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I3LM39

- CGAS_PIG

UniProt

I3LM39 - CGAS_PIG

Protein

Cyclic GMP-AMP synthase

Gene

MB21D1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 14 (01 Oct 2014)
      Sequence version 1 (11 Jul 2012)
      Previous versions | rss
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    Functioni

    Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.1 Publication

    Catalytic activityi

    ATP + GTP = 2 diphosphate + cyclic G-P(2'-5')A-P(3'-5').1 Publication

    Cofactori

    Binds 1 Mg2+ per subunit.1 Publication

    Enzyme regulationi

    The enzyme activity is strongly increased by double-stranded DNA, but not by single-stranded DNA or RNA.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei186 – 1861GTP
    Binding sitei188 – 1881ATP
    Metal bindingi200 – 2001Magnesium; catalytic
    Metal bindingi202 – 2021Magnesium; catalytic
    Metal bindingi294 – 2941Magnesium; catalytic
    Binding sitei294 – 2941GTP
    Binding sitei358 – 3581ATP
    Metal bindingi365 – 3651Zinc
    Metal bindingi371 – 3711Zinc
    Metal bindingi372 – 3721Zinc
    Metal bindingi379 – 3791Zinc
    Binding sitei389 – 3891ATP

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi351 – 3588GTP
    Nucleotide bindingi410 – 4145ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclic-GMP-AMP synthase activity Source: Ensembl
    3. DNA binding Source: UniProtKB-KW
    4. GTP binding Source: UniProtKB-KW
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. activation of innate immune response Source: Ensembl
    2. cellular response to exogenous dsRNA Source: Ensembl
    3. cyclic nucleotide biosynthetic process Source: Ensembl
    4. defense response to virus Source: UniProtKB-KW
    5. innate immune response Source: UniProtKB-KW
    6. positive regulation of defense response to virus by host Source: Ensembl

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Antiviral defense, Immunity, Innate immunity

    Keywords - Ligandi

    ATP-binding, DNA-binding, GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_222145. STING mediated induction of host immune responses.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclic GMP-AMP synthase (EC:2.7.7.86)
    Short name:
    cGAMP synthase
    Short name:
    cGAS
    Alternative name(s):
    Mab-21 domain-containing protein 1
    Gene namesi
    Name:MB21D1
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Chromosome 1

    Subcellular locationi

    Cytoplasmcytosol By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi200 – 2001E → Q: Abolishes enzyme activity and stimulation of interferon production; when associated with N-202. 1 Publication
    Mutagenesisi202 – 2021D → N: Abolishes enzyme activity and stimulation of interferon production; when associated with Q-200. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 495495Cyclic GMP-AMP synthasePRO_0000423063Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei7 – 71N6-acetyllysineBy similarity
    Modified residuei389 – 3891N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Structurei

    Secondary structure

    1
    495
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi139 – 1479
    Helixi150 – 17324
    Turni177 – 1804
    Beta strandi182 – 1843
    Turni188 – 1925
    Beta strandi200 – 2089
    Beta strandi213 – 2175
    Beta strandi221 – 2277
    Helixi238 – 2403
    Helixi248 – 26215
    Beta strandi269 – 2713
    Beta strandi281 – 2855
    Beta strandi287 – 2893
    Beta strandi291 – 30111
    Helixi307 – 3093
    Turni316 – 3194
    Helixi321 – 3288
    Beta strandi332 – 3354
    Beta strandi339 – 3446
    Helixi346 – 3483
    Beta strandi350 – 3534
    Helixi355 – 3639
    Beta strandi366 – 3683
    Turni369 – 3724
    Helixi381 – 39818
    Turni399 – 4013
    Turni404 – 4074
    Helixi410 – 42314
    Helixi427 – 4293
    Helixi432 – 4343
    Helixi435 – 45218
    Turni468 – 4703
    Helixi473 – 48816
    Helixi492 – 4943

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4JLXX-ray2.00A135-495[»]
    4JLZX-ray2.27A/B135-495[»]
    4KB6X-ray3.08A135-495[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni147 – 19044DNA-bindingAdd
    BLAST
    Regioni359 – 38224DNA-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the mab-21 family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00710000106842.
    OMAiPQDSQWD.
    OrthoDBiEOG7S21ZG.
    TreeFamiTF331255.

    Family and domain databases

    InterProiIPR024810. Mab-21_dom.
    [Graphical view]
    PfamiPF03281. Mab-21. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    I3LM39-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAARRGKSTR TASEVGAAGP RASARSVNGA PTVPEAARPG ARRNGPSRAS    50
    GCRREKSGPD PREKPQVRTR TARAEDQAEG PSAPSERVEP PSAQGASLLR 100
    AGSCRAREAR SARELRPQAG ATELAAPARM EAPPGAWKLQ TVLEKVRLSR 150
    HEISEAAEVV NWVVEHLLRR LQGGESEFKG VALLRTGSYY ERVKISAPNE 200
    FDVMFKLEVP RIQLEEYCNS GAHYFVKFKR NPGGNPLEQF LEKEILSASK 250
    MLSKFRKIIK EEIKNIEGVT VERKRRGSPA VTLLISKPKE ISVDIILALE 300
    SKSSWPASTQ KGLPISQWLG AKVKNNLKRQ PFYLVPKHAK EGSGFQEETW 350
    RLSFSHIEKD ILKNHGQSKT CCEIDGVKCC RKECLKLMKY LLEQLKKKFG 400
    NRRELAKFCS YHVKTAFFHV CTQDPHDNQW HLKNLECCFD NCVAYFLQCL 450
    KTEQLANYFI PGVNLFSRDL IDKPSKEFLS KQIEYERNNG FPVFW 495
    Length:495
    Mass (Da):55,809
    Last modified:July 11, 2012 - v1
    Checksum:i13E76296D97E176B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FP102323 Genomic DNA. No translation available.

    Genome annotation databases

    EnsembliENSSSCT00000030573; ENSSSCP00000025159; ENSSSCG00000021383.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FP102323 Genomic DNA. No translation available.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4JLX X-ray 2.00 A 135-495 [» ]
    4JLZ X-ray 2.27 A/B 135-495 [» ]
    4KB6 X-ray 3.08 A 135-495 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSSSCT00000030573 ; ENSSSCP00000025159 ; ENSSSCG00000021383 .

    Phylogenomic databases

    GeneTreei ENSGT00710000106842.
    OMAi PQDSQWD.
    OrthoDBi EOG7S21ZG.
    TreeFami TF331255.

    Enzyme and pathway databases

    Reactomei REACT_222145. STING mediated induction of host immune responses.

    Family and domain databases

    InterProi IPR024810. Mab-21_dom.
    [Graphical view ]
    Pfami PF03281. Mab-21. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Porcine genome sequencing project
      Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 135-495 IN COMPLEXES WITH MAGNESIUM IONS, ZINC IONS; ATP; GTP; UTP AND DNA, COFACTOR, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, DNA-BINDING, MUTAGENESIS OF GLU-200 AND ASP-202.

    Entry informationi

    Entry nameiCGAS_PIG
    AccessioniPrimary (citable) accession number: I3LM39
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2013
    Last sequence update: July 11, 2012
    Last modified: October 1, 2014
    This is version 14 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3