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I2C1K9 (I2C1K9_BACAM) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AFJ60533.1
ORF Names:MUS_0459 EMBL AFJ60533.1
OrganismBacillus amyloliquefaciens Y2 [Complete proteome] EMBL AFJ60533.1
Taxonomic identifier1126211 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain133 – 338206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding166 – 22156ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2921Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3051Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3051Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3071Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
I2C1K9 [UniParc].

Last modified July 11, 2012. Version 1.
Checksum: DB7EA78B5E44C06A

FASTA35439,217
        10         20         30         40         50         60 
MYGGKSAEHN VSLQTALAVT KALNTEKFDI HPIYITEKGE WQRGPLLTEP VSNVKMLQLE 

        70         80         90        100        110        120 
QNGESFALSA LNREMFPEAS PDEKAPIDVV FPLLHGPNGE DGTIQGLLEL LNVPYVGNGV 

       130        140        150        160        170        180 
LASSAGMDKV IMKHLFAQAG LPQAKYAAFL KKDWKQTPDS CLQQVEEELG YPCFVKPANL 

       190        200        210        220        230        240 
GSSVGISKCR NREELEKAFE LAFEYDRKIV VEEGIAGREI EIGVLGNDEP KCSAVGEIAP 

       250        260        270        280        290        300 
KTDFYDYKAK YEDGDTDLMI PAQVTDEQYA EISEMAIKAF KAIDGSGLVR ADFFLTNDGQ 

       310        320        330        340        350 
VLINEVNTMP GFTPFSMFPL LWKEAGVDYS DLIEQLVELA KERHAEKQLI KHTF 

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References

[1]"Genome sequence of the plant growth promoting strain Bacillus amyloliquefaciens subsp. plantarum B9601-Y2 and expression of mersacidin and other secondary metabolites."
He P., Hao K., Blom J., Ruckert C., Vater J., Mao Z., Wu Y., Hou M., He P., He Y., Borriss R.
J. Biotechnol. 164:281-291(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Y2 EMBL AFJ60533.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003332 Genomic DNA. Translation: AFJ60533.1.
RefSeqYP_006327258.1. NC_017912.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFJ60533; AFJ60533; MUS_0459.
GeneID12963592.
KEGGbqy:MUS_0459.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycBAMY1126211:GL8D-485-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameI2C1K9_BACAM
AccessionPrimary (citable) accession number: I2C1K9
Entry history
Integrated into UniProtKB/TrEMBL: July 11, 2012
Last sequence update: July 11, 2012
Last modified: February 19, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)