiumUniRule annotation
    Metal bindingi194 – 1941MagnesiumUniRule annotation
    Active sitei283 – 2831Proton acceptorUniRule annotation
    Binding sitei284 – 2841SubstrateUniRule annotation
    Binding sitei316 – 3161SubstrateUniRule annotation
    Sitei324 – 3241Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: InterPro

    Keywords - Molecular functioni

    LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciHMED523841:GLDU-961-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotationImported
    Ordered Locus Names:HFX_0967Imported
    ORF Names:BM92_04375Imported, C439_05630Imported
    OrganismiHaloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei)Imported
    Taxonomic identifieri523841 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
    ProteomesiUP000006469: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei191 – 1911N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni352 – 3543Substrate bindingUniRule annotation
    Regioni374 – 3774Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family.UniRule annotation
    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    KOiK01601.
    OMAiVIVTFRV.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProiIPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    I3R375-1 [UniParc]FASTAAdd to Basket

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    MGITYEDFLD LEYEPTDEDL VCTFRIDPAT GMSMEAAASR VASESSNGTW    50
    AALQPGADFT DMGATTFSIA ERSSAGSQTG SDDIDGGNIQ VAYPAGLFEP 100
    GNMPQVLSCI AGNIMGMKAV DSIRLMDCEW PEPVVSSYPG PLYGSSVREE 150
    IFGVSDRPIT ATVPKPKVGL STAEHAQVGY DAWVGGVDLL KDDENLTDQD 200
    FNPFADRLTE SLSLRDDAED ETGEKKSYLI NVTADTQTML DRVDEVAAQG 250
    GEYVMVDIIT AGWASLQTVR ERTEKHGLAI HAHRAMHAAF DRMPTHGVSM 300
    RVLAQISRLC GVDQLHTGTA GLGKLANEDT VGINDWLKGD LYGRNDVLPV 350
    ASGGLHPGLL PDLLDATGTN TCIQLGGGIH GHPDGTRSGA IALRSAIDAY 400
    VEGKSITEAA EETPELAVAL DKWGTETPR 429
    Length:429
    Mass (Da):45,707
    Last modified:September 5, 2012 - v1
    Checksum:iC183A31F88DEC184
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001868 Genomic DNA. Translation: AFK18685.1.
    CP007551 Genomic DNA. Translation: AHZ21945.1.
    AOLO01000006 Genomic DNA. Translation: EMA03454.1.
    RefSeqiYP_006348672.1. NC_017941.1.

    Genome annotation databases

    EnsemblBacteriaiAFK18685; AFK18685; HFX_0967.
    EMA03454; EMA03454; C439_05630.
    GeneIDi13027133.
    KEGGihme:HFX_0967.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001868 Genomic DNA. Translation: AFK18685.1 .
    CP007551 Genomic DNA. Translation: AHZ21945.1 .
    AOLO01000006 Genomic DNA. Translation: EMA03454.1 .
    RefSeqi YP_006348672.1. NC_017941.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AFK18685 ; AFK18685 ; HFX_0967 .
    EMA03454 ; EMA03454 ; C439_05630 .
    GeneIDi 13027133.
    KEGGi hme:HFX_0967.

    Phylogenomic databases

    KOi K01601.
    OMAi VIVTFRV.

    Enzyme and pathway databases

    BioCyci HMED523841:GLDU-961-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProi IPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of the haloarchaeal phasin (PhaP) that functions in polyhydroxyalkanoate accumulation and granule formation in Haloferax mediterranei."
      Cai S., Cai L., Liu H., Liu X., Han J., Zhou J., Xiang H.
      Appl. Environ. Microbiol. 78:1946-1952(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: CGMCC 1.2087Imported.
    2. "Complete genome sequence of the metabolically versatile halophilic archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) producer."
      Han J., Zhang F., Hou J., Liu X., Li M., Liu H., Cai L., Zhang B., Chen Y., Zhou J., Hu S., Xiang H.
      J. Bacteriol. 194:4463-4464(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4Imported and CGMCC 1.2087Imported.
    3. Becker E.A., Seitzer P., Tritt A., Larsen D., Yao A., Wu D., Darling A., Eisen J.A., Facciotti M.T.
      Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ATCC 33500Imported.
    4. "Transcriptional profiles of Haloferax mediterranei on the basis of nitrogen availability."
      Bautista V.
      Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ATCC 33500Imported.
    5. Wang L., Yang H., Xiang H.
      Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: CGMCC 1.2087.

    Entry informationi

    Entry nameiI3R375_HALMT
    AccessioniPrimary (citable) accession number: I3R375
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 5, 2012
    Last sequence update: September 5, 2012
    Last modified: October 1, 2014
    This is version 20 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3