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I2EMA1 (I2EMA1_CROSK) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
ORF Names:ES15_3230 EMBL AFK00804.1
OrganismCronobacter sakazakii ES15 [Complete proteome] EMBL AFK00804.1
Taxonomic identifier1138308 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain101 – 303203ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding134 – 18956ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2571Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2701Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2701Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2721Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I2EMA1 [UniParc].

Last modified July 11, 2012. Version 1.
Checksum: 64AF4107427F15AD

FASTA30632,805
        10         20         30         40         50         60 
MAEKIAVLLG GHSAERDVSL QSGSAVLAGL KEAGVDAYAV DPRDVCVTTL KEEGFDKVFI 

        70         80         90        100        110        120 
ALHGRGGEDG TLQGMLEYLG LPYTGSGVMA SAITMDKLRT KLLWQGAGLP VAQWVALERQ 

       130        140        150        160        170        180 
DITGGLSEAL SARIAALGLP VIVKPSREGS SVGMSKVDSA EALLPALELA FEHDSEVLVE 

       190        200        210        220        230        240 
KWLSGPEFTV AILGDEILPS IRIQPAGVFY DYEAKYLSDE TQYFCPAGID AEREQALQAL 

       250        260        270        280        290        300 
VMDAWRVLGC RGWGRVDVMQ DSDGEFYLLE VNTAPGMTSH SLVPMAARQA GMTFSQLVVR 


ILELAN 

« Hide

References

[1]"Complete Genome Sequence of the Opportunistic Food-Borne Pathogen Cronobacter sakazakii ES15."
Shin H., Lee J.H., Choi Y., Ryu S.
J. Bacteriol. 194:4438-4439(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ES15 EMBL AFK00804.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003312 Genomic DNA. Translation: AFK00804.1.
RefSeqYP_006344314.1. NC_017933.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFK00804; AFK00804; ES15_3230.
GeneID13034412.
KEGGcsk:ES15_3230.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycCSAK1138308:GLC5-3303-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI2EMA1_CROSK
AccessionPrimary (citable) accession number: I2EMA1
Entry history
Integrated into UniProtKB/TrEMBL: July 11, 2012
Last sequence update: July 11, 2012
Last modified: June 11, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)