ID   I2DT64_9BURK            Unreviewed;       659 AA.
AC   I2DT64;
DT   11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT   11-JUL-2012, sequence version 1.
DT   24-JAN-2024, entry version 39.
DE   RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE            Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE            EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN   ORFNames=MYA_3481 {ECO:0000313|EMBL:AFJ87840.1};
OS   Burkholderia sp. KJ006.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia.
OX   NCBI_TaxID=416344 {ECO:0000313|EMBL:AFJ87840.1, ECO:0000313|Proteomes:UP000010108};
RN   [1] {ECO:0000313|EMBL:AFJ87840.1, ECO:0000313|Proteomes:UP000010108}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KJ006 {ECO:0000313|EMBL:AFJ87840.1,
RC   ECO:0000313|Proteomes:UP000010108};
RX   PubMed=22843575; DOI=10.1128/JB.00821-12;
RA   Kwak M.J., Song J.Y., Kim S.Y., Jeong H., Kang S.G., Kim B.K., Kwon S.K.,
RA   Lee C.H., Yu D.S., Park S.H., Kim J.F.;
RT   "Complete Genome Sequence of the Endophytic Bacterium Burkholderia sp.
RT   Strain KJ006.";
RL   J. Bacteriol. 194:4432-4433(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC         Evidence={ECO:0000256|ARBA:ARBA00001412,
CC         ECO:0000256|PIRNR:PIRNR001084};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC       {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
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DR   EMBL; CP003515; AFJ87840.1; -; Genomic_DNA.
DR   RefSeq; WP_014724277.1; NC_017921.1.
DR   AlphaFoldDB; I2DT64; -.
DR   KEGG; buk:MYA_3481; -.
DR   PATRIC; fig|416344.3.peg.3538; -.
DR   HOGENOM; CLU_012430_1_0_4; -.
DR   Proteomes; UP000010108; Chromosome 2.
DR   GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR013738; Beta_galactosidase_Trimer.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR003476; Glyco_hydro_42.
DR   InterPro; IPR013529; Glyco_hydro_42_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR   PANTHER; PTHR36447:SF2; BETA-GALACTOSIDASE YESZ; 1.
DR   Pfam; PF02449; Glyco_hydro_42; 1.
DR   Pfam; PF08532; Glyco_hydro_42M; 1.
DR   PIRSF; PIRSF001084; B-galactosidase; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001084}.
FT   DOMAIN          6..389
FT                   /note="Glycoside hydrolase family 42 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF02449"
FT   DOMAIN          402..601
FT                   /note="Beta-galactosidase trimerisation"
FT                   /evidence="ECO:0000259|Pfam:PF08532"
FT   ACT_SITE        142
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT   ACT_SITE        313
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT   BINDING         103
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT   BINDING         141
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT   BINDING         321
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ   SEQUENCE   659 AA;  73771 MW;  DC105922D156155C CRC64;
     MRLGVCYYPE HWPESMWADD ARRMKALGIV QVRIGEFAWS RIEPSPGEYA WGWLDRAIDA
     LGAAGLEIVM CTPTATPPKW LVDRHPDILA IGADGLPRKF GSRRHYDFSS PTYLEASRRI
     CTAVAERYGR HPAVRYWQTD NELGCHQTVV SYSPAALLRF RAWLKARYGT IDALNRAWGT
     VFWSMEYRHF DEVDAPVGTV TEAHPSHRLD YRRFASDELA RYHRMQVDVI RAHSPGRPVA
     HNFMQLFTEF DHYAVARDLD VATWDSYPLG ALEELWFAPD VKARWLRTGH PDFASFNHDL
     YRGMSKQPFW VMEQQPGPVN WAHWNPAPLP GMVRLWSWEA FAHGAGCVSY FRWRQAPFAQ
     EQMHAGLHTP DDRLDEGGRE AQRVAGEIAA VLDADADGAV RAPVALVFDY EAKWLLEVQP
     QGADFHYPRF AFEYYSALRA LGLDVDIVSA HAPLDGYRLV VVPPLPVVPD DFAARLAASG
     AHAVFGPRTG SKTVDLQIPP ALPPGPLASL LPLRVWRVES LRPNVTERID GALRDGSPLS
     GDARHWRDLV ELHDDARSVV HARFADGHPA WVSHGAFHYW AALFDDATTL RGFADVAAEA
     GLTPTPLGDD VRVSRRGGLT YVFNYGSTPH TIDAVAPSAF VVGAAQVEPQ GVAVYRSRP
//