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I3LM39

- CGAS_PIG

UniProt

I3LM39 - CGAS_PIG

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Protein

Cyclic GMP-AMP synthase

Gene

MB21D1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.1 Publication

Catalytic activityi

ATP + GTP = 2 diphosphate + cyclic G-P(2'-5')A-P(3'-5').1 Publication

Cofactori

Binds 1 Mg2+ per subunit.1 Publication

Enzyme regulationi

The enzyme activity is strongly increased by double-stranded DNA, but not by single-stranded DNA or RNA.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei186 – 1861GTP
Binding sitei188 – 1881ATP
Metal bindingi200 – 2001Magnesium; catalytic
Metal bindingi202 – 2021Magnesium; catalytic
Metal bindingi294 – 2941Magnesium; catalytic
Binding sitei294 – 2941GTP
Binding sitei358 – 3581ATP
Metal bindingi365 – 3651Zinc
Metal bindingi371 – 3711Zinc
Metal bindingi372 – 3721Zinc
Metal bindingi379 – 3791Zinc
Binding sitei389 – 3891ATP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi351 – 3588GTP
Nucleotide bindingi410 – 4145ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclic-GMP-AMP synthase activity Source: Ensembl
  3. DNA binding Source: UniProtKB-KW
  4. GTP binding Source: UniProtKB-KW
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. activation of innate immune response Source: Ensembl
  2. cellular response to exogenous dsRNA Source: Ensembl
  3. cyclic nucleotide biosynthetic process Source: Ensembl
  4. defense response to virus Source: UniProtKB-KW
  5. innate immune response Source: UniProtKB-KW
  6. positive regulation of defense response to virus by host Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

ATP-binding, DNA-binding, GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_222145. STING mediated induction of host immune responses.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic GMP-AMP synthase (EC:2.7.7.86)
Short name:
cGAMP synthase
Short name:
cGAS
Alternative name(s):
Mab-21 domain-containing protein 1
Gene namesi
Name:MB21D1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 1

Subcellular locationi

Cytoplasmcytosol By similarity

GO - Cellular componenti

  1. cytosol Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi200 – 2001E → Q: Abolishes enzyme activity and stimulation of interferon production; when associated with N-202. 1 Publication
Mutagenesisi202 – 2021D → N: Abolishes enzyme activity and stimulation of interferon production; when associated with Q-200. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495Cyclic GMP-AMP synthasePRO_0000423063Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei7 – 71N6-acetyllysineBy similarity
Modified residuei389 – 3891N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Structurei

Secondary structure

1
495
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi139 – 1479Combined sources
Helixi150 – 17324Combined sources
Turni177 – 1804Combined sources
Beta strandi182 – 1843Combined sources
Turni188 – 1925Combined sources
Beta strandi200 – 2089Combined sources
Beta strandi213 – 2175Combined sources
Beta strandi221 – 2277Combined sources
Helixi238 – 2403Combined sources
Helixi248 – 26215Combined sources
Beta strandi269 – 2713Combined sources
Beta strandi281 – 2855Combined sources
Beta strandi287 – 2893Combined sources
Beta strandi291 – 30111Combined sources
Helixi307 – 3093Combined sources
Turni316 – 3194Combined sources
Helixi321 – 3288Combined sources
Beta strandi332 – 3354Combined sources
Beta strandi339 – 3446Combined sources
Helixi346 – 3483Combined sources
Beta strandi350 – 3534Combined sources
Helixi355 – 3639Combined sources
Beta strandi366 – 3683Combined sources
Turni369 – 3724Combined sources
Helixi381 – 39818Combined sources
Turni399 – 4013Combined sources
Turni404 – 4074Combined sources
Helixi410 – 42314Combined sources
Helixi427 – 4293Combined sources
Helixi432 – 4343Combined sources
Helixi435 – 45218Combined sources
Turni468 – 4703Combined sources
Helixi473 – 48816Combined sources
Helixi492 – 4943Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4JLXX-ray2.00A135-495[»]
4JLZX-ray2.27A/B135-495[»]
4KB6X-ray3.08A135-495[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni147 – 19044DNA-bindingAdd
BLAST
Regioni359 – 38224DNA-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the mab-21 family.Curated

Phylogenomic databases

GeneTreeiENSGT00710000106842.
InParanoidiI3LM39.
OMAiPQDSQWD.
OrthoDBiEOG7S21ZG.
TreeFamiTF331255.

Family and domain databases

InterProiIPR024810. Mab-21_dom.
[Graphical view]
PfamiPF03281. Mab-21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

I3LM39-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAARRGKSTR TASEVGAAGP RASARSVNGA PTVPEAARPG ARRNGPSRAS
60 70 80 90 100
GCRREKSGPD PREKPQVRTR TARAEDQAEG PSAPSERVEP PSAQGASLLR
110 120 130 140 150
AGSCRAREAR SARELRPQAG ATELAAPARM EAPPGAWKLQ TVLEKVRLSR
160 170 180 190 200
HEISEAAEVV NWVVEHLLRR LQGGESEFKG VALLRTGSYY ERVKISAPNE
210 220 230 240 250
FDVMFKLEVP RIQLEEYCNS GAHYFVKFKR NPGGNPLEQF LEKEILSASK
260 270 280 290 300
MLSKFRKIIK EEIKNIEGVT VERKRRGSPA VTLLISKPKE ISVDIILALE
310 320 330 340 350
SKSSWPASTQ KGLPISQWLG AKVKNNLKRQ PFYLVPKHAK EGSGFQEETW
360 370 380 390 400
RLSFSHIEKD ILKNHGQSKT CCEIDGVKCC RKECLKLMKY LLEQLKKKFG
410 420 430 440 450
NRRELAKFCS YHVKTAFFHV CTQDPHDNQW HLKNLECCFD NCVAYFLQCL
460 470 480 490
KTEQLANYFI PGVNLFSRDL IDKPSKEFLS KQIEYERNNG FPVFW
Length:495
Mass (Da):55,809
Last modified:July 11, 2012 - v1
Checksum:i13E76296D97E176B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FP102323 Genomic DNA. No translation available.

Genome annotation databases

EnsembliENSSSCT00000030573; ENSSSCP00000025159; ENSSSCG00000021383.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FP102323 Genomic DNA. No translation available.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4JLX X-ray 2.00 A 135-495 [» ]
4JLZ X-ray 2.27 A/B 135-495 [» ]
4KB6 X-ray 3.08 A 135-495 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000030573 ; ENSSSCP00000025159 ; ENSSSCG00000021383 .

Phylogenomic databases

GeneTreei ENSGT00710000106842.
InParanoidi I3LM39.
OMAi PQDSQWD.
OrthoDBi EOG7S21ZG.
TreeFami TF331255.

Enzyme and pathway databases

Reactomei REACT_222145. STING mediated induction of host immune responses.

Family and domain databases

InterProi IPR024810. Mab-21_dom.
[Graphical view ]
Pfami PF03281. Mab-21. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Porcine genome sequencing project
    Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 135-495 IN COMPLEXES WITH MAGNESIUM IONS, ZINC IONS; ATP; GTP; UTP AND DNA, COFACTOR, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, DNA-BINDING, MUTAGENESIS OF GLU-200 AND ASP-202.

Entry informationi

Entry nameiCGAS_PIG
AccessioniPrimary (citable) accession number: I3LM39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: July 11, 2012
Last modified: October 29, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3